10x ELISA Wash Buffer

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10x ELISA Wash Buffer

Product Type
Accessory Reagent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
BUF031A E 100 ml
BUF031B E 500 ml
BUF031C E 1000 ml

Product Details

Intended Use
ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

BUF031A is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
Product Form
Pack Size: 100 ml, 1000 ml
10 x concentration - liquid
Pack Size: 500 ml
10x concentrate - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1.0% 2-chloroacetamide

Storage Information

Storage
Pack Size: 100 ml, 500 ml
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 1000 ml
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Shelf Life
Please see label for expiry date.

More Information

Regulatory
For research purposes only

Applications of 10x ELISA Wash Buffer

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Instructions For Use
1. Dilute the required volume of BUF031A 1:10 with deionised water (1 part BUF031A to 9 parts water) and mix.

2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
5x ELISA Coating Buffer BUF030A 100 ml E
ELISA BSA Block BUF032A 100 ml E