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10x ELISA Wash Buffer

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A microtiter plate was coated over night with MCA2112 at concentration of 5μg/ml in BUF030A. After washing with BUF031A and blocking with BUF032A samples were detected using MCA1783B antibody at 5μg/ml followed by incubation with streptavidin:HRP conjugate STAR5B at 1/1000. Finally, BUF056A was used as substrate. Data is presented as the mean of three measurements.

10x ELISA Wash Buffer

Product Type
Accessory Reagent
Specificity
10x ELISA Wash Buffer

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Product Code Applications Pack Size List Price Your Price Qty
BUF031C
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1000 ml loader
List Price Your Price
loader
BUF031B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 500 ml loader
List Price Your Price
loader
BUF031A
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 100 ml loader
List Price Your Price
loader

Intended Use
BUF031C
ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

BUF031C is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
BUF031B
ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

BUF031B is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
BUF031A
ELISA wash buffer was developed as a high performance washing solution for use in all ELISA formats. The optimised formulation of pH stabilizers, salts and detergents increases the efficiency of washing stages during ELISA, leading to a reduction in background noise and an increased specific signal.

BUF031A is compatible with all routinely used conjugate components such as HRP, Avidin and Alkaline Phosphatase.
Product Form
BUF031C, BUF031A: 10 x concentration - liquid
BUF031B: 10x concentrate - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1.0% 2-chloroacetamide
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Pack Size: 1000 ml
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.
Pack Size: 500 ml, 100 ml
Store at +4oC.

DO NOT FREEZE

This product should be stored undiluted. Should this product contain a precipitate, heat gently until dissolved. Do not boil.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Instructions For Use
BUF031C
1. Dilute the required volume of BUF031C 1:10 with deionised water (1 part BUF031C to 9 parts water) and mix.

2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
BUF031B
1. Dilute the required volume of BUF031B 1:10 with deionised water (1 part BUF031B to 9 parts water) and mix.

2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.
BUF031A
1. Dilute the required volume of BUF031A 1:10 with deionised water (1 part BUF031A to 9 parts water) and mix.

2. Use at each ELISA wash step for a total of 2-4 washes. Fill each well with the diluted wash buffer (approximately 400ul), taking care not to submerge the plate as this can cross-contaminate the wells. The buffer may be dispensed manually or by an automated system.

3. Between washes, aspirate or dump the buffer. After the final wash blot the plate on paper towels to remove any excess liquid.

Description Product Code Applications Pack Size List Price Your Price Quantity
5x ELISA Coating Buffer BUF030A E 100 ml
List Price Your Price
Description 5x ELISA Coating Buffer
ELISA BSA Block BUF032A E 100 ml
List Price Your Price
Description ELISA BSA Block

References for 10x ELISA Wash Buffer

  1. Vanpouille, C. et al. (2024) HIV-1 Nef is carried on the surface of extracellular vesicles.
    J Extracell Vesicles. 13 (7): e12478.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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