CD36 antibody | SMØ
Mouse anti Human CD36
- Product Type
- Monoclonal Antibody
- Clone
- SMØ
- Isotype
- IgM
- Specificity
- CD36
Antibody Quality and Performance Guarantee
| Mouse anti Human CD36 antibody, clone SMØ recognizes the CD36 antigen, an ~88 kDa cell surface glycoprotein expressed by platelets, monocytes/macrophages and also by some microvascular endothelium and erythroid precursors. CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages, CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. Mouse anti Human CD36 antibody, clone SMØ partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding, P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team. Mouse anti Human CD36 antibody, clone SMØ is also reported to block interaction of oxidized low-density lipoprotein (OXLDL) with CD36 (Sugihara et al.1992). |
- Target Species
- Human
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human tonsil cells and peripheral blood mononuclear cells.
- Approx. Protein Concentrations
- IgM concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the NSI mouse myeloma line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/20 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | ||||||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgM Negative Control | MCA692 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgM Negative Control | ||||||
References for CD36 antibody
-
Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
Immunology. 53 (4): 753-67. -
Pain, A. et al. (2001) Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria.
Proc Natl Acad Sci U S A. 98: 1805-10. -
Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.
J Cell Physiol. 202: 731-42. -
Biswas, A.K. et al. (2007) Plasmodium falciparum uses gC1qR/HABP1/p32 as a receptor to bind to vascular endothelium and for platelet-mediated clumping.
PLoS Pathog. 3: 1271-80. -
Sarigianni, M. et al. (2010) Effect of leptin and insulin resistance on properties of human monocytes in lean and obese healthy participants.
Angiology. 61: 768-74.
Further Reading
-
Greenwalt, D.E. et al. (1992) Membrane glycoprotein CD36: a review of its roles in adherence, signal transduction, and transfusion medicine.
Blood. 80 (5): 1105-15. -
Endemann, G. et al. (1993) CD36 is a receptor for oxidized low density lipoprotein.
J Biol Chem. 268 (16): 11811-6.
MCA722GA
1805
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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