CD36 antibody | SMØ

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Mouse anti Human CD36:Alexa Fluor® 647

Mouse anti Human CD36:Preservative Free

Mouse anti Human CD36:FITC

Mouse anti Human CD36

Product Type
Monoclonal Antibody
Clone
SMØ
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA722A647 F 100 Tests/1ml
MCA722XZ C F FN 1 mg
MCA722F F 0.1 mg
MCA722T C F 25 µg
MCA722GA C F 0.1 mg
MCA722 C F 0.2 mg
Mouse anti Human CD36 antibody, clone SMØ recognizes the CD36 antigen, an ~88 kDa cell surface glycoprotein expressed by platelets, monocytes/macrophages and also by some microvascular endothelium and erythroid precursors.

CD36 is the receptor for thrombospondin. When expressed by monocytes and macrophages, CD36 acts as a receptor for oxidized LDL and for apoptotic polymorphonuclear cells. Mouse anti Human CD36 antibody, clone SMØ partially inhibits collagen induced platelet aggregation and macrophage ingestion of apoptotic human neutrophils. It has been reported that this antibody may activate platelets as measured by fibrinogen binding, P-Selectin expression and Annexin V binding - for further details please contact our Technical Services team.

Mouse anti Human CD36 antibody, clone SMØ is also reported to block interaction of oxidised low-density lipoprotein (OXLDL) with CD36 (Sugihara et al.1992).

Product Details

Target Species
Human
Product Form
Purified IgM conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgM - liquid
Product Form
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Preparation
Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human tonsil cells and peripheral blood mononuclear cells.
Approx. Protein Concentrations
IgM concentration 0.05 mg/ml
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Approx. Protein Concentrations
IgM concentration 0.1 mg/ml
Approx. Protein Concentrations
IgM concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the NSI mouse myeloma line.

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20o only.

This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the protein.

Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P16671 Related reagents
Entrez Gene
CD36 Related reagents
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD36 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Flow Cytometry 1/25 1/50
Functional Assays
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Flow Cytometry Neat 1/10
Flow Cytometry 1/50 Pack Size: 25 µg, 0.2 mg
1/10 Pack Size: 0.1 mg
1/100 Pack Size: 25 µg, 0.2 mg
1/20 Pack Size: 0.1 mg
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Human anti Mouse IgM:FITC HCA040F 0.1 mg F
Human anti Mouse IgM:HRP HCA040P 0.1 mg E
Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) STAR138A 1 ml C E P WB
Goat anti Mouse IgM:RPE STAR86PE 0.5 ml F
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgM Negative Control MCA692 100 Tests F
Mouse IgM Negative Control:FITC MCA692F 0.1 mg F
Mouse IgM Negative Control MCA692 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

Functional Assays

Product Specific References

References for CD36 antibody

  1. Hogg, N. et al. (1984) Monoclonal antibodies specific for human monocytes, granulocytes and endothelium.
    Immunology. 53 (4): 753-67.
  2. Sarigianni, M. et al. (2010) Effect of leptin and insulin resistance on properties of human monocytes in lean and obese healthy participants.
    Angiology. 61: 768-74.
  3. Hogg, N. et al. (1985) Mononuclear phagocytes of normal and rheumatoid synovial membrane identified by monoclonal antibodies.
    Immunology. 56 (4): 673-81.
  4. Hooper, C. et al. (2009) Scavenger receptor control of chromogranin A-induced microglial stress and neurotoxic cascades.
    FEBS Lett. 583: 3461-6.
  5. Endemann, G. et al. (1993) CD36 is a receptor for oxidized low density lipoprotein.
    J Biol Chem. 268 (16): 11811-6.
  6. Sugihara, K. et al. (1992) Thrombospondin mediates adherence of CD36+ sickle reticulocytes to endothelial cells.
    Blood. 80 (10): 2634-42.
  7. Biswas, A.K. et al. (2007) Plasmodium falciparum uses gC1qR/HABP1/p32 as a receptor to bind to vascular endothelium and for platelet-mediated clumping.
    PLoS Pathog. 3: 1271-80.
  8. Pain, A. et al. (2001) Platelet-mediated clumping of Plasmodium falciparum-infected erythrocytes is a common adhesive phenotype and is associated with severe malaria.
    Proc Natl Acad Sci U S A. 98: 1805-10.
  9. Urban, B.C. et al. (2001) A role for CD36 in the regulation of dendritic cell function.
    Proc Natl Acad Sci U S A. 98: 8750-5.
  10. Diaz-Romero, J. et al. (2005) Immunophenotypic analysis of human articular chondrocytes: changes in surface markers associated with cell expansion in monolayer culture.
    J Cell Physiol. 202: 731-42.

Further Reading

  1. Greenwalt, D.E. et al. (1992) Membrane glycoprotein CD36: a review of its roles in adherence, signal transduction, and transfusion medicine.
    Blood. 80 (5): 1105-15.