CD36 antibody | MF3
Rat anti Mouse CD36 antibody, clone MF3 (MCA2748) used as a blocking antibody in α/β interferon induction experiments.
Image caption:
Scavenger receptor ligands do not inhibit IFNα/β production by influenza virus stimulated pDC. (A) Spleen cells were pre-incubated in media alone (no Ab), rat IgG2a or MF3 (anti-CD36 mAb) (antibodies at 100 μg/ml) and then stimulated with 530 HAU/ml inactivated influenza virus or media alone for 6 h. IFNα/β production by pDC was measured by intracellular staining (n = 3). (B) 1 × 107 spleen cells/ml were pre-incubated with media alone (none), maleylated-BSA, BSA (control for maleylated-BSA) or fucoidan (all at 100 μg/ml) and then stimulated for 6 h with 200 HAU/ml inactivated influenza virus (gray bars) or media alone (white bars) (n = 2). IFNα and IFNβ production by pDC was measured by intracellular staining.
From: Seeds RE, Mukhopadhyay S, Jones IM, Gordon S, Miller JL.
The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
Int Immunopharmacol. 2011 Jul;11(7):794-801.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD36 antibody, clone MF3 (MCA2748) used for detection of CD36 expressing cells in murine aortic tissue by immunohistochemistry on formalin fixed, paraffin embedded tissue sections. Sections are also stained for macrophages using Rat anti Mouse CD68 antibody, clone FA-11 (MCA1957).
Image caption:
Plaque lesions express macrophages and CD36 receptors. Aortas were stained with H&E (left), rat anti-mouse CD36 (middle), rat anti-mouse CD68 (bottom) or non-specific rat IgG (nsIgG). Peroxidase-conjugated anti-rat Abs were added and developed using AEC. The brown staining represents CD36- or CD68-positive cells located in the plaque lesions. Results are representative of at least three separate aortas.
From: Dellinger A, Olson J, Link K, Vance S, Sandros MG, Yang J, Zhou Z, Kepley CL.
Functionalization of gadolinium metallofullerenes for detecting atherosclerotic plaque lesions by cardiovascular magnetic resonance.
J Cardiovasc Magn Reson. 2013 Jan 16;15(1):7.
doi: 10.1186/1532-429X-15-7.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD36
- Product Type
- Monoclonal Antibody
- Clone
- MF3
- Isotype
- IgG2a
- Specificity
- CD36
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD36 antibody, clone MF3 recognizes mouse CD36, also known as platelet glycoprotein 4, glycoprotein IIIb or PAS IV. CD36 is an ~85 kDa multipass transmembrane glycoprotein primarily expressed on platelets, monocytes/macrophages, smooth muscle and endothelial cells. The CD36 molecule is type B scavenger receptor, which binds to multiple ligands including thrombospondin, anionic phospholipids, oxidized low density lipoproteins and long chain fatty acids. CD36 has diverse functions and is reported to play a role in innate immunity, platelet adhesion/aggregation and long chain fatty acid transport. The CD36 molecule also directly mediates cytoadhesion of erythrocytes infected with Plasmodium falciparum, and may be involved in the development of atherosclerotic lesions and the formation of foam cells. Rat anti Mouse CD36 antibody, clone MF3 has been shown to inhibit IL-4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL-4/GM-CSF-induced bone-marrorw derived macrophage fusion. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- IL-4 treated murine thioglycollate-elicited peritoneal macrophages
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the Y3Ag 1.2.3 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/25 |
| Immunofluorescence | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Helming, L. et al. (2009) The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion.
J Cell Sci. 122 (Pt 4): 453-9.
References for CD36 antibody
-
Mwaikambo, B.R. et al. (2009) Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms.
J Biol Chem. 284: 26695-707. -
Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
Int Immunopharmacol. 11: 794-801. -
Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
Neurobiol Dis. 42 (3): 221-30. -
Zhou, D. et al. (2012) CD36 level and trafficking are determinants of lipolysis in adipocytes.
FASEB J. 26 (11): 4733-42. -
Samovski, D. et al. (2012) Insulin and AMPK regulate FA translocase/CD36 plasma membrane recruitment in cardiomyocytes via Rab GAP AS160 and Rab8a Rab GTPase.
J Lipid Res. 53 (4): 709-17. -
Dellinger, A. et al. (2013) Functionalization of gadolinium metallofullerenes for detecting atherosclerotic plaque lesions by cardiovascular magnetic resonance.
J Cardiovasc Magn Reson. 15: 7. -
Miyazaki H et al. (2014) Fatty acid binding protein 7 regulates phagocytosis and cytokine production in Kupffer cells during liver injury.
Am J Pathol. 184 (9): 2505-15. -
Pugholm, L.H. et al. (2015) Enhanced Humoral Responses Induced by Targeting of Antigen to Murine Dendritic Cells.
Scand J Immunol. 82 (6): 515-22.
View The Latest Product References
-
Samovski, D. et al. (2015) Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation.
Diabetes. 64 (2): 353-9. -
Nie, S. et al. (2015) Detection of atherosclerotic lesions and intimal macrophages using CD36-targeted nanovesicles.
J Control Release. pii: S0168-3659(15)30173-5. -
Lund, H. et al. (2018) Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells.
Nat Commun. 9 (1): 4845. -
Sui, Y. et al. (2018) Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice.
Endocrinology. 159 (4): 1595-608. -
Zhang, J. et al. (2019) Anti-atherogenic effects of CD36-targeted epigallocatechin gallate-loaded nanoparticles.
J Control Release. 303: 263-73. -
Dhanasekara, C.S. et al. (2021) Nanoparticles target intimal macrophages in atherosclerotic lesions.
Nanomedicine. 32: 102346. -
Liu, J. et al. (2022) Pregnane X Receptor Mediates Atherosclerosis Induced by Dicyclohexyl Phthalate in LDL Receptor-Deficient Mice
Cells. 11 (7): 1125
- Synonyms
- GPIV (IIIb)
- RRID
- AB_2072640
- UniProt
- Q08857
- Entrez Gene
- Cd36
- GO Terms
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005041 low-density lipoprotein receptor activity
- GO:0005794 Golgi apparatus
- GO:0008289 lipid binding
- GO:0006910 phagocytosis, recognition
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0008035 high-density lipoprotein particle binding
- GO:0050830 defense response to Gram-positive bacterium
- View More GO Terms
- GO:0009897 external side of plasma membrane
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
- GO:0010671 negative regulation of oxygen and reactive oxygen species metabolic process
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010886 positive regulation of cholesterol storage
- GO:0019915 lipid storage
- GO:0023048 signal initiation by lipid mediator
- GO:0030194 positive regulation of blood coagulation
- GO:0030301 cholesterol transport
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0032760 positive regulation of tumor necrosis factor production
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0034383 low-density lipoprotein particle clearance
- GO:0035634 response to stilbenoid
- GO:0042953 lipoprotein transport
- GO:0042992 negative regulation of transcription factor import into nucleus
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0043277 apoptotic cell clearance
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0051636 Gram-negative bacterial cell surface binding
- GO:0051637 Gram-positive bacterial cell surface binding
- GO:0060100 positive regulation of phagocytosis, engulfment
- GO:0060907 positive regulation of macrophage cytokine production
- GO:0070892 lipoteichoic acid receptor activity
- GO:0071221 cellular response to bacterial lipopeptide
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071223 cellular response to lipoteichoic acid
- GO:0071447 cellular response to hydroperoxide
- GO:2000121 regulation of removal of superoxide radicals
MCA2748GA
1708
MCA2748
149362
160795
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