CD36 antibody | MF3

Product Code Applications Pack Size List Price Your Price Qty
MCA2748GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF WB 0.1 mg loader
List Price Your Price
loader
MCA2748
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF WB 0.25 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD36 antibody, clone MF3 recognizes mouse CD36, also known as platelet glycoprotein 4, glycoprotein IIIb or PAS IV. CD36 is an ~85 kDa multipass transmembrane glycoprotein primarily expressed on platelets, monocytes/macrophages, smooth muscle and endothelial cells. The CD36 molecule is type B scavenger receptor, which binds to multiple ligands including thrombospondin, anionic phospholipids, oxidized low density lipoproteins and long chain fatty acids.

CD36 has diverse functions and is reported to play a role in innate immunity, platelet adhesion/aggregation and long chain fatty acid transport. The CD36 molecule also directly mediates cytoadhesion of erythrocytes infected with Plasmodium falciparum, and may be involved in the development of atherosclerotic lesions and the formation of foam cells.

Rat anti Mouse CD36 antibody, clone MF3 has been shown to inhibit IL-4 induced thioglycollate-elicited peritoneal macrophage fusion and significantly block IL-4/GM-CSF-induced bone-marrorw derived macrophage fusion.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
IL-4 treated murine thioglycollate-elicited peritoneal macrophages
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the Y3Ag 1.2.3 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/25
Immunofluorescence
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
List Price Your Price
loader
Description Rat IgG2a Negative Control

References for CD36 antibody

  1. Helming, L. et al. (2009) The scavenger receptor CD36 plays a role in cytokine-induced macrophage fusion.
    J Cell Sci. 122 (Pt 4): 453-9.
  2. Mwaikambo, B.R. et al. (2009) Hypoxia up-regulates CD36 expression and function via hypoxia-inducible factor-1- and phosphatidylinositol 3-kinase-dependent mechanisms.
    J Biol Chem. 284: 26695-707.
  3. Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
    Neurobiol Dis. 42 (3): 221-30.
  4. Seeds, R.E. et al. (2011) The role of myeloid receptors on murine plasmacytoid dendritic cells in induction of type I interferon.
    Int Immunopharmacol. 11: 794-801.
  5. Samovski, D. et al. (2012) Insulin and AMPK regulate FA translocase/CD36 plasma membrane recruitment in cardiomyocytes via Rab GAP AS160 and Rab8a Rab GTPase.
    J Lipid Res. 53 (4): 709-17.
  6. Zhou, D. et al. (2012) CD36 level and trafficking are determinants of lipolysis in adipocytes.
    FASEB J. 26 (11): 4733-42.
  7. Nie, S. et al. (2015) Detection of atherosclerotic lesions and intimal macrophages using CD36-targeted nanovesicles.
    J Control Release. pii: S0168-3659(15)30173-5.
  8. Samovski, D. et al. (2015) Regulation of AMPK activation by CD36 links fatty acid uptake to β-oxidation.
    Diabetes. 64 (2): 353-9.
  9. View The Latest Product References
  10. Pugholm, L.H. et al. (2015) Enhanced Humoral Responses Induced by Targeting of Antigen to Murine Dendritic Cells.
    Scand J Immunol. 82 (6): 515-22.
  11. Miyazaki H et al. (2014) Fatty acid binding protein 7 regulates phagocytosis and cytokine production in Kupffer cells during liver injury.
    Am J Pathol. 184 (9): 2505-15.
  12. Sui, Y. et al. (2018) Perinatal Bisphenol A Exposure Increases Atherosclerosis in Adult Male PXR-Humanized Mice.
    Endocrinology. 159 (4): 1595-608.
  13. Lund, H. et al. (2018) Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells.
    Nat Commun. 9 (1): 4845.
  14. Dellinger, A. et al. (2013) Functionalization of gadolinium metallofullerenes for detecting atherosclerotic plaque lesions by cardiovascular magnetic resonance.
    J Cardiovasc Magn Reson. 15: 7.
  15. Zhang, J. et al. (2019) Anti-atherogenic effects of CD36-targeted epigallocatechin gallate-loaded nanoparticles.
    J Control Release. 303: 263-73.
  16. Dhanasekara, C.S. et al. (2021) Nanoparticles target intimal macrophages in atherosclerotic lesions.
    Nanomedicine. 32: 102346.
  17. Liu, J. et al. (2022) Pregnane X Receptor Mediates Atherosclerosis Induced by Dicyclohexyl Phthalate in LDL Receptor-Deficient Mice
    Cells. 11 (7): 1125

Flow Cytometry

Functional Assays

Immunofluorescence

Immunoprecipitation

Western Blotting

Synonyms
GPIV (IIIb)
RRID
AB_2072640
UniProt
Q08857
Entrez Gene
Cd36
GO Terms
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005041 low-density lipoprotein receptor activity
GO:0005794 Golgi apparatus
GO:0008289 lipid binding
GO:0006910 phagocytosis, recognition
GO:0007166 cell surface receptor linked signaling pathway
GO:0008035 high-density lipoprotein particle binding
GO:0050830 defense response to Gram-positive bacterium
GO:0009897 external side of plasma membrane
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010670 positive regulation of oxygen and reactive oxygen species metabolic process
GO:0010671 negative regulation of oxygen and reactive oxygen species metabolic process
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010886 positive regulation of cholesterol storage
GO:0019915 lipid storage
GO:0023048 signal initiation by lipid mediator
GO:0030194 positive regulation of blood coagulation
GO:0030301 cholesterol transport
GO:0032735 positive regulation of interleukin-12 production
GO:0032755 positive regulation of interleukin-6 production
GO:0032760 positive regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0034383 low-density lipoprotein particle clearance
GO:0035634 response to stilbenoid
GO:0042953 lipoprotein transport
GO:0042992 negative regulation of transcription factor import into nucleus
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0043277 apoptotic cell clearance
GO:0043410 positive regulation of MAPKKK cascade
GO:0043619 regulation of transcription from RNA polymerase II promoter in response to oxidative stress
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051636 Gram-negative bacterial cell surface binding
GO:0051637 Gram-positive bacterial cell surface binding
GO:0060100 positive regulation of phagocytosis, engulfment
GO:0060907 positive regulation of macrophage cytokine production
GO:0070892 lipoteichoic acid receptor activity
GO:0071221 cellular response to bacterial lipopeptide
GO:0071222 cellular response to lipopolysaccharide
GO:0071223 cellular response to lipoteichoic acid
GO:0071447 cellular response to hydroperoxide
GO:2000121 regulation of removal of superoxide radicals
View more products with CD36 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products