Chlamydia LPS Antibody | CF 6J12

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Chlamydia LPS Antibody | CF 6J12 gallery image 1

Published customer image:
Mouse anti Chlamydia LPS antibody, clone CF 6J12 used for the detection of Chlamydia LPS in aortic lesions of Chlamydia infected mive by immunofluorescence.
Image caption:
1a. Agarose gel electrophoresis of PCR products (first stage, lanes 2–6; second stage, lanes 8–12). Lanes 2 and 3 using genomic DNA as a template from two Apobtm2SgyLdlrtm1Her/J mice infected with Cpn bacteria; lanes 5 and 6 using genomic DNA from two non-infected Apobtm2SgyLdlrtm1Her/J mice; lanes 8, 9, 11 and 12 using first-stage PCR products 2, 3, 5 and 6 as a template, respectively. Lanes 1 and 7 show the Phi174 DNA/HaeIII maker (Promega). 1b. Chlamydia LPS antibody (MCA 2718, Bio-Rad) stained lesion sites in aortic sinus. Chlamydia LPS antibody (MCA 2718, Bio-Rad) was used as the first antibody (10 mg/ml), anti-mouse IgG-FITC, developed in sheep, was used as a second antibody. Green represents Chlamydia LPS and blue represents cell nuclei stained with 4',6-diamidino-2-phenylindole (DAPI) (Vector Lab, Peterborogh, UK) [N = 6 mice].

From: Xia M, Chen D, Endresz V, Faludi I, Szabo A, Gonczol E, et al. (2013) Immunization of Chlamydia pneumoniae (Cpn)-Infected Apobtm2SgyLdlrtm1Her/J Mice with a Combined Peptide of Cpn Significantly Reduces Atherosclerotic Lesion Development. PLoS ONE 8(12): e81056.

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  • Mouse anti Chlamydia LPS
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    CF 6J12
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2718E, IF, WBdatasheet pdfdatasheet pdf1 mg
    MCA2718
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Chlamydia LPS antibody, clone CF 6J12 recognizes a genus specific epitope within Chlamydia lipopolysaccharide (LPS).

      LPS is a common feature of the outer envelope of gram negative bacteria, which acts as a potent endotoxin, triggering an innate immune response. Studies have shown however, that whilst the LPS of Chlamydia trachomatis does evoke an immune response, it displays only weak endotoxic activity when compared to that of other bacteria such as Salmonella minnesota or Neisseria gonorrhoeae.
    • Intended Use
    • Target Species
      Bacterial
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Elementary bodies from C. trachomatis strain SAF2.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Fusion Partners
      Spleen cells from immunised BALB/c mice were fused with cells of the NS0/U mouse myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Chlamydia LPS Antibody Formats

    Formats Clone Applications Sizes available
    Chlamydia LPS Antibody : Purified CF 6J12 E, IF, WB 1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

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              References

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