Adalimumab Antibody | AbD18654

Adalimumab Antibody | AbD18654 gallery image 1

HCA202 specificity ELISA using various antigens as coating reagents. Human Anti-Adalimumab Antibody (HCA202) was used as the primary antibody followed by Mouse Anti-Penta Histidine tag:HRP Antibody (MCA5995P) as a detection reagent.

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HCA202 specificity titration ELISA. Various antigens (black: Adalimumab, red: Infliximab, green: Alemtuzumab, blue: human IgG1/kappa, light blue: BSA) were used as coating reagents followed by Human Anti-Adalimumab Antibody (HCA202) at increasing concentrations. Mouse Anti-Penta Histidine tag:HRP Antibody (MCA5995P) as a detection reagent.

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HCA202 sensitivity ELISA. Human Anti-Adalimumab Antibody (HCA202) was used as the coating antibody followed by adalimumab at the given concentrations as the antigen. Detection was performed using Mouse Anti-Human Fc specific:HRP Antibody (MCA647P).

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HCA202 PK assay - bridging format. Human Anti-Adalimumab Antibody (HCA202) was used as the coating antibody followed by adalimumab spiked into 10% Human serum as the antigen. Detection was performed by adding HRP conjugated Human Anti-Adalimumab Antibody (HCA204).

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Inhibition of adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human Anti-Adalimumab Antibody (HCA202) titrated into a constant amount of adalimumab. Free adalimumab still capable of binding to the plate was detected using Goat Anti-Human Fab Antibody (STAR126P)

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  • Human anti Adalimumab
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    Anti-adalimumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assay development to measure levels of adalimumab or biosimilars. It is recommended as capture antibody in PK bridging ELISA with HCA204/HCA204P.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA202Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti Adalimumab, clone AbD18654, is a high affinity anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug adalimumab and inhibits it binding to its target, TNFα. The antibody can be used to measure the levels of adalimumab and biosimilar products in bioanalytical assays.

        Clone AbD18654 is recommended as a capture antibody in a pharmacokinetic (PK) bridging assay when used in combination with HRP-conjugated detection antibody HCA204P. The assay will measure free drug, as both antibodies are inhibitory.

        Adalimumab (branded as Humira®) is a fully human monoclonal antibody (IgG1/kappa) approved for the treatment of ankylosing spondylitis, Crohn’s disease, psoriasis and several forms of arthritis. The antibody is specific for TNFα, and acts by blocking the activation of TNF receptors by TNFα. This inactivation down-regulates the inflammatory reactions associated with autoimmune diseases.

        View a summary of all anti-adalimumab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD=0.15 nM by real time, label-free molecular interaction analysis on immobilized adalimumab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        Humira is a registered trademark of Abbott Laboratories.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in an indirect ELISA or in an ELISA bridging assay as a capture antibody together with HCA204P as the detection reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Adalimumab Antibody Formats

      Formats Clone Applications Sizes available
      Adalimumab Antibody : Purified AbD18654 E 0.1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mouse anti Histidine Tag:Alk.Phos.MCA1396A0.1 mgE, WB
        Goat anti Histidine Tag:BiotinAHP1656B0.1 mgE, WB
        Mouse anti Histidine Tag:BiotinMCA1396B0.1 mgE, WB
        Rabbit anti DYKDDDDK Tag:HRPAHP1074P0.1 mgE, WB
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Adalimumab:HRPHCA204P0.1 mgE
            Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC*, E
            Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            Human anti AdalimumabHCA2040.1 mgE
            Hispec Assay DiluentBUF049A50 mlIY
            Recombinant Human TNF AlphaPHP05150 µgE, FN, WB

            Recommended Positive Controls

              Histology Controls

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