Human Anti-Rituximab Antibody bridging ELISA for Pharmacokinetic (PK) assay developmentEnlarge
A microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 (MCA2260P) at a concentration of 3 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.