Rituximab Antibody | MB2A4

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Detection of Rituximab spiked into 10% human serum by sandwich ELISA. Human anti Rituximab (HCA186) was used as the coating antibody and detection was performed by adding HRP conjugated anti-Rituximab antibody MCA2260. Data are shown as the mean of three independent measurements

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Detection of Rituximab in direct ELISA. Rituximab was used as the coating reagent followed by Rat anti Rituximab:HRP (MCA2260P) as the detection antibody

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  • Rat anti Rituximab:FITC
  • Rat anti Rituximab
  • Rat anti Rituximab:HRP
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    Rat anti-rituximab antibody, clone MB2A4, recognizes the monoclonal antibody rituximab, which targets CD20. This anti-rituximab antibody is highly specific and ideal for bioanalytical assay development (PK, PD, ADA assays) in preclinical R&D and clinical trials.
    • Product Type
      Monoclonal Antibody
    • Clone
      MB2A4
    • Isotype
      IgG2a
    3 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2260FFdatasheet pdfdatasheet pdf0.1 mg
      MCA2260F
      MCA2260PEdatasheet pdfdatasheet pdf0.1 mg
      MCA2260P
      MCA2260E, Fdatasheet pdfdatasheet pdf0.2 mg
      MCA2260
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti rituximab antibody, clone MB2A4, specifically recognizes the idiotypic determinants expressed by the rituximab chimeric monoclonal antibody. Rituximab is a therapeutic reagent directed against the human CD20 antigen expressed on the surface of B-cells.

        Rat anti rituximab antibody, clone MB2A4, is specific for rituximab and does not recognize other CD20 antibodies. Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (Cragg et al. 2004 and Hampson et al. 2010). Clone MB2A4 has been used to detect rituximab bound to the surface of the Raji B cell line, however detection of rituximab bound in vivo to B-CLL cells has not been demonstrated. It is possible that complement deposition on rituximab opsonised cells inhibits binding of the anti-rituximab antibody to cell bound rituximab (Beum et al. 2004).
      • Intended Use
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        0.01% Thiomersal
        HRP Stabiliser (BUF052A)
      • Immunogen
        F(ab)2 fragment of Rituximab.
      • Purity
      • Approx. Protein Concentrations
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        TRIS buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line.
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.
        DO NOT FREEZE.
        This product should be stored undiluted.
        Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • Acknowledgements
        Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
        MabThera ® is a registered trademark of Roche in Europe.
        Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
        MabThera ® is a registered trademark of Roche in Europe
        Rituxan® is a registered trademark of Biogen Idec/Genentech in the USA.
        MabThera ® is a registered trademark of Roche in Europe
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        ELISA5 ug/ml as coating antibody
        Flow Cytometry50 ug/ml
      • Application NameYesNoMin DilutionMax Dilution
        ELISA1/200001/200000

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cells in 100ul.
      • ELISA
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent, when conjugated to HRP, in a sandwich ELISA together with HCA186 as the capture reagent. Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with HCA186 as the capture reagent. Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : FITC MB2A4 F 0.1 mg
      Rituximab Antibody : HRP MB2A4 E 0.1 mg
      Rituximab Antibody : Purified MB2A4 E, F 0.2 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Human anti RituximabHCA1860.1 mgE
            HCA186
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Human anti RituximabHCA1860.1 mgE
            HCA186
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Human anti RituximabHCA1860.1 mgE
            HCA186

            Recommended Positive Controls

              Histology Controls

                References

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