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Rituximab Antibody | AbD18423

Rituximab Antibody | AbD18423 gallery image 1

HCA186 PK assay. Human anti Rituximab (HCA186) was used as the coating antibody with Rituximab spiked in human serum as the antigen followed by detection with HRP conjugated anti-Rituximab antibody MCA2260

Rituximab Antibody | AbD18423 gallery image 2

HCA186 specificity ELISA using various antigens as coating reagents. Human anti Rituximab (HCA186) was used as the primary antibody followed by an anti His tag: HRP as a detection reagent

  • Rituximab Antibody | AbD18423 thumbnail image 1
  • Rituximab Antibody | AbD18423 thumbnail image 2
  • Human anti Rituximab
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    Anti-rituximab antibody is a recombinant, neutralizing, high affinity anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It is recommended as capture antibody in PK bridging ELISA format with MCA2260P for detection.
    • Product Type
      Monoclonal Antibody
    • Clone
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA186Edatasheet pdfdatasheet pdf0.1 mg
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      • Human anti rituximab antibody, clone AbD18423 specifically recognises the idiotypic determinants expressed by the rituximab humanised monoclonal antibody, a therapeutic reagent directed against the human CD20 cell surface antigen.

        Human anti rituximab is an anti-idiotypic antibody that specifically recognises the rituximab humanised monoclonal antibody and can be used to measure the level of rituximab in serum from patients. This antibody may be used in a bridging ELISA in conjunction with MCA2260 .

        Additional antibodies recognising rituximab are also available from Bio-Rad.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography.
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
      • Purity
      • Affinity
        The intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
      • Approx. Protein Concentrations
        Total protein concentration 0.5 mg/ml.
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch.
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : Purified AbD18423 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            Human anti Rituximab:HRPHCA061P0.1 mgE
            Human anti Rituximab:HRPHCA062P0.1 mgE
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
            Human anti RituximabHCA0610.1 mgE
            Human anti RituximabHCA0620.1 mgE
            Rat anti RituximabMCA22600.2 mgE, F
            Human anti RituximabHCA061A50 µgE
            Human anti RituximabHCA062A50 µgE

            Recommended Positive Controls

              Histology Controls

                  • Product Images

                  • Rituximab Antibody | AbD18423 thumbnail image 1
                  • Rituximab Antibody | AbD18423 thumbnail image 2


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