Rituximab Antibody | AbD18423

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Rituximab Antibody | AbD18423 gallery image 1

Human Anti-Rituximab Antibody specificity ELISA

A microtiter plate was coated overnight with various antigens at a concentration of 5 µg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) was added at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A). Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) and QuantaBlu Fluorogenic Peroxidase Substrate.

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Human Anti-Rituximab Antibody bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 (MCA2260P) at a concentration of 3 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.

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Inhibition of rituximab binding to CD20 by Human Anti-Rituximab Antibody

Figure A. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.

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  • Human anti Rituximab
  • Human anti Rituximab
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-rituximab antibody is a recombinant anti-idiotypic antibody in monovalent Fab format; it is ideal for bioanalytical assays to measure levels of rituximab or biosimilars. It is recommended as capture antibody in PK bridging ELISA format with MCA2260P for detection.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD18423
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA186Edatasheet pdfdatasheet pdf0.1 mg
      HCA186
      HCA186GEdatasheet pdfdatasheet pdf1 mg
      HCA186G
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human Anti-Rituximab Antibody, clone AbD18423 is a recombinant, anti-idiotypic antibody in monovalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays.

        This antibody is available in two pack sizes, which have different concentrations, 0.1 mg at concentration 0.5 mg/ml, and 1 mg at 1.0 mg/ml.

        This antibody may be used as the capture antibody in a pharmacokinetic bridging ELISA in conjunction with MCA2260 as the detection antibody.

        Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells.View a summary of all anti-rituximab antibodies.
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
        A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography.
        Metal chelate affinity chromatography.
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Rituximab
      • Purity
      • Affinity
        The intrinsic affinity of this antibody was measured as KD=0.13 nM by real time, label-free molecular interaction analysis on immobilized rituximab.
      • Approx. Protein Concentrations
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
        Pack Size: 0.1 mgAntibody concentration 0.5 mg/ml
        Pack Size: 1 mgAntibody concentration 1.0 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        His-tag is a registered trademark of EMD Biosciences.
        Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
        His-tag is a registered trademark of EMD Biosciences.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
      • ELISA
        This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Rituximab Antibody Formats

      Formats Clone Applications Sizes available
      Rituximab Antibody : Purified AbD18423 E 0.1 mg | 1 mg
      • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            MCA2260P
            Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
            MCA5995P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Rat anti Rituximab:FITCMCA2260F0.1 mgF
            MCA2260F
            Human anti Rituximab:HRPHCA061P0.1 mgE
            HCA061P
            Human anti Rituximab:HRPHCA062P0.1 mgE
            HCA062P
            Rat anti Rituximab:HRPMCA2260P0.1 mgE
            MCA2260P
            Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
            MCA5995P
            Human anti RituximabHCA0610.1 mgE
            HCA061
            Human anti RituximabHCA0620.1 mgE
            HCA062
            Rat anti RituximabMCA22600.2 mgE, F
            MCA2260

            Recommended Positive Controls

              Histology Controls

                References

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