Rituximab antibody | AbD18423
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST+5% BSA, Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) was added at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A). Detection was performed using Mouse Anti-Penta Histidine Tag:HRP Antibody (MCA5995P) and QuantaBlu Fluorogenic Peroxidase Substrate.
Figure A. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 1:1. Figure B. PE conjugated rituximab pre-incubated for 1h with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) (red) or Human Fab-FH isotype control (HCA045) (grey) in ratio 5:1 (Anti-Rituximab or isotype:rituximab). All experiments performed on Daudi cell line expressing CD20 and incubated for 30 min with the antibody mix.
A microtiter plate was coated overnight with Human Anti-Rituximab Antibody, clone AbD18423 (HCA186) at a concentration of 1 µg/ml. After washing and blocking with PBST+5% BSA, 10% Human Serum was added spiked with increasing concentrations of rituximab. Detection was performed using Rat Anti-Rituximab:HRP Antibody, clone MB2A4 (MCA2260P) at a concentration of 3 µg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three independent measurements.
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E FN ResetHuman anti Rituximab
- Product Type
- Monoclonal Antibody
- Clone
- AbD18423
- Isotype
- HuCAL Fab monovalent
- Specificity
- Rituximab
Antibody Quality and Performance Guarantee
| Human Anti-Rituximab Antibody, clone AbD18423 is a recombinant, anti-idiotypic antibody in monovalent Fab format that specifically recognizes the monoclonal antibody drug rituximab and inhibits it binding to its target, CD20. The antibody can be used to measure the levels of rituximab and biosimilar products in bioanalytical assays. This antibody may be used as the capture antibody in a pharmacokinetic bridging ELISA in conjunction with MCA2260 as the detection antibody. Rituximab (reference product branded as Rituxan) is a chimeric mouse/human monoclonal antibody approved for the treatment of certain autoimmune diseases and cancer, including non-Hodgkin's lymphoma, chronic lymphocytic leukemia and rheumatoid arthritis. The antibody is specific for the cell surface protein CD20, which is widely expressed on B cells. Through three different mechanisms of action it eliminates B cells from the body, enabling the development of a new population of healthy B cells. View a summary of all Anti-Rituximab Antibodies. |
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- Product Form
- A monovalent human recombinant Fab (kappa light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- HCA186G: Metal chelate affinity chromatography
- HCA186: Metal chelate affinity chromatography.
- Source
- E.coli
- Buffer Solution
- HCA186G: Phosphate buffered saline
- HCA186: Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Rituximab
- Affinity
- The intrinsic affinity of this antibody is KD=0.13 nM as measured by real time, label-free molecular interaction analysis on immobilized rituximab.
- Approx. Protein Concentrations
- HCA186G: Antibody concentration 1.0 mg/ml
- HCA186: Antibody concentration 0.5 mg/ml.
- Regulatory
- HCA186G: For research purposes only
- HCA186: For research purposes only.
- Guarantee
- HCA186G: 12 months from date of despatch
- HCA186: 12 months from date of despatch.
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used as a capture antibody in a sandwich ELISA together with MCA2260P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Human anti Rituximab | HCA061 | E | 0.1 mg |
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| Description | Human anti Rituximab | ||||||
| Rat anti Rituximab | MCA2260 | E F | 0.2 mg |
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| Description | Rat anti Rituximab | ||||||
| Rat anti Rituximab:FITC | MCA2260F | F | 0.1 mg |
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| Description | Rat anti Rituximab:FITC | ||||||
| Rat anti Rituximab:HRP | MCA2260P | E | 0.1 mg |
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| Description | Rat anti Rituximab:HRP | ||||||
| Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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| Description | Mouse anti Penta Histidine Tag:HRP | ||||||
References for Rituximab antibody
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Komori, M. et al. (2016) Insufficient disease inhibition by intrathecal rituximab in progressive multiple sclerosis.
Ann Clin Transl Neurol. 3 (3): 166-79.
- Synonyms
- Mabthera
- Rituxan
View more products with RITUXIMAB specificity
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