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|The cell proliferation assay reagent alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.
alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
Some variability in the absorbance may occur between batches of alamarBlue® but all batches should fall between 0.84 and 0.95AU when measured between 600nm and 602nm on a spectrophotometer.
Please view the full cell proliferation assay instructions.
Colorimetric and Fluorescence result calculators are available here.
This site includes:
Frequently Asked Questions
- Intended Use
•Cell proliferation assays.
• The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
•Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo. toxicity.
•alamarBlue is for use between pH6.8 and pH7.4.
- Product Form
- Preservative Stabilisers
- None present
- Test Principle
- Cell proliferation assay.
•Growing cells cause a chemical reduction of alamarBlue.
•Continued growth maintains a reduced environment. (fluorescent, red).
•Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation.
•Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
•Absorbance is monitored at 570nm and 600nm.
- For research purposes only
- Guaranteed until date of expiry. Please see product label.
- Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012A.pdf.
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012B.pdf.
References for alamarBlue®
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View The Latest Product References
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