alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.
Some variability in the absorbance may occur between batches of AlamarBlue® but all batches should fall between 0.84 and 0.95AU when measured between 600nm and 602nm on a spectrophotometer.
Full cell proliferation assay instructions can be found here
Colorimetric and Fluorescence result calculators can be found here.
For further information and Technical help about alamarBlue®, the cell proliferation assay reagent, please visit www.bio-rad-antibodies.com/alamarBlue
This site includes:
Frequently Asked Questions
- Intended Use
• Cell proliferation assays.
• The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
• Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
• alamarBlue® is for use between pH6.8 and pH7.4.
- Product Form
- Preservative Stabilisers
- None present
- Test Principle
- Cell proliferation assay.
• Growing cells cause a chemical reduction of alamarBlue®.
• Continued growth maintains a reduced environment. (fluorescent, red).
• Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation.
• Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
• Absorbance is monitored at 570nm and 600nm.
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
- Guaranteed until date of expiry. Please see product label.
- Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
- For research purposes only
Applications of alamarBlue®
|Application Name||Verified||Min Dilution||Max Dilution|
- Instructions For Use
- Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012A.pdf
Useful Reagents Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|alamarBlue®||BUF012B||E FN IF||100 ml|
|alamarBlue®||BUF012A||E FN IF||25 ml|
Product Specific References
References for alamarBlue®
Borg, S.A. et al. (2003) Expression of interleukin-6 and its effects on growth of HP75 human pituitary tumor cells.
J Clin Endocrinol Metab. 88: 4938-44.
Brieger, A. et al. (2002) Transient mismatch repair gene transfection for functional analysis of genetic hMLH1 and hMSH2 variants.
Gut. 51: 677-84.
Cao, S. et al. (2010) Residue histidine 669 is essential for the catalytic activity of Bacillus anthracis lethal factor.
J Bacteriol. 192: 5799-805.
Carroll, J. et al. (2009) Optimization of a rapid viability assay for Mycobacterium avium subsp. paratuberculosis by using alamarBlue.
Appl Environ Microbiol. 75: 7870-2.
Dawson, C.W. et al. (2003) Epstein-Barr virus latent membrane protein 1 (LMP1) activates the phosphatidylinositol 3-kinase/Akt pathway to promote cell survival and induce actin filament remodeling.
J Biol Chem. 278: 3694-704.
Feder-Mengus, C. et al. (2007) Multiple mechanisms underlie defective recognition of melanoma cells cultured in three-dimensional architectures by antigen-specific cytotoxic T lymphocytes.
Br J Cancer. 96: 1072-82.
Ferro, V.A. et al. (2003) In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller.
Antimicrob Agents Chemother. 47: 1137-9.
Garces, A. et al. (2010) EspA acts as a critical mediator of ESX1-dependent virulence in Mycobacterium tuberculosis by affecting bacterial cell wall integrity.
PLoS Pathog. 6(6): e1000957.
Genetos, D.C. et al. (2010) Oxygen tension modulates neurite outgrowth in PC12 cells through a mechanism involving HIF and VEGF.
J Mol Neurosci. 40: 360-6.
Guo, Y. et al. (2002) An antiangiogenic urokinase-derived peptide combined with tamoxifen decreases tumor growth and metastasis in a syngeneic model of breast cancer.
Cancer Res. 62: 4678-84.
Habtemariam, S. et al. (2003) In vitro antileishmanial effects of antibacterial diterpenes from two Ethiopian Premna species: P. schimperi and P. oligotricha.
BMC Pharmacol. 3:6.
Hsu, D.S. et al. (2010) Regulation of excision repair cross-complementation group 1 by Snail contributes to cisplatin resistance in head and neck cancer.
Clin Cancer Res. 16: 4561-71.
Latham, J.P. et al. (2000) Prostate-specific antigen promoter/enhancer driven gene therapy for prostate cancer: construction and testing of a tissue-specific adenovirus vector.
Cancer Res. 60: 334-41.
Mansour, N.R. and Bickle, Q.D. (2010) Comparison of microscopy and Alamar blue reduction in a larval based assay for schistosome drug screening.
PLoS Negl Trop Dis. 4: e795.
McCormick, A.L. et al. (2001) Immunization with an interferon-gamma-gp120 fusion protein induces enhanced immune responses to human immunodeficiency virus gp120.
J Infect Dis. 184: 1423-30.
Misra, S.K. et al. (2010) Effect of nanoparticulate bioactive glass particles on bioactivity and cytocompatibility of poly(3-hydroxybutyrate) composites.
J R Soc Interface. 7: 453-65.
Naughton, P. et al. (2002) Induction of heme oxygenase 1 by nitrosative stress. A role for nitroxyl anion.
J Biol Chem. 277: 40666-74.
Prakash, J. et al. (2006) Intracellular delivery of the p38 mitogen-activated protein kinase inhibitor SB202190 [4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)1H-imidazole] in renal tubular cells: a novel strategy to treat renal fibrosis.
J Pharmacol Exp Ther. 319: 8-19.
Roghanian, A. et al. (2006) Inflammatory lung secretions inhibit dendritic cell maturation and function via neutrophil elastase.
Am J Respir Crit Care Med. 174: 1189-98.
Sagar, J. et al. (2010) Lowering the apoptotic threshold in colorectal cancer cells by targeting mitochondria.
Cancer Cell Int. 10: 31.
Sakurai, T. and Fujiwara, K. (2001) Modulation of cell adhesion and viability of cultured murine bone marrow cells by arsenobetaine, a major organic arsenic compound in marine animals.
Br J Pharmacol. 132: 143-50.
Scapagnini, G. et al. (2002) Caffeic acid phenethyl ester and curcumin: a novel class of heme oxygenase-1 inducers.
Mol Pharmacol. 61: 554-61.
Tao, L. and Yu, J.H. (2011) AbaA and WetA govern distinct stages of Aspergillus fumigatus development.
Microbiology. 157: 313-26.
Tiwari, A. et al. (2002) Development of a hybrid cardiovascular graft using a tissue engineering approach.
FASEB J. 16: 791-6.
Golay, J. et al. (2010) Possible misinterpretation of the mode of action of therapeutic antibodies in vitro: homotypic adhesion and flow cytometry result in artefactual direct cell death
Goren, A. et al. (2010) Encapsulated human mesenchymal stem cells: a unique hypoimmunogenic platform for long-term cellular therapy.
FASEB J. 24: 22-31.
Landgraf, K.E. et al. (2010) Allosteric peptide activators of pro-hepatocyte growth factor stimulate Met signaling.
J Biol Chem. 285: 40362-72.
Lee, do Y. et al. (2010) Activation of PERK signaling attenuates Abeta-mediated ER stress.
PLoS One. 5: e10489.
Scotter, E.L. et al. (2010) Neuroprotective potential of CB1 receptor agonists in an in vitro model of Huntington's disease.
Br J Pharmacol. 160: 747-61.
Wu, G. et al. (2010) A chimeric protein that functions as both an anthrax dual-target antitoxin and a trivalent vaccine.
Antimicrob Agents Chemother. 54: 4750-7.
Xiao, P. et al. (2010) Aspergillus fumigatus flbB encodes two basic leucine zipper domain (bZIP) proteins required for proper asexual development and gliotoxin production.
Eukaryot Cell. 9: 1711-23.
Suter, S.E. et al. (2011) FLT3 mutations in canine acute lymphocytic leukemia.
BMC Cancer. 11: 38.
Goetzenich, A. et al. (2011) The effects of metoprolol on hypoxia- and isoflurane-induced cardiac late-phase preconditioning.
Acta Anaesthesiol Scand. 55 (7): 862-9.
Lewis, C.S. et al. (2010) Local Antibiotic Delivery with Bovine Cancellous Chips.
J Biomater Appl. 26: 491-506.
Alsford, S. and Horn, D. (2011) Elongator Protein 3b Negatively Regulates Ribosomal DNA Transcription in African Trypanosomes.
Mol Cell Biol.31: 1822-32.
Crilly, A. et al. (2011) Phosphodiesterase 4 (PDE4) regulation of proinflammatory cytokine and chemokine release from rheumatoid synovial membrane.
Ann Rheum Dis. 70: 1130-7.
Paget, C. et al. (2011) Potential Role of Invariant NKT Cells in the Control of Pulmonary Inflammation and CD8+ T Cell Response during Acute Influenza A Virus H3N2 Pneumonia.
J Immunol. 186: 5590-602.
Lakhkar, N. et al. (2011) Titanium and strontium-doped phosphate glasses as vehicles for strontium ion delivery to cells.
J Biomater Appl. 25: 877-93.
Wilson, B.A. et al. (2011) High-throughput screen identifies novel inhibitors of cancer biomarker α-methylacyl coenzyme A racemase (AMACR/P504S).
Mol Cancer Ther. 10: 825-38.
Lau, L.I. et al. (2011) The Effect of Photooxidative Stress and Inflammatory Cytokine on Complement Factor H Expression in Retinal Pigment Epithelial Cells.
Invest Ophthalmol Vis Sci. 52: 6832-41.
Arlian, B.M. and Tinker, J.K. (2011) Mucosal Immunization with a Staphylococcus aureus IsdA-Cholera Toxin A2/B Chimera Induces Antigen-Specific Th2-Type Responses in Mice.
Clin Vaccine Immunol. 18: 1543-51.
Voloshin, T. et al. (2011) G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist.
Blood. 118: 3426-35.
Rao, T.D. et al. (2011) Dual-Fluorescence Isogenic High-Content Screening for MUC16/CA125 Selective Agents.
Mol Cancer Ther. 10: 1939-48.
Uitdehaag, J.C. et al. (2011) Multidimensional Profiling of CSF1R Screening Hits and Inhibitors: Assessing Cellular Activity, Target Residence Time, and Selectivity in a Higher Throughput Way.
J Biomol Screen. 16: 1007-17.
Rzhepishevska, O. et al (2011) The antibacterial activity of ga3+ is influenced by ligand complexation as well as the bacterial carbon source.
Antimicrob Agents Chemother. 55: 5568-80.
Xu, S. et al. (2011) Marek's disease virus type 1 microRNA miR-M3 suppresses cisplatin-induced apoptosis by targeting Smad2 of the transforming growth factor beta signal pathway.
J Virol. 2011 Jan;85(1):276-85.
Ardakani, A.G. et al. (2014) Quantifying the correlation between spatially defined oxygen gradients and cell fate in an engineered three-dimensional culture model.
J R Soc Interface. 11. pii: 20140501.
Nakayama, G.R. et al. (1997) Assessment of the Alamar Blue assay for cellular growth and viability in vitro.
J Immunol Methods. 204: 205-8.
Diril, M.K. et al. (2012) Cyclin-dependent kinase 1 (Cdk1) is essential for cell division and suppression of DNA re-replication but not for liver regeneration.
Proc Natl Acad Sci U S A. 109: 3826-31.
Warrier, T. et al. (2012) Antigen 85C inhibition restricts Mycobacterium tuberculosis growth through disruption of cord factor biosynthesis.
Antimicrob Agents Chemother. 56: 1735-43.
Dreidax, D. et al. (2013) Low p14ARF expression in neuroblastoma cells is associated with repressed histone mark status, and enforced expression induces growth arrest and apoptosis.
Hum Mol Genet. 22: 1735-45.
Wang, H. et al. (2014) Enhanced osteoblast responses to poly ether ether ketone surface modified by water plasma immersion ion implantation.
Colloids Surf B Biointerfaces. 117: 89-97.
Park, K.H. et al. (2014) Expression of polysialylated neural cell adhesion molecules on adult stem cells after neuronal differentiation of inner ear spiral ganglion neurons.
Biochem Biophys Res Commun. pii: S0006-291X(14)00889-4.
Moreira, Â et al. (2015) Adipocyte secreted factors enhance aggressiveness of prostate carcinoma cells.
PLoS One. 10 (4): e0123217.
Rao, T.D. et al. (2015) Expression of the Carboxy-Terminal Portion of MUC16/CA125 Induces Transformation and Tumor Invasion.
PLoS One. 10 (5): e0126633.
Mabuchi, Y. & Frankel, T.L. (2016) Functions of innate and acquired immune system are reduced in domestic pigeons (Columba livia domestica) given a low protein diet
Royal Society Open Science. 3 (3): 150408.
Santofimia-Castaño Patricia et al. (2014) Change in the Characteristics of Ca2+Signaling in Pancreatic Acinar Cells in Culture
The Open Access Journal of Science and Technology. 2: 1-12.
Xu, S. et al. (2016) MicroRNA-33 promotes the replicative senescence of mouse embryonic fibroblasts by suppressing CDK6
Biochem Biophys Res Com. Apr 6 [Epub ahead of print]
Drong, C. et al. (2016) Effects of monensin and essential oils on immunological, haematological and biochemical parameters of cows during the transition period.
J Anim Physiol Anim Nutr (Berl). Mar 2. [Epub ahead of print]
van Driel, P.B. et al. (2016) EGFR targeted nanobody-photosensitizer conjugates for photodynamic therapy in a pre-clinical model of head and neck cancer.
J Control Release. 229: 93-105.
Howe GA et al. (2016) Focal Adhesion Kinase Inhibitors in Combination with Erlotinib Demonstrate Enhanced Anti-Tumor Activity in Non-Small Cell Lung Cancer.
PLoS One. 11 (3): e0150567.
Fernández-Gutiérrez M et al. (2016) Stimuli-responsive chitosan/poly (N-isopropylacrylamide) semi-interpenetrating polymer networks: effect of pH and temperature on their rheological and swelling properties.
J Mater Sci Mater Med. 27 (6): 109.
van Driel, P.B. et al. (2016) EGFR targeted nanobody-photosensitizer conjugates for photodynamic therapy in a pre-clinical model of head and neck cancer.
J Control Release. 229: 93-105.
Grasys J et al. (2016) Content of Soluble Factors and Characteristics of Stromal Vascular Fraction Cells in Lipoaspirates from Different Subcutaneous Adipose Tissue Depots.
Aesthet Surg J. Feb 23. pii: sjw022. [Epub ahead of print]
Liu, T.T. et al. (2016) Effects of processing methods on composition and functionality of volatile components isolated from immature fruits of atemoya.
Food Chem. 202: 176-83.
Tuin, S.A. et al. (2016) Creating tissues from textiles: scalable nonwoven manufacturing techniques for fabrication of tissue engineering scaffolds.
Biomed Mater. 11 (1): 015017.
Chauhan, S. et al. (2016) Cdk2 catalytic activity is essential for meiotic cell division in vivo.
Biochem J. 473 (18): 2783-98.
Yu, Hui-Chieh. et al. (2016) Effects of fibroblast growth factor-2 on cell proliferation of cementoblasts
Journal of Dental Sciences. Nov 5 [Epub ahead of print]
Bertoldi, S. et al. (2015) Exploiting novel sterilization techniques for porous polyurethane scaffolds.
J Mater Sci Mater Med. 26 (5): 182.
Botero A et al. (2016) In vitro drug susceptibility of two strains of the wildlife trypanosome, Trypanosoma copemani: A comparison with Trypanosoma cruzi.
Int J Parasitol Drugs Drug Resist. 7 (1): 34-41.
Zhang, Y-G, et al. (2016) Novel interconnected nanochannel hydroxyapatite ceramic: in situ synthesis, microstructure, and permeability
Ceramics International. Dec 23 [Epub ahead of print]
Bernardini, F.P. et al. (2015) Superficial Enhanced Fluid Fat Injection (SEFFI) to Correct Volume Defects and Skin Aging of the Face and Periocular Region.
Aesthet Surg J. 35 (5): 504-15.
Howe, G.A. et al. (2016) Focal Adhesion Kinase Inhibitors in Combination with Erlotinib Demonstrate Enhanced Anti-Tumor Activity in Non-Small Cell Lung Cancer.
PLoS One. 11 (3): e0150567.
Li, G. et al. (2017) Antimicrobial susceptibility and MIC distribution of 41 drugs against clinical isolates from China and reference strains of nontuberculous mycobacteria.
Int J Antimicrob Agents. 49 (3): 364-374.
Deschamps, E. et al. (2014) Characterization of indoor dust from Brazil and evaluation of the cytotoxicity in A549 lung cells.
Environ Geochem Health. 36 (2): 225-33.
Lara, D. et al. (2010) Anti-trypanosomatid activity of ceragenins.
J Parasitol. 96 (3): 638-42.
Lee, Y.W. et al. (2017) Effects of Redox Modulation on Cell Proliferation, Viability, and Migration in Cultured Rat and Human Tendon Progenitor Cells.
Oxid Med Cell Longev. 2017: 8785042.
DiSpigna, G. et al. (2017) Human cardiac multipotent adult stem cells in 3D matrix: new approach of tissue engineering in cardiac regeneration post-infarction.
J Biol Regul Homeost Agents. 31 (4): 911-921.
Martínez-morcillo, S. et al. (2018) The organophosphorus pesticide dimethoate decreases cell viability and induces changes in different biochemical parameters of rat pancreatic stellate cells.
Toxicol In Vitro. 54: 89-97.
Jyotsana, N. et al. (2019) Lipid nanoparticle-mediated siRNA delivery for safe targeting of human CML in vivo.
Ann Hematol. 98 (8): 1905-18.
Looi, C.Y. et al. (2013) Induction of apoptosis in human breast cancer cells via caspase pathway by vernodalin isolated from Centratherum anthelminticum (L.) seeds.
PLoS One. 8 (2): e56643.
Shechter, D. et al. (2020) Breast Cancer-Derived Microparticles Reduce Cancer Cell Adhesion, an Effect Augmented by Chemotherapy.
Cells. 9 (10) 2269.
Jiménez-Holguín, J. et al. (2020) Strontium-Modified Scaffolds Based on Mesoporous Bioactive Glasses/Polyvinyl Alcohol Composites for Bone Regeneration.
Materials (Basel). 13 (23) Dec 03 [Epub ahead of print].
Perut, F. et al. (2020) Citrate Supplementation Restores the Impaired Mineralisation Resulting from the Acidic Microenvironment: An In Vitro Study.
Nutrients. 12 (12)Dec 09 [Epub ahead of print].
Huber, L. et al. (2020) FGF Expression in HPV16-positive and -negative SCC After Treatment With Small-molecule Tyrosine Kinase Inhibitors and Everolimus.
Anticancer Res. 40 (10): 5621-30.
Ansari, Z. et al. (2020) In-Situ Synthesis and Characterization of Chitosan/Hydroxyapatite Nanocomposite Coatings to Improve the Bioactive Properties of Ti6Al4V Substrates.
Materials (Basel). 13 (17)Aug 26 [Epub ahead of print].
Yang, P.M. et al. (2020) Carbon monoxide‑releasing molecules protect against blue light exposure and inflammation in retinal pigment epithelial cells.
Int J Mol Med. 46 (3): 1096-106.
Calejo, I. et al. (2019) A Textile Platform Using Continuous Aligned and Textured Composite Microfibers to Engineer Tendon-to-Bone Interface Gradient Scaffolds.
Adv Healthc Mater. 8 (15): e1900200.
Huang, C.L. et al. (2020) Antimicrobial Activity of Electrospun Polyvinyl Alcohol Nanofibers Filled with Poly[2-(tert-butylaminoethyl) Methacrylate]-Grafted Graphene Oxide Nanosheets.
Polymers (Basel). 12 (7)Jun 28 [Epub ahead of print].
Wu, L. et al. (2020) Human airway-like multilayered tissue on 3D-TIPS printed thermoresponsive elastomer/collagen hybrid scaffolds.
Acta Biomater. 113: 177-95.
Cometa, S. et al. (2021) A 3D Printed Composite Scaffold Loaded with Clodronate to Regenerate Osteoporotic Bone: In Vitro Characterization.
Polymers (Basel). 13(1):150.
Badwelan, M. et al. (2020) Poly(δ-valerolactone)/Poly(ethylene-co-vinylalcohol)/β-Tri-calcium Phosphate Composite as Scaffolds: Preparation, Properties, and In Vitro Amoxicillin Release.
Polymers (Basel). 13(1):46.
Alhuthali, H.M. et al. (2020) The natural alkaloid Jerantinine B has activity in acute myeloid leukemia cells through a mechanism involving c-Jun.
BMC Cancer. 20 (1): 629.
Ho, C.L. et al. (2020) Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
BMC Complement Med Ther. 20 (1): 200.
Fatima, S. et al. (2021) Selenium Nanoparticles by Moderating Oxidative Stress Promote Differentiation of Mesenchymal Stem Cells to Osteoblasts.
Int J Nanomedicine. 16: 331-43.
Mojena-Medina, D. et al. (2020) Design, Implementation, and Validation of a Piezoelectric Device to Study the Effects of Dynamic Mechanical Stimulation on Cell Proliferation, Migration and Morphology.
Sensors (Basel). 20 (7): 2155.
Gonzalez, A. et al. (2020) Melatonin modulates red-ox state and decreases viability of rat pancreatic stellate cells.
Sci Rep. 10 (1): 6352.
Juillerat-Jeanneret, L. et al. (2008) Heterogeneity of human glioblastoma: glutathione-S-transferase and methylguanine-methyltransferase.
Cancer Invest. 26 (6): 597-609.
Scialla, S. et al. (2019) Bioactive chitosan-based scaffolds with improved properties induced by dextran-grafted nano-maghemite and l-arginine amino acid.
J Biomed Mater Res A. 107 (6): 1244-52.
Almeshari, A. et al. (2021) The additive effect of iloprost on the biological properties of Mineral trioxide aggregate on mesenchymal stem cells
J Dental Sci. 11 May [Epub ahead of print].
Pagani, S. et al. (2021) Mechanical and in vitro. biological properties of uniform and graded Cobalt-chrome lattice structures in orthopedic implants.
J Biomed Mater Res B Appl Biomater. May 08 [Epub ahead of print].
Pacheco, P.A.F. et al. (2020) Synthesis of new N,S-acetal analogs derived from juglone with cytotoxic activity against Trypanosoma cruzi..
J Bioenerg Biomembr. 52 (3): 199-213.
Gloria, A. et al. (2020) Customised multiphasic nucleus/annulus scaffold for intervertebral disc repair/regeneration.
Connect Tissue Res. 61 (2): 152-62.
Imieje, V.O. et al. (2021) Antileishmanial Derivatives of Humulene from Asteriscus hierochunticus. with in silico. Tubulin Inhibition Potential
Records of Natural Products. (2): 150-71.
Liu, Z. et al. (2021) Distinct BTK inhibitors differentially induce apoptosis but similarly suppress chemotaxis and lipid accumulation in mantle cell lymphoma.
BMC Cancer. 21 (1): 732.
Estaras, M. et al. (2020) Melatonin modulates proliferation of pancreatic stellate cells through caspase-3 activation and changes in cyclin A and D expression.
J Physiol Biochem. 76 (2): 345-55.
Rampersad SN (2012) Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.
Sensors (Basel). 12 (9): 12347-60.
Fluorescent SpectraviewerWatch the Tool Tutorial Video ▸
How to Use the SpectraviewerWatch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra