alamarBlue®

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alamarBlue®

Product Type
Accessory Reagent
Product Code Applications Pack Size List Price Quantity
BUF012A
Datasheet
E FN IF
SDS
25 ml loader

BUF012B
Datasheet
E FN IF
SDS
100 ml loader

The cell proliferation assay reagent alamarBlue® is designed to provide a rapid and sensitive measure of cell proliferation and cytotoxicity in various human and animal cell lines, bacteria and fungi.

alamarBlue® is an indicator dye, that incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and changes colour in response to the chemical reduction of growth medium, resulting from cell growth. The alamarBlue® cell proliferation assay reagent is designed to quantitatively measure the proliferation of various human and animal cell lines, bacteria and fungi.

Some variability in the absorbance may occur between batches of AlamarBlue® but all batches should fall between 0.84 and 0.95AU when measured between 600nm and 602nm on a spectrophotometer.

Full cell proliferation assay instructions can be found here

Colorimetric and Fluorescence result calculators can be found here.

For further information and Technical help about alamarBlue®, the cell proliferation assay reagent, please visit www.bio-rad-antibodies.com/alamarBlue
This site includes:
Frequently Asked Questions
Example calculations
Product-related references

Product Details

Intended Use
• Cell proliferation assays.
• The reagent can be used to establish proliferation or relative cytotoxicity in a cell proliferation assay.
• Baseline data for predicting the toxicity of related novel agents can be compared to baseline data with known in-vivo toxicity.
• alamarBlue® is for use between pH6.8 and pH7.4.

Product Form
Liquid
Preservative Stabilisers
None present
Test Principle
Cell proliferation assay.
• Growing cells cause a chemical reduction of alamarBlue®.
• Continued growth maintains a reduced environment. (fluorescent, red).
• Inhibition of growth maintains an oxidized environment. (non-fluorescent, blue).
• Data may be collected using either fluorescence-based or absorbance-based instrumentation.
• Fluorescence is monitored at 530-560nm excitation wavelength and 590nm emission wavelength.
• Absorbance is monitored at 570nm and 600nm.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Acknowledgements
Manufactured for Bio-Rad by Trek Diagnostic System. U.S. patent 5,501,959.
Regulatory
For research purposes only

Applications of alamarBlue®

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/BUF012A.pdf

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
alamarBlue® BUF012B E FN IF 100 ml loader
alamarBlue® BUF012A E FN IF 25 ml loader

Application Based External Images

Cytotoxic Assays

ELISA

FLISA

Immunofluorescence

Product Specific References

References for alamarBlue®

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Further Reading

  1. Rampersad SN (2012) Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays.
    Sensors (Basel). 12 (9): 12347-60.

Fluorescent Spectraviewer

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How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra