Protocol: PK Bridging ELISA Detecting Total Drug using Anti-Etanercept Antibodies
Pharmacokinetic (PK) Bridging ELISA protocol detects total drug (free, partially and fully bound): for use with Anti-Etanercept Antibody products HCA276 and HCA279P
This method provides a procedure for carrying out a PK ELISA with Anti-Etanercept Antibodies, catalog numbers HCA276 (capture antibody) and HCA279P (detection antibody), and using etanercept for the standard curve.
HCA279P Anti-Etanercept Antibody does not inhibit the binding of etanercept to TNFα and therefore this assay can detect total drug (free, partially bound and fully bound). The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
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