Protocol: PK Bridging ELISA Detecting Total Drug Using Anti-Tocilizumab Antibodies
Pharmacokinetic (PK) Bridging ELISA: For use with anti-tocilizumab monoclonal antibody products HCA252 and HCA257P
This method provides a procedure for carrying out a PK ELISA with anti-tocilizumab antibodies, product codes HCA252 (capture antibody) and HCA257P (detection antibody), and using tocilizumab monoclonal antibody for the standard curve.
The HCA257P anti-tocilizumab antibody does not inhibit the binding of tocilizumab to IL-6R and can therefore detect total drug; free, partially bound and fully bound. The method should always be used in conjunction with product and batch specific information provided with each vial (see product datasheets). This protocol will need to be adjusted for use with different detection methods and immunoassay technology platforms.
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