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IgG antibody

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Sheep Anti Rabbit IgG Antibody - RPE thumbnail image 1
Sheep Anti Rabbit IgG Antibody - RPE gallery image 1
Published customer image:
RPE conjugated Sheep anti Rabbit IgG antibody (STAR35) used for the detection of bound rabbit anti mPGES-2 and anti cPGES antibodies by flow cytometry.
Image caption:
Effect of TNFα on protein expression of PGE synthases and COX-2 and production of PGE2. (A) Gingival fibroblasts were treated with TNFα (20 ng/ml) or without TNFα for the indicated time period. Cells were harvested, total protein was extracted and protein expression of the PGE2-synthesizing enzymes mPGES-1, mPGES-2, cPGES and COX-2 as well as the loading control actin was analyzed by western blot. (B) Gingival fibroblasts were stimulated with TNFα (20 ng/ml) or cultured without TNFα (control) for 24 h. Protein expression of mPGES-1, mPGES-2, cPGES and COX-2 was investigated by flow cytometry, using specific antibodies. (C) Gingival fibroblasts were cultured with or without TNFα (20 ng/ml) for the indicated time period. Levels of PGE2 in the culture media were measured by EIA on a Luminex system, and are presented as mean ± s.d. Asterisks (*) indicate a significant difference from untreated cells at the corresponding time point (p < 0.05). The results are representative for all three cell lines and all analyses were performed in triplicates.

From: Båge T, Lindberg J, Lundeberg J, Modéer T, Yucel-Lindberg T.
Signal pathways JNK and NF-kappaB, identified by global gene expression profiling, are involved in regulation of TNFalpha-induced mPGES-1 and COX-2 expression in gingival fibroblasts.
BMC Genomics. 2010 Apr 15;11:241.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Sheep anti Rabbit IgG:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
STAR35A
Datasheet Datasheet Datasheet
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F 1 ml loader
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Sheep anti Rabbit IgG antibody recognizes rabbit IgG.

Target Species
Rabbit
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Rabbit IgG.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgG antibody

  1. Båge, T. et al. (2010) Signal pathways JNK and NF-kappaB, identified by global gene expression profiling, are involved in regulation of TNFalpha-induced mPGES-1 and COX-2 expression in gingival fibroblasts.
    BMC Genomics. 11: 241.
  2. Senthilkumaran, C. et al. (2015) Localization of annexins A1 and A2 in the respiratory tract of healthy calves and those experimentally infected with Mannheimia haemolytica.
    Vet Res. 46: 6.
  3. Williams, K.M. et al. (2022) Prospective Phase II Trial of Montelukast to Treat Bronchiolitis Obliterans Syndrome after Hematopoietic Cell Transplantation and Investigation into Bronchiolitis Obliterans Syndrome Pathogenesis.
    Transplant Cell Ther. S2666-6367(22)00049-5.

RRID
AB_321858
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding

STAR35A

151294 163132

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