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IL-13 antibody

Rabbit anti Mouse Interleukin-13

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-13

Product Code Applications Pack Size List Price Your Price Qty
AAM34
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Mouse Interleukin-13 antibody recognizes mouse Interleukin-13 (IL-13), a 12.3kDa immunoregulatory cytokine produced primarily by activated T helper type 2 (Th2) cells and also by mast cells and NK cells.

The biological properties of IL-13 include stimulation of B cell proliferation and antibody production. IL-13 plays an important role in the induction of the pathophysiological features of allergic asthma, independently of Immunoglobulin E and eosinophils (Wills-Karp et al. 1998).

Target Species
Mouse
Product Form
Purified IgG - lyophilized
Reconstitution
Reconstitute with 0.1ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to mouse IL-13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant mouse IL-13.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5 2.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
ELISA
AAM34 may be used in an indirect ELISA or as a capture reagent in a sandwich ELISA with AAM34B as the detection antibody.

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Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
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Description Goat anti Rabbit IgG (Fc):FITC
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
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Description Goat anti Rabbit IgG (Fc):HRP
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
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Description Goat anti Rabbit IgG (H/L):HRP
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
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Description Sheep anti Rabbit IgG:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Mouse Interleukin-13:Biotin AAM34B E WB 50 µg loader
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Description Rabbit anti Mouse Interleukin-13:Biotin
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB * 0.5 ml
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Description TidyBlot Western Blot Detection Reagent:HRP

References for IL-13 antibody

  1. Wills-karp, M. et al. (1998) Interleukin-13: central mediator of allergic asthma.
    Science. 282 (5397): 2258-61.

RRID
AB_2264676
UniProt
P20109
Entrez Gene
Il13
GO Terms
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0006955 immune response
GO:0043032 positive regulation of macrophage activation

AAM34

140415 161213 163683 165279

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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