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IgG antibody

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Anti Rabbit IgG (Fc) Antibody thumbnail image 1
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Published customer image:
Horseradish peroxidase conjugated Goat anti Rabbit IgG antibody (STAR121P) used as a secondary reagent to detect bound rabbit antibodies in western blotting on human lysates.
Image caption:
(c) Left: Western blot of phosphorylated PI3K and Akt in control and PAM-treated 3D A549 spheroids. Total protein levels of PI3K and Akt were analyzed. β-Actin was used as a loading control. Right: quantification of phosphorylated PI3K and Akt normalized to the total amount of the same proteins. (d) Left: Western blot of phosphorylated ERK, p38, and JNK in control and PAM-treated 3D A549 cells. Total protein levels of ERK, p38, and JNK were analyzed. β-Actin was used as a loading control. Right: quantification of phosphorylated ERK, p38, and JNK normalized to the total amount of the same proteins.

From: Li Y, Lv Y, Zhu Y, Yang X, Lin B, Li M, Zhou Y, Tan Z, Choi EH, Wang J, Wang S, Liu Y.
Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
Oxid Med Cell Longev. 2022 Mar 18;2022:9014501.
doi: 10.1155/2022/9014501.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Goat anti Rabbit IgG (Fc):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
STAR121F
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Search for Batch Specific Datasheets

Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.

Goat anti rabbit IgG antibody has been used successfully as a secondary detection reagent for rabbit primary antibodies using immunohistochemical techniques (Redondo et al. 2013).

Target Species
Rabbit
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/200 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG antibody

  1. Kalluri, S.R. et al. (2010) Quantification and functional characterization of antibodies to native aquaporin 4 in neuromyelitis optica.
    Arch Neurol. 67:1201-8.
  2. Redondo, E. et al. (2014) Induction of interleukin-8 and interleukin-12 in neonatal ovine lung following experimental inoculation of bovine respiratory syncytial virus.
    J Comp Pathol. 150 (4): 434-48.
  3. Tyrsina, E. et al. (2019) Detection and quantification of VEGFR-1 in the nuclei of tumor cells by a new flow cytometry-based method.
    J Immunotoxicol. 16 (1): 74-81.
  4. Lin, W. et al. (2020) Rapid identification of anti-idiotypic mAbs with high affinity and diverse epitopes by rabbit single B-cell sorting-culture and cloning technology.
    PLoS One. 15 (12): e0244158.
  5. Li, Y. et al. (2022) Low-Temperature Plasma-Activated Medium Inhibited Proliferation and Progression of Lung Cancer by Targeting the PI3K/Akt and MAPK Pathways.
    Oxid Med Cell Longev. 2022: 9014501.

RRID
AB_567028
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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