PureBlu™ Nuclear Staining Dyes are designed to specifically stain the nuclei of cells in fixed and unfixed samples for flow cytometry, fluorescence microscopy and cell imaging applications.
Resuspend one tube of PureBlu Dye Powder in 500 µl of deionized H2O and dilute to 50 ml in PBS/media to prepare the working solution (PureBlu DAPI, 1 µg/ml (0.03 mM); PureBlu Hoechst 33342, 1.1 µg/ml (0.02 mM)). Note: the optimal concentration for different cell types and applications should be determined empirically.
|Optimal Excitation Laser, nm||S3e Cell Sorter||ZE5 Cell Analyzer||ZOE Fluorescent Cell Imager|
* Includes 5x50 µg vials of DAPI powder.
** Includes 5x56 µg vials of Hoechst 33342 powder.