VivaFix™ Cell Viability Assays

Vivafix Cell Viability Assays

Fixable viability dyes

VivaFix™ Cell Viability Assays allow accurate discrimination between live and dead cells in fixed or unfixed samples in Flow Cytometry, Cell Sorting and Microscopy.

In Flow Cytometry experiments the exclusion of dead cells in a sample is an important step to prevent errors in data interpretation due to non-specific fluorescence signals. In Microscopy cell viability and live:dead cell ratios are parameters that are frequently used to determine cell health.

VivaFix Cell Viability Dyes covalently bind to free primary amines which in live cells are present on the cell surface. Where the membrane is compromised, the dyes permeate the cells and react with intracellular primary amines. There is greater fluorescence due to increased content of accessible free amines in dead cells allowing them to be easily distinguished from live cells (Figure 1). In addition, they are available in a range of excitation and emission spectra for convenient addition to multi-color flow cytometry panels.

VivaFix Cell Viability Assay chemistry

Figure 1. VivaFix Cell Viability Assay chemistry. A, live cell with VivaFix Dye bound to surface primary amines. B, dead cell with VivaFix Dye bound to surface and intracellular primary amines.


VivaFix Fixable Viability Dyes Key Benefits

  • Fixed and unfixed cells can be stained
  • Easily distinguish between live and dead cell populations — at least 100-fold difference in fluorescence intensity
  • Available in a range of excitation and emission spectra to fit into multi-color panels

Description

Excitation Max, nm

Emission Max, nm

Optimal excitation laser

Catalog Number

VivaFix 353/442

353

442

355

1351111

VivaFix 410/450

410

450

405

1351112

VivaFix 408/512

408

512

405

1351113

VivaFix 398/550

398

550

405

1351114

VivaFix 498/521

498

521

488

1351115

VivaFix 547/573

547

573

561

1351116

VivaFix 583/603

583

603

561

1351117

VivaFix 649/660

649

660

640

1351118


Related products

We also offer non membrane permeable DNA-binding cell staining dyes excited by the 488 nm and 561 nm laser to allow you to assess the viability of your cells: