IgM Antibody | LO-MM-9

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  • Rat anti Mouse IgM Heavy Chain:Biotin
  • Rat anti Mouse IgM Heavy Chain:FITC
  • Rat anti Mouse IgM Heavy Chain
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LO-MM-9
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA199C, Edatasheet pdfdatasheet pdf0.25 mg
    MCA199
    MCA199BEdatasheet pdfdatasheet pdf0.5 mg
    MCA199B
    MCA199FFdatasheet pdfdatasheet pdf0.5 mg
    MCA199F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse IgM, clone LO-MM-9 is directed against the mu chain of mouse IgM.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      50% Glycerol
      0.1%Sodium Azide
      50%Glycerol
      0.1%Sodium Azide
    • Immunogen
      Polyclonal and monoclonal mouse IgM.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      positive regulation of endocytosis
      activation of MAPK activity
      transmembrane receptor activity
      B cell receptor complex
      B cell receptor signaling pathway
      positive regulation of peptidyl-tyrosine phosphorylation
      integral to membrane
      positive regulation of B cell proliferation
      humoral immune response mediated by circulating immunoglobulin
      antigen processing and presentation
      immunoglobulin complex, circulating
      perinuclear region of cytoplasm
      protein binding
      positive regulation of MAPKKK cascade
      early endosome to late endosome transport
      antigen binding
      external side of plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA250ng/ml
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry5ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml (as a coating antibody)
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This product may be used in an indirect ELISA or as a secondary reagent in a sandwich ELISA together with MCA1293 as the capture reagent and and Streptavidin:HRP (STAR5B) as the detection reagent.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgM Antibody Formats

    Formats Clone Applications Sizes available
    IgM Antibody : FITC LO-MM-9 F 0.5 mg
    IgM Antibody : Biotin LO-MM-9 E 0.5 mg
    IgM Antibody : Purified LO-MM-9 C, E 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
        MCA1212F

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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