IgG3 Antibody | LO-MG3-7

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  • Rat anti Mouse IgG3 Heavy Chain
  • Rat anti Mouse IgG3 Heavy Chain:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LO-MG3-7
  • Isotype
    IgM
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA423Edatasheet pdfdatasheet pdf0.25 mg
    MCA423
    MCA423BEdatasheet pdfdatasheet pdf0.5 mg
    MCA423B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse IgG3 Heavy Chain antibody, clone LO-MG3-7 recognizes the gamma 3 heavy chain of mouse IgG and does not cross react with other subclasses of murine IgG.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgM - liquid
      Purified IgM conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by affinity chromatography from tissue culture supernatant
      Purified IgM prepared by affinity chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.1%Sodium Azide
      0.09%Sodium Azide
      50%Glycerol
    • Immunogen
      Polyclonal and monoclonal mouse IgG3.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 1 mg/ml
      IgM concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      antigen binding
      integral to membrane
      plasma membrane
      extracellular region
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml as a coating antibody
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml1.0ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      This product may be used in an indirect ELISA or as a secondary reagent in a sandwich ELISA together with MCA1292 as the capture reagent and and Streptavidin:HRP (STAR5B) as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG3 Antibody Formats

    Formats Clone Applications Sizes available
    IgG3 Antibody : Purified LO-MG3-7 E 0.25 mg
    IgG3 Antibody : Biotin LO-MG3-7 E 0.5 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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