IFN Gamma Antibody | XMG1.2

//www.bio-rad-antibodies.com/mouse-ifn-gamma-antibody-xmg1-2-mca1548.html?RenderPageSpecific=1
IFN Gamma Antibody | XMG1.2 gallery image 1

Detection of Recombinant Mouse Interferon Gamma in Sandwich ELISA using Rat anti Mouse Interferon Gamma: Endotoxin Low (MCA1548EL) as a coating antibody and a biotinylated Rabbit anti Mouse Interferon Gamma as the detection reagent

Enlarge
  • IFN Gamma Antibody | XMG1.2 thumbnail image 1
  • Rat anti Mouse Interferon Gamma:RPE
  • Rat anti Mouse Interferon Gamma:FITC
  • Rat anti Mouse Interferon Gamma:Low Endotoxin
  • Rat anti Mouse Interferon Gamma
  • Rat anti Mouse Interferon Gamma:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    XMG1.2
  • Isotype
    IgG1
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1548FF *datasheet pdfdatasheet pdf0.1 mg
    MCA1548F
    MCA1548BEdatasheet pdfdatasheet pdf0.2 mg
    MCA1548B
    MCA1548E, F *datasheet pdfdatasheet pdf0.25 mg
    MCA1548
    MCA1548ELE, F *, FNdatasheet pdfdatasheet pdf0.5 mg
    MCA1548EL
    MCA1548PEF *datasheet pdfdatasheet pdf50 µg
    MCA1548PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse Interferon gamma antibody, clone XMG1.2 recognizes murine IFN gamma. Rat anti Mouse Interferon gamma antibody, clone XMG1.2 may be used for the detection of intracellular IFN gamma using flow cytometric techniques and has been shown to block the functional activity of murine interferon gamma.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.1% Gelatin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Recombinant mouse IFN gamma.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      defense response to bacterium
      positive regulation of interleukin-1 beta secretion
      negative regulation of myelination
      inflammatory cell apoptosis
      endoplasmic reticulum unfolded protein response
      antigen processing and presentation
      neutrophil apoptosis
      neutrophil chemotaxis
      positive regulation of interleukin-6 biosynthetic process
      response to virus
      positive regulation of MHC class II biosynthetic process
      positive regulation of isotype switching to IgG isotypes
      interferon-gamma receptor binding
      positive regulation of chemokine biosynthetic process
      positive regulation of interleukin-12 production
      defense response to protozoan
      positive regulation of nitric oxide biosynthetic process
      extracellular space
      negative regulation of growth of symbiont in host
      immune response
      positive regulation of transcription, DNA-dependent
      positive regulation of T cell proliferation
      intracellular
      positive regulation of interleukin-12 biosynthetic process
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)1/101/20
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry(1)Neat1/10
      (1)
      Membrane permeablisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Functional Assays
      Flow Cytometry(1)
      (1)
      Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1ug/ml10ug/ml
      Flow Cytometry(1)1/101/50
      (1)
      Membrane permeablisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.25ug/ml2.0ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IFN Gamma Antibody Formats

    Formats Clone Applications Sizes available
    IFN Gamma Antibody : Low Endotoxin XMG1.2 E, F *, FN 0.5 mg
    IFN Gamma Antibody : FITC XMG1.2 F * 0.1 mg
    IFN Gamma Antibody : Purified XMG1.2 E, F * 0.25 mg
    IFN Gamma Antibody : RPE XMG1.2 F * 50 µg
    IFN Gamma Antibody : Biotin XMG1.2 E 0.2 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG1 Negative Control:FITCMCA1211F100 TestsF
        MCA1211F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG1 Negative Control:Low EndotoxinMCA1211EL0.5 mgC, E, F
        MCA1211EL
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG1 Negative ControlMCA12111 mlC, E, F
        MCA1211

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...