CD8 Alpha Antibody | 53-6.7

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CD8 Alpha Antibody | 53-6.7 gallery image 1

Figure A. Alexa® 647 conjugated mouse anti human CD3 (MCA500A647) and RPE conjugated Rat IgG2a isotype control (MCA1212PE). Figure B. Alexa® 647 conjugated mouse anti human CD3 (MCA500A647) and RPE conjugated mouse anti human CD8 alpha (MCA2694PE). All experiments performed on red cell lysed murine splenocytes gated on lymphocytes in the presence of murine SeroBlock (BUF041A )

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CD8 Alpha Antibody | 53-6.7 gallery image 2

Figure A. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated Rat IgG2a isotype control (MCA1212F). Figure B. APC conjugated rat anti mouse CD3 (MCA500APC) and FITC conjugated rat anti mouse CD8 alpha (MCA2694F). All experiments performed on murine splenocytes in the presence of murine SeroBlock (BUF041A)

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CD8 Alpha Antibody | 53-6.7 gallery image 3

Figure A. RPE conjugated Rat anti Mouse Ly-6C (MCA2389PE) and FITC conjugated Rat IgG2a isotype control (MCA1212F). Figure B. RPE conjugated Rat anti Mouse Ly-6C (MCA2389PE) and FITC conjugated Rat anti Mouse CD8 alpha (MCA2694F). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

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CD8 Alpha Antibody | 53-6.7 gallery image 4

Published customer image:
Rat anti Mouse CD8α antibody, clone 53-6.7 (MCA2694) used to label CD8 expressing T cells in mouse brain by immunofluorescence.
Image caption:
Adoptive transfer of CD8+ cells successfully migrate to brain post induction of PIFS.
(A) Schematic illustrating the adoptive transfer of sorted perforin competent CD8+ cells into perforin-/- mice followed by induction of PIFS. C57BL/6 perforin-/- mice were irradiated with 400 rads of irradiation and then intravenously injected with 107 GFP+ CD8+ splenocytes (n = 10) or 107 Ly5.1+ CD8+ splenocytes (n = 10). Mice were intracranially infected with TMEV on the following day. PIFS-inducing VP2121–130 peptide or mock E7 control peptide were intravenously administered on day 7, during the peak of CD8 T cell expansion. MRI analysis was performed on the following day to visualize the extent of CNS vascular permeability and then the CNS was harvested for additional assays. Both negative (Experiment I) and positive (Experiment II) sort experiments yielded high purity of CD8+ T cell transfer. (B) Representative confocal microscopic images illustrating co-localization of Ly5.1 and CD8 protein (Experiment I). CNS-infiltrating Ly5.1+ cells colocalized with CD8+ cells 85.7% of the time as measured by confocal analysis. (C) Confocal microscopy showing of representative brain tissue slice showing successful transfer of GFP+ CD8+ T cells (Experiment II). Purity of transfer was analyzed via flow cytometry, 98.0% ± 0.5% of cells that were CD8 positive and GFP positive.

From: Johnson HL, Willenbring RC, Jin F, Manhart WA, LaFrance SJ, Pirko I, et al. (2014)
Perforin Competent CD8 T Cells Are Sufficient to Cause Immune-Mediated Blood-Brain Barrier Disruption.
PLoS ONE 9(10): e111401.

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CD8 Alpha Antibody | 53-6.7 gallery image 5

Published customer image:
Rat anti Mouse CD8α antibody, clone 53-6.7 used to stain CD8 expressing tissue in mouse pancreatic sections by immunofluorescence.
Image caption:
Determination of Kb/B22–29-tetramer+ CD8 T-cells in diabetic RIP-B7.1 tg mice.
(C) The numbers of Kb/B22–29-tetramer+ CD8 T-cells were determined during the course of pCI/ppinsΔA12–21-mediated EAD: group 1, health mice (n = 3) with blood glucose levels <200 mg/dl; group 2, early diabetic mice (n = 3) with blood glucose levels between 250-350 mg/dl; group 3, diabetic mice (n = 3) with severe diabetes (i.e., blood glucose levels between 400-550 mg/dl). Pancreata of representative mice out of groups 1 to 3 were analyzed histologically for CD8 T-cell influx (CD8+) or stained with hematoxylin-eosin (H&E).

From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013)
A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner.
PLoS ONE 8(8): e71746.

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CD8 Alpha Antibody | 53-6.7 gallery image 6

Published customer image:
Rat anti mouse CD8α antibody, clone 53-6.7 (MCA2694) used for the identification of CD8 expressing cells in the pancreata of normal and early diabetic mice by immunofluorescence
Image caption:
Ppins/(Kb/A12–21)-mediated recruitment of autoreactive T-cells into the pancreatic target tissue. PD-L1-/- mice were immunized with pCI/ppinsΔA12–21 (A) or with both, pCI/ppins+pCI/ppinsΔA12–21 vectors into the right and the left tibialis anterior muscles, respectively (B). Pancreata of representative healthy (A) and early diabetic mice (B) were analyzed histologically for insulin expression (insulin) and influx of CD8+ T-cells (CD8+), or stained with hematoxylin-eosin (H&E).

From: Schuster C, Brosi H, Stifter K, Boehm BO, Schirmbeck R (2013)
A Missing PD-L1/PD-1 Coinhibition Regulates Diabetes Induction by Preproinsulin-Specific CD8 T-Cells in an Epitope-Specific Manner.
PLoS ONE 8(8): e71746.

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  • Rat anti Mouse CD8 Alpha:RPE
  • Rat anti Mouse CD8 Alpha:FITC
  • Rat anti Mouse CD8 Alpha
  • Rat anti Mouse CD8 Alpha
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    53-6.7
  • Isotype
    IgG2a
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2694FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2694F
    MCA2694C, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2694
    MCA2694PEFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2694PE
    MCA2694TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2694T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD8 alpha antibody, clone 53-6.7 detects mouse CD8a, also known as Lyt-2. CD8α is the alpha chain of the CD8 antigen. CD8 is expressed on T-cells, and exists either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain.

      CD8 functions as a co-receptor with the T-cell receptor and mediates efficient cell to cell interactions within the immune system. The CD8 alpha chain specifically binds to class I MHC molecules.

      Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. Rat anti Mouse CD8 alpha antibody, clone 53-6.7 has also been used for depletion of CD8a+ cells.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      1%Bovine Serum Albumin
      0.09%Sodium Azide (NaN3)
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Mouse thymus or spleen.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      cell surface receptor linked signaling pathway
      external side of plasma membrane
      integral to membrane
      protein homodimerization activity
      positive regulation of calcium-mediated signaling
      T cell mediated immunity
      cytotoxic T cell differentiation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/20
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/20
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
      MCA2694 has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
    • Immunohistology
      MCA2694T has been reported as working well on Zinc-fixed paraffin-embedded sections. However, inconsistent results have been reported on formalin-fixed paraffin-embedded tissue sections.
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD8 Alpha Antibody Formats

    Formats Clone Applications Sizes available
    CD8 Alpha Antibody : FITC 53-6.7 F 0.1 mg
    CD8 Alpha Antibody : Purified 53-6.7 C, F, IP 25 µg | 0.25 mg
    CD8 Alpha Antibody : RPE 53-6.7 F 100 Tests/1ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
        MCA1212PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
        MCA1212F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

              Further Reading

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