IgG2a antibody
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Goat anti Rat IgG2a:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG2a
Antibody Quality and Performance Guarantee
| Goat anti Rat IgG2a antibody recognizes rat IgG2a as assessed by immunoelectrophoresis and ELISA. Goat anti Rat IgG2a antibody may cross react with IgG2a from other species. |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to rat IgG2a were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1 x 106 cells in 100ul.
References for IgG2a antibody
-
Platt, R. et al. (2011) Comparison of humoral and cellular immune responses to inactivated swine influenza virus vaccine in weaned pigs.
Vet Immunol Immunopathol. 142: 252-7.
- RRID
- AB_324440
- UniProt
- P20760
- Entrez Gene
- LOC679045
- GO Terms
- GO:0003823 antigen binding
View more products with IgG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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