CD41 Antibody | MWReg30

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CD41 Antibody | MWReg30 gallery image 1

Staining of mouse peripheral blood with Rat anti Mouse CD41 (MCA2245GA). Platelets stain strongly for CD41

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CD41 Antibody | MWReg30 gallery image 2

Published customer image:
Rat anti Mouse CD41 antibody, clone MWReg30 used for the evaluation of CD41 expressing megakaryocyte precursor numbers in murine haematopoietic organs by flow cytometry.
Image caption:
SHIP deficient mice have increased number of functional MKP in hematopoietic organs.
(A) BM, spleen, and PB MKP phenotype in SHIP-/- (-/-) and SHIPΔIP/ΔIP (ΔIP/ΔIP) mice. Shown are representative flow cytometry dot plots of CD41 (x-axis) versus c-Kit (y-axis) after gating on live and Lin- cells. MKP (Lin-c-Kit+CD41+) are represented in the right upper quadrants and MK (Lin-c-Kit-CD41+) are represented in the right lower quadrants. Percentages for each population are indicated on each plot. (B) Represented are the mean±standard error mean (SEM) (n?=?4 for experimental groups WT C57Bl6 and SHIP-/-, while n?=?6 for experimental groups WT 129SvJ and SHIPΔIP/ΔIP) of the absolute numbers of MKP in BM, spleen and PB of SHIP deficient mice (black columns) compared to respective WT littermates (gray columns). Mice strains specified in the graphs. (C) Represented is the mean±SEM (n?=?4 for experimental groups WT C57Bl6 and SHIP-/-, while n?=?6 for experimental groups WT 129SvJ and SHIPΔIP/ΔIP) of total body MKP numbers determined as the calculated sum of MKP in one whole spleen plus MKP in one femur ×16.6 (since one femur is estimated to contained 6% the total marrow) [3]. (D) Represented is the mean±SEM of CFU-MK colonies in BM and spleen of SHIP-/- (n?=?7) and WT (n?=?7) littermates. Statistical analysis was established using the Mann-Whitney test. p values indicated on each graph.

From: Perez LE, Desponts C, Parquet N, Kerr WG (2008)
SH2-Inositol Phosphatase 1 Negatively Influences Early Megakaryocyte Progenitors.
PLoS ONE 3(10): e3565.

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CD41 Antibody | MWReg30 gallery image 3

Published customer image:
Rat anti Mouse CD41 antibody, clone MWReg30 used for the demonstration of CD41 expressing megakaryocytes in murine spleen and bone marrow by immunohistochemistry on formalin fixed, paraffin embedded tissue sectons.
Image caption:
MK morphology and Glycoprotein IIB-IIIa (CD41) immunostain in BM and spleen of SHIP deficient mice.
(A). Hematoxylin-Eosin staining of BM and spleen of SHIP-/- (-/-) and wild type (WT) littermate controls. Spleen of SHIP deficient mice is characterized by a massive infiltrate with morphologically appearing MK (left panel) compared to WT littermates (right panel). BM of SHIP-/- mice contained hypolobulated micro-MK compared to hyperlobulated MK in WT BM. Magnification: ×60. Results are representative of those obtained in 3 animals per experimental group (B). Glycoprotein IIB-IIIA (CD41) immuno-histochemistry. Spleen of SHIP-/- mice contained a higher percentage of CD41+ cells compared to WT spleen. Magnification: ×40. BM of SHIP deficient mice contained comparable number of CD41+ cells compared to WT. Magnification: ×60.

From: Perez LE, Desponts C, Parquet N, Kerr WG (2008)
SH2-Inositol Phosphatase 1 Negatively Influences Early Megakaryocyte Progenitors.
PLoS ONE 3(10): e3565.

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CD41 Antibody | MWReg30 gallery image 4

Published customer image:
FITC labeled Rat anti Mouse CD41 antibody, clone MWReg30 used for the evaluation of CD41 expression on platelet,leucocyte aggregates by flow cytometry.
Image caption:
Effect of mTPOR-MBP on platelet-leukocyte adhesion in LPS model and in the cecal ligation and puncture (CLP) model. (A) Representative flow cytometry analysis of platelet-monocyte (upper lane) and platelet-neutrophil aggregates (lower lane) detected in mice injected with saline (Control) and in mice injected with LPS treated or not with mTPOR-MBP. The two left panels show an example of monocyte (upper panel) and neutrophil (lower panel) subpopulations, identified by size and granularity in the forward scatter (FS LIN) versus side scatter (SS LIN) dot plot, using a logic gate, over the total CD45-positive leukocyte population. The others panels are representative of CD45 versus CD41 dot plots obtained in the different experimental conditions. Platelet-monocyte and platelet-neutrophil aggregates were identified, within the logic gates previously selected, as CD45 and CD41 double-positive cells. (B-C) Quantification of the percentage of platelet-monocyte (panel B) and platelet-neutrophil (panel C) aggregates detected ex vivo by flow cytometry at the indicated time-points after LPS administration, in mice treated (dashed columns) or not (black columns) with mTPOR-MBP. The number of animals studied per experimental group was as follows: Control (C) = 10; mTPOR-MBP alone = 9; LPS 3h = 8; LPS 3h + mTPOR-MBP = 6; LPS 6h = 5; LPS 6h + mTPOR-MBP = 5. (D-E) Quantification of the percentage of platelet-monocyte (panel D) and platelet-neutrophil (panel E) aggregates detected ex vivo by flow cytometry in sham-operated mice (white columns) and septic mice 18 hours after CLP, treated (dashed columns) or not (black columns) with mTPOR-MBP. The number of animals studied per experimental group was as follows: Sham-operated mice = 5; CLP = 5; CLP + mTPOR-MBP = 5. * P < 0.05; ** P < 0.01; *** P <0.001.

From: Cuccurullo A, Greco E, Lupia E, De Giuli P, Bosco O, Martin-Conte E, et al. (2016)
Blockade of Thrombopoietin Reduces Organ Damage in Experimental Endotoxemia and Polymicrobial Sepsis.
PLoS ONE 11(3): e0151088.

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  • CD41 Antibody | MWReg30 thumbnail image 3
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  • Rat anti Mouse CD41:Biotin
  • Rat anti Mouse CD41
  • Rat anti Mouse CD41
  • Rat anti Mouse CD41:FITC
  • Rat anti Mouse CD41
  • Rat anti Mouse CD41:RPE
  • Rat anti Mouse CD41:FITC
  • Rat anti Mouse CD41:RPE
  • Rat anti Mouse CD41:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    MWReg30
  • Isotype
    IgG1
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2245FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2245F
    MCA2245GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2245GA
    MCA2245BFdatasheet pdfdatasheet pdf0.1 mg
    MCA2245B
    MCA2245C, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2245
    MCA2245PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2245PE
    MCA2245BTFdatasheet pdfdatasheet pdf25 µg
    MCA2245BT
    MCA2245TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2245T
    MCA2245FTFdatasheet pdfdatasheet pdf25 µg
    MCA2245FT
    MCA2245PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2245PET
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD41 antibody, clone MWReg30 recognizes the mouse integrin alpha IIb subunit CD41. CD41 is a ~125kDa single pass type 1 transmembrane glycoprotein expressed by platelets, megakaryocytes (Zhang et al. 2007), mast cells (Berlanga et al. 2005)and hematopoietic progenitors (Mitjavila-Garcia et al. 2002). CD41 forms a heterodimer with CD61. The CD41/CD61 complex is important for platelet adhesion and aggregation (Patel et al. 2003) acting as a receptor for many extracellular matrix proteins including fibronectin, thrombospondin and vitronectin (Weisel et al. 1992).

      Rat anti mouse CD41, clone MWReg30 has been reported to inhibit PMA induced aggregation in vitro and to induce hypothermia in vivo (Nieswandt et al. 1999).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 25 TestsReconstitute in 0.25 ml disilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Purified murine platelets.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      cell-matrix adhesion
      focal adhesion
      integrin-mediated signaling pathway
      external side of plasma membrane
      integrin complex
      receptor activity
      extracellular matrix binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD41 Antibody Formats

    Formats Clone Applications Sizes available
    CD41 Antibody : Purified MWReg30 C, F, IP 0.1 mg | 25 µg | 0.25 mg
    CD41 Antibody : FITC MWReg30 F 0.1 mg | 25 µg
    CD41 Antibody : RPE MWReg30 F 100 Tests | 25 Tests
    CD41 Antibody : Biotin MWReg30 F 0.1 mg | 25 µg
    • Copyright © 2016 Bio-Rad

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        Useful Reagents

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              Source Reference

              References

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