CD4 Antibody | YTS191.1

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CD4 Antibody | YTS191.1 gallery image 1

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor® 405 (MCA1767A405)

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CD4 Antibody | YTS191.1 gallery image 2

Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MCA1767FA)

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CD4 Antibody | YTS191.1 gallery image 3

Staining of mouse spleen cells with Rat anti Mouse CD4: APC (MCA1767APC)

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CD4 Antibody | YTS191.1 gallery image 4

Staining of mouse spleen cells with Rat anti Mouse CD4 (MCA1767GA)

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CD4 Antibody | YTS191.1 gallery image 5

Staining of mouse spleen cells with Rat anti Mouse CD4:RPE (MCA1767PE)

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CD4 Antibody | YTS191.1 gallery image 6

Staining of mouse spleen with Rat anti Mouse CD4 (MCA1767)

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CD4 Antibody | YTS191.1 gallery image 7

Published customer image:
Rat anti Mouse CD4 antibody, clone YTS191.1 used for the detection of CD4 positive lymphocytes in mouse liver metastases by immunohustochemistry and immunofluorescence on acetone trated cryosections.
Image caption:
(a) Immunohistochemistry and (b) immunofluorescence double staining: Representative image of CD4 (red)/CD8 (blue) (top) expression and Foxp3 (FITC)/CD4 (Cy3) (bottom) expression in liver metastases on day 10 (n = 7) compared to increased CD4 (red)/CD8 (blue) expression and Foxp3 (FITC)/CD4 (Cy3) expression on day 20. Immunohistochemistry: Nova red brick red color, Vector Blue blue color. Haemalaun blue color-nuclear counterstaining. Asterisk with indication lines show metastases next to normal tissue (×100 and ×400). Immunofluorescence: FITC green fluorescein isothiocyanate, Cy3 red and DAPI 4',6-diamidino-2-phenylindole blue - nuclear counterstaining (×250).

From: Grimm M, Gasser M, Bueter M, Strehl J, Wang J, Nichiporuk E, Meyer D, Germer CT, Waaga-Gasser AM, Thalheimer A. Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases. BMC Cancer. 2010 Mar 7;10:82.

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CD4 Antibody | YTS191.1 gallery image 8

Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin (MCA1767EL)

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CD4 Antibody | YTS191.1 gallery image 9

Published customer image:
Rat anti Mouse CD4 antibody, clone YTS191.1 used for the detection of CD4 positive cells in a mouse model of glomerulonephritis by immunohistochemistry on formalin fixed, paraffin embedded renal tissue sections.
Image caption:
Eplerenone inhibits the infiltration of inflammatory cells. (A) Representative pictures of CD4 (a, b), CD8 (c, d) and F4/80 (e, f) immunohistochemistry 14 days after induction of nephritis are shown (magnification ×400). In control mice an accentuated interstitial and periglomerular infiltration (arrows) of CD4+ (a) and CD8+ (c) T cells as well as of macrophages (e) was found, which was reduced by Epl treatment ((b) for CD4+, (d) for CD8+ and (f) for macrophages). (B) The infiltration of CD4+ T cells, counted in six adjacent high-power fields in cortex and medulla, was significantly diminished in Epl-treated mice () at day 14 (*p<0.05), CD8+ T cell infiltration was significantly reduced on day 14 (*p<0.05) and showed a strong trend towards reduction on day 7 (p=0.087) compared to control mice (?). (C) Macrophage infiltration was significantly reduced in Epl-treated mice () on days 7 and 14 (*p<0.05) in comparison to controls (?).

From: Zitt E, Eller K, Huber JM, Kirsch AH, Tagwerker A, Mayer G, Rosenkranz AR. The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis. Int J Clin Exp Pathol. 2011 Aug 15;4(6):606-15.

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  • Rat anti Mouse CD4
  • Rat anti Mouse CD4:Biotin
  • Rat anti Mouse CD4:Alexa Fluor® 700
  • Rat anti Mouse CD4
  • Rat anti Mouse CD4
  • Rat anti Mouse CD4:RPE-Alexa Fluor® 647
  • Rat anti Mouse CD4:Low Endotoxin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    YTS191.1
  • Isotype
    IgG2b
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1767BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1767B
    MCA1767GAC *, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1767GA
    MCA1767C *, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA1767
    MCA1767ELC *, F, FNdatasheet pdfdatasheet pdf0.5 mg
    MCA1767EL
    MCA1767A700Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1767A700
    MCA1767P647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA1767P647
    MCA1767TC *, Fdatasheet pdfdatasheet pdf25 µg
    MCA1767T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD4 antibody, clone YTS191.1 recognizes the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes.

      Rat anti Mouse CD4 antibody, clone YTS191.1 exhibits depleting activity when used in vivo (Bemelman et al. 1998).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 700 - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Alexa Fluor® 647 - lyophilised
      Purified IgG - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      None present
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
      Pack Size: 0.1 mgIgG concentration 1 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
    • GO Terms
      membrane raft
      cell adhesion
      cytokine production
      positive regulation of T cell activation
      positive regulation of peptidyl-tyrosine phosphorylation
      endoplasmic reticulum membrane
      external side of plasma membrane
      immune response
      endoplasmic reticulum lumen
      cell surface receptor linked signaling pathway
      T cell selection
      positive regulation of calcium-mediated signaling
      integral to membrane
      response to protein stimulus
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.25 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.25 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/50
      1/100Pack Size: 0.25 mg, 25 µg
      1/200Pack Size: 0.1 mg
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/1001/200
      Functional Assays
      Immunohistology - Paraffin
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD4 Antibody Formats

    Formats Clone Applications Sizes available
    CD4 Antibody : Alexa Fluor® 700 YTS191.1 F 100 Tests/1ml
    CD4 Antibody : Biotin YTS191.1 F 0.1 mg
    CD4 Antibody : Low Endotoxin YTS191.1 C *, F, FN 0.5 mg
    CD4 Antibody : Purified YTS191.1 C *, F 0.1 mg | 25 µg | 0.25 mg
    CD4 Antibody : RPE-Alexa Fluor® 647 YTS191.1 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad

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          Recommended Positive Controls

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              References

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