CD4 antibody | YTS177.9
Rat anti Mouse CD4:Low Endotoxin
- Product Type
- Monoclonal Antibody
- Clone
- YTS177.9
- Isotype
- IgG2a
- Specificity
- CD4
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with the Mouse CD4 antigen, non polymorphic epitope (Qin et al. 1990). Rat anti Mouse CD4 antibody, Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with CD4 transfectants (Cobbold et al. 1990).Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Mouse spleen cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20°C only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/200 |
| Functional Assays | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control:Low Endotoxin | MCA1212EL | F | 0.5 mg |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control:Low Endotoxin | ||||||
References for CD4 antibody
-
Qin, S.X. et al. (1990) Induction of tolerance in peripheral T cells with monoclonal antibodies.
Eur J Immunol. 20 (12): 2737-45. -
Cobbold, S.P. et al. (1990) The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Eur J Immunol. 20 (12): 2747-55. -
Wise, M.P. et al. (1998) Linked suppression of skin graft rejection can operate through indirect recognition.
J Immunol. 161 (11): 5813-6. -
Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
J Immunol. 179: 3648-54. -
Saunders, A. et al. (2009) Expression of GIMAP1, a GTPase of the immunity-associated protein family, is not up-regulated in malaria.
Malar J. 8: 53. -
Agua-Doce, A. and Graca, L. (2011) Prevention of house dust mite induced allergic airways disease in mice through immune tolerance.
PLoS One. 6: e22320. -
Carlring, J. et al. (2012) Conjugation of lymphoma idiotype to CD40 antibody enhances lymphoma vaccine immunogenicity and antitumor effects in mice.
Blood. 119 (9): 2056-65. -
Kim, I. et al. (2017) Ibrutinib suppresses alloantibody responses in a mouse model of allosensitization.
Transpl Immunol. 45: 59-64.
View The Latest Product References
-
Szuster-Ciesielska, A. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)(2) of Plasmodium falciparum in a Mouse Model.
J Immunol Res. 2019: 9264217.
- Synonyms
- L3T4 Antigen
- LY-4
- RRID
- AB_808423
- UniProt
- P06332
- Entrez Gene
- Cd4
- GO Terms
- GO:0001816 cytokine production
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005788 endoplasmic reticulum lumen
- GO:0005789 endoplasmic reticulum membrane
- GO:0006955 immune response
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0009897 external side of plasma membrane
- GO:0045058 T cell selection
- View More GO Terms
- GO:0045121 membrane raft
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050850 positive regulation of calcium-mediated signaling
- GO:0050870 positive regulation of T cell activation
- GO:0051789 response to protein stimulus
MCA1107EL
158335
158727
159642
1712
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