CD206 Antibody | MR5D3

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CD206 Antibody | MR5D3 gallery image 1

Staining of J774 cell line with Rat anti Mouse CD206 following permeabilization with Leucoperm (BUF09)

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CD206 Antibody | MR5D3 gallery image 2

Staining of mouse peritoneal macrophages with Rat anti Mouse CD206:Alexa Fluor® 488 (MCA2235A488)

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CD206 Antibody | MR5D3 gallery image 3

Staining of mouse peritoneal macrophages with Rat anti Mouse CD206:Alexa Fluor® 647 (MCA2235A647)

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CD206 Antibody | MR5D3 gallery image 4

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MCA2235) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Low power

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CD206 Antibody | MR5D3 gallery image 5

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MCA2235) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Medium power

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CD206 Antibody | MR5D3 gallery image 6

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MCA2235) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power

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CD206 Antibody | MR5D3 gallery image 7

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MCA2235), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power

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CD206 Antibody | MR5D3 gallery image 8

Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 (MCA2235), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 (MCA1108), red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power

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CD206 Antibody | MR5D3 gallery image 9

Published customer image:
Rat anti Mouse CD206 antibody, clone MR5D3 (MCA1957) used for the evaluation of mannose expression by primary microglia from wild type and IL-1 knockout mice by western blotting of isolated microglial lysates.
Image caption:
Western blot analysis to identify the type of activation of adult primary microglial cells from wild-type and IL-1 KO mice stimulated by vehicle, IFNγ or IL-4 with or without IL-1β. (A) Representative western blotting data of primary MG produced from wild-type (wild) and IL-1 KO mice exposed for 24 hours to vehicle, IFNγ or IL-4 with or without IL-1β. Each lane expected to CD206 blotting applied 8 μg of reduced samples. Non-reduced samples (5 μg) were applied to detect CD206. Densitometric analysis of STAT1 (B), COX2 (C), iNOS (D), Ym1 (E), Arg-1 (F) and CD206 (G) (n = 3 each group). (B) STAT1 level is increased by exposure of cells to IFNγ, but not to IL-4. The level is not influenced by co-treatment of cells with IL-1β. (C) The COX2 level is increased by exposure of cells to IL-1β but not to IFNγ or IL-4 alone. Co-treatment with IL-1β and IL-4 tends to increase the COX2 level synergistically. (D) While the iNOS level is not increased significantly by exposure of cells to IFNγ or IL-4, the level increased synergistically in response to co-treatment with IFNγ and IL-1β. (D) The level of Ym1 is increased by exposure of cells to IL-4 and further increased by co-treatment with IL-1β. (E) The Arg-1 level is also increased by exposure of cells to IL-4 and further increased by co-treatment with IL-1β. (G) The CD206 level is increased slightly by exposure of cells to IL-4, but not with co-treatment with IL-4 and IL-1β. Data are expressed as mean ± SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared to the vehicle-treated group without IL-1ß for each genotype (One-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX2, cyclooxygenase 2; iNOS, inducible NO synthase.

From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury.
J Neuroinflammation. 2012 Apr 7;9:65.

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CD206 Antibody | MR5D3 gallery image 10

Published customer image:
Rat anti Mouse CD206 antibody, clone MR5D3 (MCA1957) used for the evaluation of mannose expression by primary microglia from wild type and IL-1 knockout mice by western blotting of isolated microglial lysates.
Image caption:
Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1β. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1β. Each lane expected to CD206 blotting were applied 7 µg of reduced samples. Non-reduced samples (5 μg) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1β and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1β and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1β. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1β. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1β; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean ± SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1β for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.

From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury.
J Neuroinflammation. 2012 Apr 7;9:65.

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CD206 Antibody | MR5D3 gallery image 11

Published customer image:
Rat anti Mouse CD206 antibody, clone MR5D3 used for the detection of mannose receptor expressing macrophages in hind limbs of control and ischemia induced mice by immunofluorescence.
Image caption:
Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p<0.01, *** p<0.001.

From: van den Hengel LG, Hellingman AA, Nossent AY, van Oeveren-Rietdijk AM, de Vries MR, Spek CA, et al. (2013) Protease-Activated Receptor (PAR)2, but Not PAR1, Is Involved in Collateral Formation and Anti-Inflammatory Monocyte Polarization in a Mouse Hind Limb Ischemia Model.
PLoS ONE 8(4): e61923.

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CD206 Antibody | MR5D3 gallery image 12

Published customer image:
Rat anti Mouse CD206 antibody, clone MR5D3 used for the evaluation of alternative macrophage activation in the lungs of fibrotic IFNγ knockout and wild type mice by flow cytometry on isolated cells.
Image caption:
Elevated levels of alternative macrophage activation are observed in lung of fibrotic IFNγR-/- C57Bl/6 mice. 8–12 week old C57Bl/6 IFNγR-/- mice were intranasally infected with 1x105 pfu MHV68 (WT or M1st) and sacrificed at indicated times post infection (n = 3–4 mice/group at each timepoint). Whole lungs were harvested and assessed for macrophage population and phenotype. (A-B) Absolute number of alveolar and interstitial macrophages were quantified and assessed for alternative activation using RELMα and CD206 expression.

From: O'Flaherty BM, Matar CG, Wakeman BS, Garcia A, Wilke CA, Courtney CL, et al. (2015) CD8+ T Cell Response to Gammaherpesvirus Infection Mediates Inflammation and Fibrosis in Interferon Gamma Receptor-Deficient Mice.
PLoS ONE 10(8): e0135719.

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CD206 Antibody | MR5D3 gallery image 13

Published customer image:
Rat anti Mouse CD206 antiboddy, clone MR5D3 (MCA2235GA) used for the illustration of mannose receptor expressing cells in murine liver by immunofluorescence.
Image caption:
Immunohistochemical analysis of expression of FPR1 in human and mouse liver tissue.
Liver sections were fixed with cold acetone for 10 min, washed in PBS and incubated overnight at 4°C with polyclonal rabbit anti-FPR1 (1:200), followed by Alexa Fluor®-488 goat anti-rabbit (1:1000). Intense FPR1 staining can be observed along the sinusoids in mouse liver (A), while in human liver the staining was more evenly localized to both LSECs and Heps (B). Double staining was performed using polyclonal rabbit anti-FPR1, rat anti-mouse CD206, and goat anti-human CD206, followed by Alexa Fluor®-488 and Alexa Fluor®-594 secondary antibodies, respectively.

From: Citation: Øie CI, Snapkov I, Elvevold K, Sveinbjørnsson B, Smedsrød B (2016)
FITC Conjugation Markedly Enhances Hepatic Clearance of N-Formyl Peptides.
PLoS ONE 11(8): e0160602. doi:10.1371/journal.pone.0160602

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  • Rat anti Mouse CD206:FITC
  • Rat anti Mouse CD206:Alexa Fluor® 488
  • Rat anti Mouse CD206:Alexa Fluor® 647
  • Rat anti Mouse CD206:Biotin
  • Rat anti Mouse CD206:Biotin
  • Rat anti Mouse CD206
  • Rat anti Mouse CD206:Low Endotoxin
  • Rat anti Mouse CD206:FITC
  • Rat anti Mouse CD206:RPE
  • Rat anti Mouse CD206
  • Rat anti Mouse CD206:RPE
  • Rat anti Mouse CD206
  • Rat anti Mouse CD206:Alexa Fluor® 488
  • Rat anti Mouse CD206:FITC
  • Rat anti Mouse CD206:FITC
  • Rat anti Mouse CD206:Alexa Fluor® 647
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a 175kD type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells, and in vitro derived dendritic cells.
    • Product Type
      Monoclonal Antibody
    • Clone
      MR5D3
    • Isotype
      IgG2a
    7 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2235BF *datasheet pdfdatasheet pdf0.1 mg
      MCA2235B
      MCA2235FF *datasheet pdfdatasheet pdf0.1 mg
      MCA2235F
      MCA2235GAC, F *, IF, IPdatasheet pdfdatasheet pdf0.1 mg
      MCA2235GA
      MCA2235C, F *, IF, IPdatasheet pdfdatasheet pdf0.25 mg
      MCA2235
      MCA2235ELC, F *, IPdatasheet pdfdatasheet pdf0.5 mg
      MCA2235EL
      MCA2235FBF *datasheet pdfdatasheet pdf0.5 mg
      MCA2235FB
      MCA2235PEF *datasheet pdfdatasheet pdf100 Tests
      MCA2235PE
      MCA2235A647F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA2235A647
      MCA2235A488F *datasheet pdfdatasheet pdf100 Tests/1ml
      MCA2235A488
      MCA2235FTF *datasheet pdfdatasheet pdf25 µg
      MCA2235FT
      MCA2235BTF *datasheet pdfdatasheet pdf25 µg
      MCA2235BT
      MCA2235TC, F *, IF, IPdatasheet pdfdatasheet pdf25 µg
      MCA2235T
      MCA2235PETF *datasheet pdfdatasheet pdf25 Tests
      MCA2235PET
      MCA2235A488TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA2235A488T
      MCA2235A647TF *datasheet pdfdatasheet pdf25 Tests/0.25ml
      MCA2235A647T
      MCA2235FAF *datasheet pdfdatasheet pdf50 µg
      MCA2235FA
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002).

        The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005).

        Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
      • Intended Use
      • Target Species
        Mouse
      • Product Form
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG conjugated to Biotin - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid.
        Purified IgG - liquid
        Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
        Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid.
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor® 488 - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
        Purified IgG conjugated to Alexa Fluor® 647 - liquid
      • Reconstitution
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
        Pack Size: 100 TestsReconstitute with 1 ml distilled water
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        None Present
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09%Sodium Azide
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        5% Sucrose
        0.09%Sodium Azide
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
        0.09%Sodium Azide
        1%Bovine Serum Albumin
      • Immunogen
        Chimaeric CRD4-7-Fc protein
      • Purity
      • Approx. Protein Concentrations
        Pack Size: 0.1 mg, 25 µg, 50 µgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.05 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 0.1 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        Pack Size: 0.1 mg, 25 µg, 50 µgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 1.0 mg/ml
        IgG concentration 0.05 mg/ml
        Pack Size: 0.1 mg, 25 µg, 50 µgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        Pack Size: 0.1 mg, 25 µg, 50 µgIgG concentration 0.1 mg/ml
        Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
        IgG concentration 0.05 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line
      • Storage
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -20oC only.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC.

        DO NOT FREEZE

        This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.

        This product should be stored undiluted.

        Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

        Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        12 months from date of despatch.
        18 months from date of despatch.
        12 months from date of reconstitution.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        transmembrane receptor activity
        integral to membrane
        cell surface
        mannose binding
        endocytosis
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)neat
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 0.5 mg
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat1/10
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/101/20
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)neat
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 0.5 mg
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
        For better results an overnight incubation at 4°C is recommended

      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/101/20
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
        For better results an overnight incubation at 4°C is recommended

      • Application NameYesNoMin DilutionMax Dilution
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Flow Cytometry(1)1/101/20
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)neat
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 0.5 mg
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)neat
        NeatPack Size: 0.1 mg, 25 µg
        1/10Pack Size: 0.5 mg
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry(1)Neat
        (1)
        CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.

      • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 106 cell in 100ul.
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD206 Antibody Formats

      Formats Clone Applications Sizes available
      CD206 Antibody : FITC MR5D3 F * 0.1 mg | 50 µg | 25 µg | 0.5 mg
      CD206 Antibody : Biotin MR5D3 F * 0.1 mg | 25 µg
      CD206 Antibody : RPE MR5D3 F * 25 Tests | 100 Tests
      CD206 Antibody : Alexa Fluor® 488 MR5D3 F * 25 Tests/0.25ml | 100 Tests/1ml
      CD206 Antibody : Alexa Fluor® 647 MR5D3 F * 100 Tests/1ml | 25 Tests/0.25ml
      CD206 Antibody : Purified MR5D3 C, F *, IF, IP 25 µg | 0.25 mg | 0.1 mg
      CD206 Antibody : Low Endotoxin MR5D3 C, F *, IP 0.5 mg
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A
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        Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
        STAR131A
        Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
        STAR131B
        Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
        STAR71D549GA
        Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
        STAR71D649GA
        Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
        STAR16D800GA
        Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
        STAR71D800GA
        Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
        STAR69
        Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
        STAR17B
        Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
        STAR72
        Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
        STAR21B
        Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
        STAR73
        Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
        STAR20A

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
          MCA1212A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647100 Tests/1mlF
          MCA1212A647
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Low EndotoxinMCA1212EL0.5 mgF
          MCA1212EL
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
          MCA1212PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:RPEMCA1212PE100 TestsF
          MCA1212PE
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative ControlMCA12121 mlE, F
          MCA1212
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 488MCA1212A488100 Tests/1mlF
          MCA1212A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:FITCMCA1212F100 TestsF
          MCA1212F
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rat IgG2a Negative Control:Alexa Fluor® 647MCA1212A647100 Tests/1mlF
          MCA1212A647

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B

            Recommended Positive Controls

              Histology Controls

                References

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