CD200 Antibody | OX-90

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CD200 Antibody | OX-90 gallery image 1

Figure A. Alexa647® conjugated rat anti mouse CD22 (MCA2187A647) and RPE conjugated Rat IgG2b isotype control (MCA1212PE). Figure B. Alexa647® conjugated rat anti mouse CD22 (MCA500PE) and RPE conjugated rat anti mouse CD200 (MCA1958PE). All experiments performed on murine bone marrow in the presence of murine SeroBlock (BUF041A).

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  • CD200 Antibody | OX-90 thumbnail image 1
  • Rat anti Mouse CD200
  • Rat anti Mouse CD200:RPE
  • Rat anti Mouse CD200:Low Endotoxin
  • Rat anti Mouse CD200:FITC
  • Rat anti Mouse CD200
  • Rat anti Mouse CD200:FITC
  • Rat anti Mouse CD200:Biotin
  • Rat anti Mouse CD200:RPE
  • Rat anti Mouse CD200
  • Rat anti Mouse CD200:FITC
  • Rat anti Mouse CD200:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    OX-90
  • Isotype
    IgG2a
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1958GAC, E, Fdatasheet pdfdatasheet pdf0.1 mg
    MCA1958GA
    MCA1958FFdatasheet pdfdatasheet pdf0.1 mg
    MCA1958F
    MCA1958BFdatasheet pdfdatasheet pdf0.1 mg
    MCA1958B
    MCA1958C, E, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA1958
    MCA1958ELC, E, Fdatasheet pdfdatasheet pdf0.5 mg
    MCA1958EL
    MCA1958PEFdatasheet pdfdatasheet pdf100 Tests
    MCA1958PE
    MCA1958TC, E, Fdatasheet pdfdatasheet pdf25 µg
    MCA1958T
    MCA1958FTFdatasheet pdfdatasheet pdf25 µg
    MCA1958FT
    MCA1958BTFdatasheet pdfdatasheet pdf25 µg
    MCA1958BT
    MCA1958PETFdatasheet pdfdatasheet pdf25 Tests
    MCA1958PET
    MCA1958FAFdatasheet pdfdatasheet pdf50 µg
    MCA1958FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2.

      CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.

      Studies have suggested that the CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1ml distilled water
      Pack Size: 100 TestsReconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Mouse CD200-rat CD4 fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 1.0mg/ml
      IgG concentration 0.1mg/ml
      IgG concentration 0.1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised rats were fused with cells of the rat Y3 myeloma cell line
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 100 TestsPrior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 25 TestsStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4ºC or at -20ºC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Flow Cytometry
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
      Mouse spleen
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Mouse spleen
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Mouse spleen
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
      Mouse spleen
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD200 Antibody Formats

    Formats Clone Applications Sizes available
    CD200 Antibody : RPE OX-90 F 25 Tests | 100 Tests
    CD200 Antibody : FITC OX-90 F 0.1 mg | 25 µg | 50 µg
    CD200 Antibody : Low Endotoxin OX-90 C, E, F 0.5 mg
    CD200 Antibody : Purified OX-90 C, E, F 0.25 mg | 25 µg | 0.1 mg
    CD200 Antibody : Biotin OX-90 F 25 µg | 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      STAR131B
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      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
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      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
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      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
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      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
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      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
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      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
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      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
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      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
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      STAR71D649GA
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      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              Mouse spleen
              Mouse spleen
              Mouse spleen
              Mouse spleen
              • Application NameReference Images
                Flow Cytometry
                Immunohistology - Frozen

              References

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