CD13 Antibody | ER-BMDM1

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CD13 Antibody | ER-BMDM1 gallery image 1

Staining of J774 cells with Rat anti Mouse CD13:Alexa Fluor® 488 (MCA2395A488)

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CD13 Antibody | ER-BMDM1 gallery image 2

Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13 (MCA2395)

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CD13 Antibody | ER-BMDM1 gallery image 3

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MCA2395) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) Low power

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CD13 Antibody | ER-BMDM1 gallery image 4

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MCA2395) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) Medium power

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CD13 Antibody | ER-BMDM1 gallery image 5

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MCA2395) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) High power

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CD13 Antibody | ER-BMDM1 gallery image 6

Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MCA2395) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) Low power

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CD13 Antibody | ER-BMDM1 gallery image 7

Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MCA2395) followed by horseradish peroxidase conjugated Goat anti Rat IgG (STAR72) High power

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  • Rat anti Mouse CD13
  • Rat anti Mouse CD13
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    ER-BMDM1
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2395C, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA2395
    MCA2395TC, Fdatasheet pdfdatasheet pdf25 µg
    MCA2395T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse CD13 antibody, clone ER-BMDM1 recognizes the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.

      In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.

      Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Balb/c bone marrow derived macrophages.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      aminopeptidase activity
      metallopeptidase activity
      zinc ion binding
      cell differentiation
      integral to membrane
      angiogenesis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.

    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD13 Antibody Formats

    Formats Clone Applications Sizes available
    CD13 Antibody : Purified ER-BMDM1 C, F 25 µg | 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat IgG2a Negative ControlMCA12121 mlE, F
        MCA1212

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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