Panitumumab Antibody | AbD23897_hIgG1

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Human anti panitumumab specificity ELISA

A microtiter plate was coated over night with various antigens at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23897_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti panitumumab specificity titration ELISA

A microtiter plate was coated over night with panitumumab, human IgG2/kappa, adalimumab or human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using HRP conjugated Human anti panitumumab (clone AbD23897_hIgG1) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data for binding to panitumumab is shown as the mean of three measurements

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Panitumumab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti panitumumab (clone AbD23894, HCA264) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of panitumumab. Detection was performed using HRP conjugated Human anti panitumumab (clone AbD23897_hIgG1, HCA265P) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Human anti panitumumab bridging ELISA for Anti-Drug Antibody (ADA) assay development

A microtitre plate was coated over night with panitumumab at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of human anti panitumumab HCA265 (clone AbD23897_hIgG1) or HCA266 (clone AbD23895_hIgG1). Detection was performed using HRP conjugated panitumumab. Data is shown as the mean of three measurements

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Human anti panitumumab inhibits the binding of panitumumab to EGFR

A microtiter plate was coated over night with human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of panitumumab (0.3 μg/ml) plus increasing concentrations of Human anti panitumumab (clone AbD23897) in the monovalent Fab format was added. Free panitumumab, still capable of binding to the EGFR-coated plate, was detected using Goat anti Human IgG F(ab')2 HRP (0500-0099) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements

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  • Human anti Panitumumab
  • Human anti Panitumumab:HRP
(Rated 0.0 out of 5 based on 0 customer reviews)
    Anti-panitumumab antibody is a recombinant, neutralizing, anti-idiotypic antibody in fully human IgG1 format for bioanalytical assays to measure panitumumab and biosimilars. It can be used as detection antibody in a PK bridging ELISA with HCA264, and as reference standard in an ADA bridging ELISA.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD23897_hIgG1
    • Isotype
      IgG1
    2 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA265Edatasheet pdfdatasheet pdf0.1 mg
      HCA265
      HCA265PEdatasheet pdfdatasheet pdf0.1 mg
      HCA265P
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
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      • Human anti panitumumab, clone AbD23897_hIgG1, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the fully human monoclonal antibody drug panitumumab and inhibits it binding to its target, epidermal growth factor receptor (EGFR). The antibody can be used to measure the levels of panitumumab and biosimilar products in bioanalytical assays.

        Clone AbD23897_hIgG1 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a reference standard in an anti-drug antibody (ADA) assay. Additionally the antibody can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in full immunoglobulin format, is recommended as the detection antibody, paired with the antibody clone AbD23894 (HCA264) in monovalent Fab format, as the capture antibody.

        Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signaling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer.

        View a summary of all anti panitumumab antibodies
      • Intended Use
      • Product Form
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line.
        Human IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. Conjugated to horseradish peroxidase (HRP) - liquid.
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein A
        Purified IgG prepared by affinity chromatography on Protein A
      • Preservative Stabilisers
        0.01% Thiomersal
        0.01% Thiomersal
      • Immunogen
        Panitumumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD = 0.3 nM by real time, label free molecular interaction analysis on immobilized panitumumab.
      • Approx. Protein Concentrations
        IgG concentration 0.5 mg/ml
        IgG concentration 0.1 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at -70oC.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted.
      • Shelf Life
        12 months from date of despatch
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
        Vectibix® is a registered trademark of Amgen.
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
        Vectibix® is a registered trademark of Amgen.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA
      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • Recommended Protocol
      • ELISA
        This product may be used in direct or indirect ELISA and in an ELISA bridging assay as a detection antibody together, when conjugated to HRP, with HCA264 as the capture reagent. It is also suitable as a fully human control or calibrator antibody in an ADA bridging ELISA.
        Protocol: PK bridging ELISA to measure free drug
        Protocol: ADA bridging ELISA
      • ELISA
        This product may be used as a detection antibody together with HCA264 as the capture reagent.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use

      Additional Panitumumab Antibody Formats

      Formats Clone Applications Sizes available
      Panitumumab Antibody : HRP AbD23897_hIgG1 E 0.1 mg
      Panitumumab Antibody : Purified AbD23897_hIgG1 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            LYNX Rapid HRP Antibody Conjugation KitLNK002P3 Conjugations For 400µg AntibodyCJ
            LNK002P
            Human anti PanitumumabHCA2640.1 mgE
            HCA264
            Human anti PanitumumabHCA2660.1 mgE
            HCA266
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti PanitumumabHCA2640.1 mgE
            HCA264
            Human anti PanitumumabHCA2660.1 mgE
            HCA266
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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