Panitumumab Antibody | AbD23894

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Panitumumab Antibody | AbD23894 gallery image 1

Human anti panitumumab specificity titration ELISA

A microtiter plate was coated over night with panitumumab, human IgG2, adalimumab or human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti panitumumab (clone AbD23894) titrated to the given concentrations in PBST, followed by Mouse anti DYKDDDDK:HRP (MCA4764P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data for binding to panitumumab is shown as the mean of three measurements

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Human anti panitumumab specificity ELISA

A microtiter plate was coated over night with various antigens at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, detection was performed using Human anti panitumumab (clone AbD23894) at a concentration of 2 μg/ml, followed by Mouse anti DYKDDDDK:HRP (MCA4764P) in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate

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Panitumumab bridging ELISA for Pharmacokinetic (PK) assay development

A microtiter plate was coated over night with Human anti panitumumab (clone AbD23894) at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, 10% human serum was added spiked with increasing concentrations of panitumumab. Detection was performed using HRP-conjugated Human anti panitumumab (clone AbD23897_hIgG1, HCA265) at a concentration of 2 μg/ml in HISPEC assay diluent (BUF049A) and QuantaBlu™ fluorogenic peroxidase substrate. Data is shown as the mean of three measurements. HRP-conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit®

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Human anti panitumumab inhibits the binding of panitumumab to EGFR

A microtiter plate was coated over night with human EGFR at a concentration of 1 μg/ml. After washing and blocking with PBST+5% BSA, a pre-incubated mixture of panitumumab (0.3 μg/ml) plus increasing concentrations of Human anti panitumumab (clone AbD23894, HCA264) in the monovalent Fab format was added. Free panitumumab, still capable of binding to the human EGFR coated plate, was detected using a HRP-conjugated goat anti human Fab (0500-0099) antibody and QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements

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  • Human anti Panitumumab
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    Anti-panitumumab antibody is a recombinant, neutralizing, anti-idiotypic monovalent Fab antibody; it is ideal for bioanalytical assays to measure levels of panitumumab and biosimilars. It is recommended as capture antibody in a PK bridging ELISA format with HCA265 for detection.
    • Product Type
      Monoclonal Antibody
    • Clone
      AbD23894
    • Isotype
      HuCAL Fab monovalent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      HCA264Edatasheet pdfdatasheet pdf0.1 mg
      HCA264
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Human anti panitumumab clone AbD23894, is a paratope specific, high affinity, anti-idiotypic antibody that specifically recognizes the monoclonal antibody drug panitumumab and inhibits it binding to its target, epidermal growth factor receptor (EGFR). The antibody can be used to measure the levels of panitumumab and biosimilar products in bioanalytical assays.

        Our anti panitumumab antibodies can be used to develop a pharmacokinetic (PK) bridging assay to measure free drug. This antibody, in monovalent Fab format, is recommended as the capture antibody, paired with an antibody in full immunoglobulin (Ig) format as the detection antibody, clone AbD23897_hIgG1 (HCA265) or clone AbD23895_hIgG1 (HCA266). The antibody in Ig format can also be used to develop and calibrate immune response assays to measure the anti-drug antibody (ADA) response in patient sera.

        Panitumumab (Vectibix®) is a fully human monoclonal antibody (IgG2/kappa) specific to the transmembrane protein EGFR. The drug binds to the extracellular domain of the EGFR, inhibiting the activation of the intracellular signalling cascade. It is approved by the FDA and EMA for the treatment of EGFR-expressing metastatic colorectal cancer.

        View a summary of all anti panitumumab antibodies
      • Intended Use
      • Product Form
        A monovalent human recombinant Fab selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
      • Reconstitution
      • Preparation
        Metal chelate affinity chromatography
      • Preservative Stabilisers
        0.01% Thiomersal
      • Immunogen
        Panitumumab
      • Purity
      • Affinity
        The monovalent intrinsic affinity of this antibody was measured as KD = 0.3 nM by real time, label free molecular interaction analysis on immobilized panitumumab.
      • Approx. Protein Concentrations
        Antibody concentration 0.5 mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        12 months from date of despatch
      • Acknowledgements
        Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany, a wholly owned subsidiary of Bio-Rad Laboratories Inc.
        Vectibix® is a registered trademark of Amgen.
      • Licensed Use
        For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        ELISA

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
        Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
      • Recommended Protocol
      • ELISA
        This product may be used in direct or indirect ELISA and in an ELISA bridging assay as a capture antibody together with HCA265 or HCA266 as the detection reagent when conjugated to a suitable label, e.g. HRP.
        Protocol: PK bridging ELISA to measure free drug
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use

      Additional Panitumumab Antibody Formats

      Formats Clone Applications Sizes available
      Panitumumab Antibody : Purified AbD23894 E 0.1 mg
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Rat anti DYKDDDDK Tag:HRPMCA4764P0.1 mgE, P, WB
        MCA4764P
        Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
        0500-0099
        Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
        MCA5995P

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Human anti PanitumumabHCA2650.1 mgE
            HCA265
            Human anti PanitumumabHCA2660.1 mgE
            HCA266
            Hispec Assay DiluentBUF049A50 mlIY
            BUF049A

            Recommended Positive Controls

              Histology Controls

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