Trem-2 antibody | 78.18
Rat anti Mouse TREM-2 antibody, clone 78.18 (MCA4772EL) used as a blocking reagent on primary microglia to look at the interaction between TREM2 and Aβ monomers.
Image caption:
Full-length TREM2 binds Aβ oligomers but not monomers.
i) Primary microglia were incubated with 100 nM HiLyte Fluor 647-labelled Aβ42 at 4°C for 1 hour, then lysed, immunoprecipitated with anti-TREM2 antibodies, and examined by SDS-PAGE and western blotting. Aβ oligomers co-immunoprecipitate with endogenous TREM2 in primary microglia (lane 2), but not with other natively endogenous Type I glycoproteins, such as nicastrin (lane 4) or with isotype control antibody (lane 3). TREM2 knockout microglia failed to co-immunoprecipitate Aβ (lane 1). ii) This interaction was blocked by preincubation with monoclonal anti-TREM2 blocking antibody (lane 2), but not by isotype control antibodies (lane 3).
From: Vilalta A, Zhou Y, Sevalle J, Griffin JK, Satoh K, Allendorf DH, De S, Puigdellívol M, Bruzas A, Burguillos MA, Dodd RB, Chen F, Zhang Y, Flagmeier P, Needham LM, Enomoto M, Qamar S, Henderson J, Walter J, Fraser PE, Klenerman D, Lee SF, St George-Hyslop P, Brown GC.
Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.
J Biol Chem. 2021 Apr 3;296:100631.
doi: 10.1016/j.jbc.2021.100631.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Trem-2:Alexa Fluor® 647
Rat anti Mouse Trem-2:FITC
Rat anti Mouse Trem-2:Low Endotoxin
Rat anti Mouse Trem-2
Rat anti Mouse Trem-2:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 78.18
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA4772A647 | F | 100 Tests/1ml |
|
|
| MCA4772F | F | 0.1 mg |
|
|
| MCA4772EL | F FN | 0.5 mg |
|
|
| MCA4772GA | F | 0.1 mg |
|
|
| MCA4772 | F | 0.25 mg |
|
|
| MCA4772PE | F | 100 Tests |
|
Rat anti Mouse TREM-2 antibody, clone 78.18 recognizes triggering receptor expressed on myeloid cells 2 (TREM-2), an activating receptor of the Ig superfamily. TREM-2 forms a receptor signaling complex with DAP12 to trigger the activation of the immune response in macrophages and dendritic cells. It may be involved in chronic inflammation and may promote the production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 may also have a functional role in osteoclast formation.
Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Rat anti Mouse Trem-2 antibody, clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts.
Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Rat anti Mouse Trem-2 antibody, clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1ml distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Mouse TREM-2 Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised Fisher rats were fused with cells of the rat YB-2/0 myeloma cell line
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q99NH8
- Entrez Gene
- Trem2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale of for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
- Acknowledgements
- This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale of for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
- Acknowledgements
- This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale of for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
- Acknowledgements
- This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale of for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
- Regulatory
- For research purposes only
Applications of Trem-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | |||
| Flow Cytometry | 1/25 | 1/200 | |
| Functional Assays | |||
| Flow Cytometry | 1/25 | 1/200 | |
| Flow Cytometry | Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | ||
| Human Seroblock | BUF070B | F | 200 Test | ||
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
|
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
|
Product Specific References
References for Trem-2 antibody
-
Humphrey, M.B. et al. (2006) TREM2, a DAP12-associated receptor, regulates osteoclast differentiation and function.
J Bone Miner Res. 21 (2): 237-45. -
Vilalta, A. et al. (2021) Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.
J Biol Chem. 296:100631.
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