Trem-2 antibody | 78.18
Rat anti Mouse TREM-2 antibody, clone 78.18 (MCA4772EL) used as a blocking reagent on primary microglia to look at the interaction between TREM2 and Aβ monomers.
Image caption:
Full-length TREM2 binds Aβ oligomers but not monomers.
i) Primary microglia were incubated with 100 nM HiLyte Fluor 647-labelled Aβ42 at 4°C for 1 hour, then lysed, immunoprecipitated with anti-TREM2 antibodies, and examined by SDS-PAGE and western blotting. Aβ oligomers co-immunoprecipitate with endogenous TREM2 in primary microglia (lane 2), but not with other natively endogenous Type I glycoproteins, such as nicastrin (lane 4) or with isotype control antibody (lane 3). TREM2 knockout microglia failed to co-immunoprecipitate Aβ (lane 1). ii) This interaction was blocked by preincubation with monoclonal anti-TREM2 blocking antibody (lane 2), but not by isotype control antibodies (lane 3).
From: Vilalta A, Zhou Y, Sevalle J, Griffin JK, Satoh K, Allendorf DH, De S, Puigdellívol M, Bruzas A, Burguillos MA, Dodd RB, Chen F, Zhang Y, Flagmeier P, Needham LM, Enomoto M, Qamar S, Henderson J, Walter J, Fraser PE, Klenerman D, Lee SF, St George-Hyslop P, Brown GC.
Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.
J Biol Chem. 2021 Apr 3;296:100631.
doi: 10.1016/j.jbc.2021.100631.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Trem-2
- Product Type
- Monoclonal Antibody
- Clone
- 78.18
- Isotype
- IgG1
- Specificity
- Trem-2
Antibody Quality and Performance Guarantee
| Rat anti Mouse TREM-2 antibody, clone 78.18 recognizes triggering receptor expressed on myeloid cells 2 (TREM-2), an activating receptor of the Ig superfamily. TREM-2 forms a receptor signaling complex with DAP12 to trigger the activation of the immune response in macrophages and dendritic cells. It may be involved in chronic inflammation and may promote the production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 may also have a functional role in osteoclast formation. Stimulation of TREM-2 with Rat anti Mouse Trem-2 antibody, clone 78.18 enhances multinucleated osteoclast formation in vitro. Rat anti Mouse Trem-2 antibody, clone 78.18 may also be used to block TREM-2 on pre-osteoclasts to inhibit the formation of multinuclear osteoclasts and to inhibit bone resorption by mature osteoclasts. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Mouse TREM-2 Fc fusion protein
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised Fisher rats were fused with cells of the rat YB-2/0 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale of for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for Trem-2 antibody
-
Humphrey, M.B. et al. (2006) TREM2, a DAP12-associated receptor, regulates osteoclast differentiation and function.
J Bone Miner Res. 21 (2): 237-45. -
Vilalta, A. et al. (2021) Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.
J Biol Chem. 296:100631.
- RRID
- AB_10845148
- UniProt
- Q99NH8
- Entrez Gene
- Trem2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- GO:0004872 receptor activity
MCA4772
162735
1805
MCA4772GA
156964
1806
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