TNF Alpha antibody | MP6-XT22

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1ug/ml	10ug/ml
Flow Cytometry 1		1/10	1/50
Immunohistology - Frozen

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl

References for TNF Alpha antibody

1. Yang, J. et al. (1995) Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG.
   J Immunol. 155 (12): 5728-35.
2. Ross, S.E. et al. (1997) Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram.
   J Immunol. 159 (12): 6253-9.
3. Lee, Y.L. et al. (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma 180 inoculation model.
   Exp Anim. 52: 371-5.
4. Ramakrishna, C. et al. (2004) Differential regulation of primary and secondary CD8+ T cells in the central nervous system.
   J Immunol. 173: 6265-73.
5. Jordan, M.B. et al. (2004) An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder.
   Blood. 104: 735-43.
6. Hanada, T. et al. (2006) IFNgamma-dependent, spontaneous development of colorectal carcinomas in SOCS1-deficient mice.
   J Exp Med. 203: 1391-7.
7. Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
   J Exp Med. 204: 3037-47.
8. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
   Am J Pathol. 173: 119-29.
View The Latest Product References
9. Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
   Curr Alzheimer Res. 6: 531-40.
10. O'Dea, K.P. et al. (2011) Regulation of monocyte subset proinflammatory responses within the lung microvasculature by the p38 MAPK/MK2 pathway.
    Am J Physiol Lung Cell Mol Physiol. 301: L812-21.
11. Lee JK et al. (2015) Iron accumulation promotes TACE-mediated TNF-α secretion and neurodegeneration in a mouse model of ALS.
    Neurobiol Dis. 80: 63-9.
12. Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
    Biol Trace Elem Res. 164 (2): 205-11.
13. Hirose, M. et al. (2017) Reduced skin blistering in experimental epidermolysis bullosa acquisita after anti-TNF treatment.
    Mol Med. 22: 918-926.
14. Yan, J. et al. (2018) Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.
    Nat Commun. 9 (1): 33.
15. Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
    Int J Morphol., 38 (3): 804-10,
16. Mohammadrezaei, R.K. et al. (2020) Quercetin postconditioning attenuates gastrocnemius muscle ischemia/reperfusion injury in rats.
    J Cell Physiol. 235 (12): 9876-9883.

Further Reading

1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

RRID

AB_323519

UniProt

P06804

Entrez Gene

Tnf

GO Terms

GO:0000122 negative regulation of transcription from RNA polymerase II promoter

GO:0001891 phagocytic cup

GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus

GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin

GO:0005887 integral to plasma membrane

GO:0005125 cytokine activity

GO:0005164 tumor necrosis factor receptor binding

GO:0005615 extracellular space

GO:0030141 stored secretory granule

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GO:0006006 glucose metabolic process

GO:0006927 transformed cell apoptosis

GO:0006959 humoral immune response

GO:0007254 JNK cascade

GO:0009887 organ morphogenesis

GO:0050830 defense response to Gram-positive bacterium

GO:0008625 induction of apoptosis via death domain receptors

GO:0009897 external side of plasma membrane

GO:0030198 extracellular matrix organization

GO:0030316 osteoclast differentiation

GO:0031622 positive regulation of fever generation

GO:0032729 positive regulation of interferon-gamma production

GO:0032755 positive regulation of interleukin-6 production

GO:0033209 tumor necrosis factor-mediated signaling pathway

GO:0044130 negative regulation of growth of symbiont in host

GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade

GO:0045121 membrane raft

GO:0045670 regulation of osteoclast differentiation

GO:0045944 positive regulation of transcription from RNA polymerase II promoter

GO:0045994 positive regulation of translational initiation by iron

GO:0046325 negative regulation of glucose import

GO:0046330 positive regulation of JNK cascade

GO:0051023 regulation of immunoglobulin secretion

GO:0051798 positive regulation of hair follicle development

GO:0055037 recycling endosome

GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis

GO:0060693 regulation of branching involved in salivary gland morphogenesis

MCA1488

0814 158108

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