TNF Alpha antibody | MP6-XT22
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Rat anti Mouse TNF Alpha
- Product Type
- Monoclonal Antibody
- TNF Alpha
|Rat anti Mouse TNF alpha antibody, clone MP6-XT22 recognizes mouse tumor necrosis factor alpha (TNF alpha), a ~17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a ~26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.
Rat anti Mouse TNF alpha antibody, clone MP6-XT22 is reported to neutralize the effects of mouse TNF alpha (Gaup et al. 2008 and Yang et al. 1995).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Recombinant murine TNF alpha expressed in E. coli.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/10||1/50|
|Immunohistology - Frozen|
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for TNF Alpha antibody
Yang, J. et al. (1995) Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG.
J Immunol. 155 (12): 5728-35.
Ross, S.E. et al. (1997) Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram.
J Immunol. 159 (12): 6253-9.
Lee, Y.L. et al. (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma 180 inoculation model.
Exp Anim. 52: 371-5.
Ramakrishna, C. et al. (2004) Differential regulation of primary and secondary CD8+ T cells in the central nervous system.
J Immunol. 173: 6265-73.
Jordan, M.B. et al. (2004) An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder.
Blood. 104: 735-43.
Hanada, T. et al. (2006) IFNgamma-dependent, spontaneous development of colorectal carcinomas in SOCS1-deficient mice.
J Exp Med. 203: 1391-7.
Nahrendorf, M. et al. (2007) The healing myocardium sequentially mobilizes two monocyte subsets with divergent and complementary functions.
J Exp Med. 204: 3037-47.
Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
Am J Pathol. 173: 119-29.
View The Latest Product References
Ruan, L. et al. (2009) Amyloid deposition and inflammation in APPswe/PS1dE9 mouse model of Alzheimer's disease.
Curr Alzheimer Res. 6: 531-40.
O'Dea, K.P. et al. (2011) Regulation of monocyte subset proinflammatory responses within the lung microvasculature by the p38 MAPK/MK2 pathway.
Am J Physiol Lung Cell Mol Physiol. 301: L812-21.
Lee JK et al. (2015) Iron accumulation promotes TACE-mediated TNF-α secretion and neurodegeneration in a mouse model of ALS.
Neurobiol Dis. 80: 63-9.
Gholami, M. et al. (2015) Selenium effect on ischemia-reperfusion injury of gastrocnemius muscle in adult rats.
Biol Trace Elem Res. 164 (2): 205-11.
Hirose, M. et al. (2017) Reduced skin blistering in experimental epidermolysis bullosa acquisita after anti-TNF treatment.
Mol Med. 22: 918-926.
Yan, J. et al. (2018) Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.
Nat Commun. 9 (1): 33.
Gholami, M.R. et al. (2020) Protective effects of honey, Apis mellifera meda. skorikov, on ischemia-reperfusion induced muscle injury.
Int J Morphol., 38 (3): 804-10,
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
- Entrez Gene
- GO Terms
- GO:0000122 negative regulation of transcription from RNA polymerase II promoter
- GO:0001891 phagocytic cup
- GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus
- GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0030141 stored secretory granule
- View More GO Terms
- GO:0006006 glucose metabolic process
- GO:0006927 transformed cell apoptosis
- GO:0006959 humoral immune response
- GO:0007254 JNK cascade
- GO:0009887 organ morphogenesis
- GO:0050830 defense response to Gram-positive bacterium
- GO:0008625 induction of apoptosis via death domain receptors
- GO:0009897 external side of plasma membrane
- GO:0030198 extracellular matrix organization
- GO:0030316 osteoclast differentiation
- GO:0031622 positive regulation of fever generation
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0033209 tumor necrosis factor-mediated signaling pathway
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045121 membrane raft
- GO:0045670 regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0045994 positive regulation of translational initiation by iron
- GO:0046325 negative regulation of glucose import
- GO:0046330 positive regulation of JNK cascade
- GO:0051023 regulation of immunoglobulin secretion
- GO:0051798 positive regulation of hair follicle development
- GO:0055037 recycling endosome
- GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
- GO:0060693 regulation of branching involved in salivary gland morphogenesis
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