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Kappa Light Chain antibody | OX-20

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Anti Mouse Kappa Light Chain Antibody, clone OX-20 thumbnail image 1 Anti Mouse Kappa Light Chain Antibody, clone OX-20 thumbnail image 2 Anti Mouse Kappa Light Chain Antibody, clone OX-20 thumbnail image 3 Anti Mouse Kappa Light Chain Antibody, clone OX-20 thumbnail image 4
Anti Mouse Kappa Light Chain Antibody, clone OX-20 gallery image 1
Purified Mouse IgG (PMP01) used as coating reagent in a direct ELISA detected with Rat anti Mouse Kappa Light Chain:HRP (MCA152P).
Anti Mouse Kappa Light Chain Antibody, clone OX-20 gallery image 2
A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 5. 1, 0.5 and 0.25 ug of antibody were run in lanes 2, 3 and 4 and 6, 7 and 8 respectively. Protein detection was carried out on lanes 2, 3 and 4 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 6, 7 and 8 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure time. STAR207P is specific for both the heavy (50kDa) and light (25kDa) chains whereas MCA152P is specific for the light chains.
Anti Mouse Kappa Light Chain Antibody, clone OX-20 gallery image 3
HeLa (lanes 2, 5 and 8) and HepG2 (lanes 3, 6 and 9) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1, 4 and 7. A Mouse anti Human GAPDH (MCA4740) was used as the primary antibody at a dilution of 1/1000. Rat anti Mouse Kappa Light Chain:HRP (MCA152P) was used as the secondary antibody at 1/1000 (lanes 2 and 3), 1/5000 (lanes 5 and 6) and 1/10000 (lanes 8 and 9). Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure.
Anti Mouse Kappa Light Chain Antibody, clone OX-20 gallery image 4
A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 Western Workflow™ and transferred to a PVDF membrane. Precision Plus Protein™ Prestained Standards were run in lane 1.

1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc™ Touch Imaging System.

Rat anti Mouse Kappa Light Chain:HRP

Rat anti Mouse Kappa Light Chain

Product Type
Monoclonal Antibody
Clone
OX-20
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA152P
Datasheet
E WB
SDS
0.1 mg loader

MCA152
Datasheet
F
SDS
1 mg loader

Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified mouse IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunized PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01837
Entrez Gene
Igk-C
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of Kappa Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/10,000
Western Blotting 1/1000 1/10,000
Flow Cytometry 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Product Specific References

References for Kappa Light Chain antibody

  1. Mason, D.W. et al. (1981) The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies.
    Immunology. 44 (1): 75-87.
  2. White, R.A. et al. (1984) Differentiation markers in the Drosophila ovary.
    J Embryol Exp Morphol. 84: 275-86.
  3. Meyer, K.B. et al. (1990) The importance of the 3'-enhancer region in immunoglobulin kappa gene expression.
    Nucleic Acids Res. 18 (19): 5609-15.
  4. Thomas, M.J. et al. (1999) CD8 T-lymphocyte-mediated regulation of ovalbumin-specific murine IgE responses.
    Int Arch Allergy Immunol. 118 (2-4): 289-91.
  5. Wasserman, G.M. et al. (1993) An approach to development of specific T-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria.
    Infect Immun. 61 (5): 1958-63.
  6. Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
    J Immunol. 168 (1): 216-23.
  7. Salagianni, M. et al. (2007) An essential role for IL-18 in CD8 T cell-mediated suppression of IgE responses.
    J Immunol. 178 (8): 4771-8.

Further Reading

  1. Galfrè, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.
    Nature. 277 (5692): 131-3.

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