Kappa Light Chain antibody | OX-20
1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 4 and 5 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Detection was carried out on lanes 6 and 7 using Goat anti Mouse IgG (Fc):HRP (STAR120P) at a dilution of 1/5,000. Visualization was carried out using the Chemidoc Touch Imaging System.
Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Purified mouse IgG.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from an immunized PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01837
- Entrez Gene
- Igk-C
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of Kappa Light Chain antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/100 | 1/10,000 | |
| Western Blotting | 1/1000 | 1/10,000 | |
| Flow Cytometry | 1/100 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Kappa Light Chain antibody
-
Mason, D.W. et al. (1981) The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies.
Immunology. 44 (1): 75-87. -
White, R.A. et al. (1984) Differentiation markers in the Drosophila ovary.
J Embryol Exp Morphol. 84: 275-86. -
Meyer, K.B. et al. (1990) The importance of the 3'-enhancer region in immunoglobulin kappa gene expression.
Nucleic Acids Res. 18 (19): 5609-15. -
Thomas, M.J. et al. (1999) CD8 T-lymphocyte-mediated regulation of ovalbumin-specific murine IgE responses.
Int Arch Allergy Immunol. 118 (2-4): 289-91. -
Wasserman, G.M. et al. (1993) An approach to development of specific T-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria.
Infect Immun. 61 (5): 1958-63. -
Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
J Immunol. 168 (1): 216-23. -
Salagianni, M. et al. (2007) An essential role for IL-18 in CD8 T cell-mediated suppression of IgE responses.
J Immunol. 178 (8): 4771-8. -
Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
Mol Ther. 29 (3): 1174-85.
Further Reading
-
Galfrè, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.
Nature. 277 (5692): 131-3.
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