Kappa Light Chain antibody | OX-20

Rat anti Mouse Kappa Light Chain

Product Type
Monoclonal Antibody
Clone
OX-20
Isotype
IgG2b
Specificity
Kappa Light Chain

Product Code Applications Pack Size List Price Your Price Qty
MCA152
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Purified mouse IgG.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from an immunized PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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References for Kappa Light Chain antibody

  1. Mason, D.W. et al. (1981) The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies.
    Immunology. 44 (1): 75-87.
  2. White, R.A. et al. (1984) Differentiation markers in the Drosophila ovary.
    J Embryol Exp Morphol. 84: 275-86.
  3. Meyer, K.B. et al. (1990) The importance of the 3'-enhancer region in immunoglobulin kappa gene expression.
    Nucleic Acids Res. 18 (19): 5609-15.
  4. Thomas, M.J. et al. (1999) CD8 T-lymphocyte-mediated regulation of ovalbumin-specific murine IgE responses.
    Int Arch Allergy Immunol. 118 (2-4): 289-91.
  5. Wasserman, G.M. et al. (1993) An approach to development of specific T-lymphocyte lines by use of preprocessed antigens in Plasmodium vinckei vinckei murine malaria.
    Infect Immun. 61 (5): 1958-63.
  6. Thomas, M.J. et al. (2002) CD8 T cells inhibit IgE via dendritic cell IL-12 induction that promotes Th1 T cell counter-regulation.
    J Immunol. 168 (1): 216-23.
  7. Salagianni, M. et al. (2007) An essential role for IL-18 in CD8 T cell-mediated suppression of IgE responses.
    J Immunol. 178 (8): 4771-8.
  8. Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
    Mol Ther. 29 (3): 1174-85.
  9. View The Latest Product References

Further Reading

  1. Galfrè, G. et al. (1979) Rat x rat hybrid myelomas and a monoclonal anti-Fd portion of mouse IgG.
    Nature. 277 (5692): 131-3.

RRID
AB_321880
UniProt
P01837
Entrez Gene
Igk-C
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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