IL-5 antibody | TRFK5

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1
Western Blotting

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Source Reference

1. Schumacher, J.H. et al. (1988) The characterization of four monoclonal antibodies specific for mouse IL-5 and development of mouse and human IL-5 enzyme-linked immunosorbent.
   J Immunol. 141 (5): 1576-81.

References for IL-5 antibody

1. Betz, M. & Fox, B.S. (1990) Regulation and development of cytochrome c-specific IL-4-producing T cells.
   J Immunol. 145 (4): 1046-52.
2. Röcken, M. et al. (1992) Central role for TCR/CD3 ligation in the differentiation of CD4+ T cells toward A Th1 or Th2 functional phenotype.
   J Immunol. 148 (1): 47-54.
3. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
4. Portanova, J.P. et al. (1995) Rapid and selective induction of blood eosinophilia in guinea pigs by recombinant human interleukin 5.
   Cytokine. 7 (8): 775-83.
5. Akdis, C.A. et al. (1996) Epitope-specific T cell tolerance to phospholipase A2 in bee venom immunotherapy and recovery by IL-2 and IL-15 in vitro.
   J Clin Invest. 98 (7): 1676-83.
6. Dawson, H.D. et al. (2020) Porcine cytokines, chemokines and growth factors: 2019 update.
   Res Vet Sci. 131: 266-300.

RRID

AB_2264942

UniProt

P04401

Entrez Gene

Il5

GO Terms

GO:0005125 cytokine activity

GO:0005615 extracellular space

GO:0006955 immune response

GO:0008083 growth factor activity

GO:0043193 positive regulation of gene-specific transcription

GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation

GO:0051024 positive regulation of immunoglobulin secretion

GO:0051091 positive regulation of transcription factor activity

MCA1495

170801 180301 180302

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