IL-4 antibody | BVD4-1D11

100% Secure


Rat anti Mouse Interleukin-4

Product Type
Monoclonal Antibody
Clone
BVD4-1D11
Isotype
IgG2b
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1304G C E F* WB 0.5 mg
Rat anti Mouse Interleukin-4 antibody, clone BVD4-1D11 recognizes natural and recombinant mouse IL-4. 99% pure by SDS-PAGE.

Clone BVD4-1D11 is reported to neutralise IL-4 activity (Abrams et al., 1992).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant Mouse IL-4.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P07750 Related reagents
Entrez Gene
Il4 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005136 interleukin-4 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0008203 cholesterol metabolic process
GO:0030890 positive regulation of B cell proliferation
GO:0042104 positive regulation of activated T cell proliferation
GO:0042113 B cell activation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0043066 negative regulation of apoptosis
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045671 negative regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0048295 positive regulation of isotype switching to IgE isotypes
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0051091 positive regulation of transcription factor activity
Regulatory
For research purposes only

Applications of IL-4 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 5ug/ml
Flow Cytometry 1 1/10 1/50
Immunohistology - Frozen
Western Blotting 1ug/ml 5ug/ml
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Application Based External Images

Functional Assays

Product Specific References

References for IL-4 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
    J Immunol Methods. 166 (2): 201-14.
  3. Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
    J Exp Med. 192 (6): 823-33.
  4. Pechova, J. et al. (2004) Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells.
    Folia Parasitol (Praha). 51: 367-72.
  5. Finkelman, F.D. et al. (2000) Stat6 regulation of in vivo IL-4 responses.
    J Immunol. 164: 2303-10.
  6. Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
    J Exp Med. 192: 823-33.
  7. Urban, J.F. Jr. et al. (2000) Stat6 signaling promotes protective immunity against Trichinella spiralis through a mast cell- and T cell-dependent mechanism.
    J Immunol. 164: 2046-52.
  8. Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
    Int Immunol. 12: 1669-75.
  9. Pechová, J. et al. (2004) Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells.
    Folia Parasitol (Praha). 51: 367-72.
  10. Roy, E. et al. (2008) Beneficial effect of anti-interleukin-4 antibody when administered in a murine model of tuberculosis infection.
    Tuberculosis (Edinb). 88: 197-202.