IL-4 antibody | BVD4-1D11
Filter by Application:
WB ResetRat anti Mouse Interleukin-4
- Product Type
- Monoclonal Antibody
- Clone
- BVD4-1D11
- Isotype
- IgG2b
- Specificity
- IL-4
Antibody Quality and 
Unrivaled 
| Rat anti Mouse Interleukin-4 antibody, clone BVD4-1D11 recognizes natural and recombinant mouse IL-4. Clone BVD4-1D11 is reported to neutralize IL-4 activity (Abrams et al., 1992). |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Recombinant Mouse IL-4.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1ug/ml | 5ug/ml |
| Flow Cytometry 1 | |
1/10 | 1/50 |
| Immunohistology - Frozen | |
||
| Western Blotting | |
1ug/ml | 5ug/ml |
- 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for IL-4 antibody
-
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. -
Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
J Immunol Methods. 166 (2): 201-14. -
Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
J Exp Med. 192 (6): 823-33. -
Pechová, J. et al. (2004) Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells.
Folia Parasitol (Praha). 51: 367-72. -
Finkelman, F.D. et al. (2000) Stat6 regulation of in vivo IL-4 responses.
J Immunol. 164: 2303-10. -
Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
J Exp Med. 192: 823-33. -
Urban, J.F. Jr. et al. (2000) Stat6 signaling promotes protective immunity against Trichinella spiralis through a mast cell- and T cell-dependent mechanism.
J Immunol. 164: 2046-52. -
Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
Int Immunol. 12: 1669-75.
View The Latest Product References
-
Roy, E. et al. (2008) Beneficial effect of anti-interleukin-4 antibody when administered in a murine model of tuberculosis infection.
Tuberculosis (Edinb). 88 (3): 197-202.
- RRID
- AB_322398
- UniProt
- P07750
- Entrez Gene
- Il4
- GO Terms
- GO:0005125 cytokine activity
- GO:0005136 interleukin-4 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0008203 cholesterol metabolic process
- GO:0030890 positive regulation of B cell proliferation
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042113 B cell activation
- View More GO Terms
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0043066 negative regulation of apoptosis
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0045671 negative regulation of osteoclast differentiation
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0048295 positive regulation of isotype switching to IgE isotypes
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0051091 positive regulation of transcription factor activity
View more products with IL-4 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up