IL-2 antibody | JES6-5H4
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Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 recognizes natural and recombinant murine IL-2.
Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 does not function as a blocking antibody (Sander et al. 1993). For neutralization of murine IL-2 we recommend the use of Rat anti Mouse IL-2 antibody, clone JES6-1A12 (MCA1493).
Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 does not function as a blocking antibody (Sander et al. 1993). For neutralization of murine IL-2 we recommend the use of Rat anti Mouse IL-2 antibody, clone JES6-1A12 (MCA1493).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Immunogen
- Recombinant Mouse IL-2.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 12 months from date of reconstitution.
More Information
- UniProt
- P04351 Related reagents
- Entrez Gene
- Il2 Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005134 interleukin-2 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0042104 positive regulation of activated T cell proliferation
- GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
- GO:0045944 positive regulation of transcription from RNA polymerase II promoter
- GO:0046013 regulation of T cell homeostatic proliferation
- GO:0048304 positive regulation of isotype switching to IgG isotypes
- GO:0050672 negative regulation of lymphocyte proliferation
- GO:0050728 negative regulation of inflammatory response
- GO:0051024 positive regulation of immunoglobulin secretion
- Regulatory
- For research purposes only
Applications of IL-2 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 | Neat | 1/10 | |
| ELISA | |||
| Flow Cytometry 1 | 1/10 | 1/50 | |
| Immunoprecipitation | |||
| Flow Cytometry 1 | Neat | 1/10 |
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F |
Product Specific References
References for IL-2 antibody
-
Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
J Biol Chem. 275 (27): 20288-94. -
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24. -
Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
Eur J Immunol. 45 (2): 480-91.
