IL-2 antibody | JES6-5H4

Rat anti Mouse Interleukin-2

Product Type
Monoclonal Antibody
Clone
JES6-5H4
Isotype
IgG2b
Specificity
IL-2

Product Code Applications Pack Size List Price Your Price Qty
MCA1303G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * IP 0.5 mg loader
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loader
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Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 recognizes natural and recombinant murine IL-2.

Rat anti Mouse Interleukin-2 antibody, clone JES6-5H4 does not function as a blocking antibody (Sander et al. 1993). For neutralization of murine IL-2 we recommend the use of Rat anti Mouse IL-2 antibody, clone JES6-1A12 (MCA1493).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant Mouse IL-2.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry 1 1/10 1/50
Immunoprecipitation
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

References for IL-2 antibody

  1. Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
    J Biol Chem. 275 (27): 20288-94.
  2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  3. Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
    Eur J Immunol. 45 (2): 480-91.

RRID
AB_2123680
UniProt
P04351
Entrez Gene
Il2
GO Terms
GO:0005125 cytokine activity
GO:0005134 interleukin-2 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0042104 positive regulation of activated T cell proliferation
GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046013 regulation of T cell homeostatic proliferation
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050672 negative regulation of lymphocyte proliferation
GO:0050728 negative regulation of inflammatory response
GO:0051024 positive regulation of immunoglobulin secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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