IL-2 antibody | JES6-5H4

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry 1		1/10	1/50
Immunoprecipitation

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

References for IL-2 antibody

1. Tachibana, M. et al. (2000) Coreceptor function of mutant human CD4 molecules without affinity to gp120 of human immunodeficiency virus.
   J Biol Chem. 275 (27): 20288-94.
2. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
3. Pletinckx, K. et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
   Eur J Immunol. 45 (2): 480-91.

RRID

AB_2123680

UniProt

P04351

Entrez Gene

Il2

GO Terms

GO:0005125 cytokine activity

GO:0005134 interleukin-2 receptor binding

GO:0005615 extracellular space

GO:0006955 immune response

GO:0008083 growth factor activity

GO:0042104 positive regulation of activated T cell proliferation

GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein

GO:0045944 positive regulation of transcription from RNA polymerase II promoter

GO:0046013 regulation of T cell homeostatic proliferation

View More GO Terms

GO:0048304 positive regulation of isotype switching to IgG isotypes

GO:0050672 negative regulation of lymphocyte proliferation

GO:0050728 negative regulation of inflammatory response

GO:0051024 positive regulation of immunoglobulin secretion

MCA1303G

1511 158127 1612

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