IL-10 antibody | JES5-2A5

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/200	1/2000
Flow Cytometry 1		1/10	1/100
Immunohistology - Frozen
Western Blotting			1/1000

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Source Reference

1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunological Review 127: 5-24.

References for IL-10 antibody

1. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
   J Immunol Methods. 166 (2): 201-14.
2. Nishigori, C. et al. (1998) HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.
   J Immunol. 161 (6): 2684-91.
3. Weber-nordt, R.M. et al. (1994) Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.
   J Immunol. 153 (8): 3734-44.
4. Rennick, D. et al. (1995) Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts.
   Blood. 85: 57-65.
5. Biswas, S.K. et al. (2006) A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
   Blood. 107: 2112-22.
6. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
   Am J Pathol. 173: 119-29.
7. Gonzalez-Juarrero, M. et al. (2013) Polar Lipids of Burkholderia pseudomallei Induce Different Host Immune Responses.
   PLoS One. 8: e80368.
8. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
   Vet Immunol Immunopathol. 202: 74-84.
View The Latest Product References
9. Hammond, J.W. et al. (2018) HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q.
   Glia. 66 (12): 2563-74.

Flow Cytometry

Immunohistology - Frozen

Western Blotting

RRID

AB_2125093

UniProt

P18893

Entrez Gene

Il10

GO Terms

GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus

GO:0042742 defense response to bacterium

GO:0005125 cytokine activity

GO:0005615 extracellular space

GO:0030886 negative regulation of myeloid dendritic cell activation

GO:0032689 negative regulation of interferon-gamma production

GO:0032695 negative regulation of interleukin-12 production

GO:0032720 negative regulation of tumor necrosis factor production

GO:0044130 negative regulation of growth of symbiont in host

View More GO Terms

GO:0043193 positive regulation of gene-specific transcription

GO:0045348 positive regulation of MHC class II biosynthetic process

GO:0060670 branching involved in embryonic placenta morphogenesis

MCA1302G

154985 156905 158998 1708

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