IL-10 antibody | JES5-2A5

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Anti Mouse Interleukin-10 Antibody, clone JES5-2A5 gallery image 1 — Western blot analysis of mouse IL-10 recombinant protein probed with Rat anti Mouse Interleukin-10 (**MCA1302G**) followed by F(ab')₂ Rabbit anti Rat IgG:HRP (STAR21B)

Rat anti Mouse Interleukin-10

Product Type: Monoclonal Antibody
Clone: JES5-2A5
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
MCA1302G Datasheet	C E F* WB SDS	0.5 mg	Log in		Get Quote

Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells (O'Garra et al. 1992) and a subset of stimulated T cells (Asseman et al. 1999).

IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.

Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordt et al. 1994).

Product Details

Target Species

Mouse

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Recombinant Mouse IL-10.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P18893
Entrez Gene: Il10
GO Terms: GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus; GO:0042742 defense response to bacterium; GO:0005125 cytokine activity; GO:0005615 extracellular space; GO:0030886 negative regulation of myeloid dendritic cell activation; GO:0032689 negative regulation of interferon-gamma production; GO:0032695 negative regulation of interleukin-12 production; GO:0032720 negative regulation of tumor necrosis factor production; GO:0044130 negative regulation of growth of symbiont in host; GO:0043193 positive regulation of gene-specific transcription; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0060670 branching involved in embryonic placenta morphogenesis
Regulatory: For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/200	1/2000
Flow Cytometry ¹	1/10	1/100
Immunohistology - Frozen
Western Blotting		1/1000

¹ Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)	STAR131A	C E P WB	1 ml	Log in	Get Quote
Goat anti Rat IgG:Biotin (Mouse Adsorbed)	STAR131B	C E IF P WB	0.5 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:Dylight®800	STAR16D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:FITC	STAR17B	F	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Rat IgG:HRP	STAR21B	C E P RE	1 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)	STAR69	F	0.5 ml	Log in	Get Quote
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)	STAR71D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)	STAR71D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Rat IgG:HRP (Mouse Adsorbed)	STAR72	C E P	0.5 mg	Log in	Get Quote
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)	STAR73	F	0.5 ml	Log in	Get Quote

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Western Blotting

Product Specific References

References for IL-10 antibody

Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunological Review 127: 5-24.
Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
J Immunol Methods. 166 (2): 201-14.
Nishigori, C. et al. (1998) HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.
J Immunol. 161 (6): 2684-91.
Weber-nordt, R.M. et al. (1994) Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.
J Immunol. 153 (8): 3734-44.
Rennick, D. et al. (1995) Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts.
Blood. 85: 57-65.
Biswas, S.K. et al. (2006) A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
Blood. 107: 2112-22.
Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
Am J Pathol. 173: 119-29.
Gonzalez-Juarrero, M. et al. (2013) Polar Lipids of Burkholderia pseudomallei Induce Different Host Immune Responses.
PLoS One. 8: e80368.
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84.
Hammond, J.W. et al. (2018) HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q.
Glia. 66 (12): 2563-74.

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