IL-10 antibody | JES5-2A5

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Rat anti Mouse Interleukin-10

Product Type
Monoclonal Antibody
Clone
JES5-2A5
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1302G C E F* WB 0.5 mg
Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5  recognizes mouse Cytokine synthesis inhibitory factor (CSIF), also known as interleukin-10 (IL-10). IL-10 is produced by Ly-1(CD5) expressing B cells (O'Garra et al. 1992) and a subset of stimulated T cells (Asseman et al. 1999).

IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.

Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordt et al. 1994).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Recombinant Mouse IL-10.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P18893 Related reagents
Entrez Gene
Il10 Related reagents
GO Terms
GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
GO:0042742 defense response to bacterium
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0030886 negative regulation of myeloid dendritic cell activation
GO:0032689 negative regulation of interferon-gamma production
GO:0032695 negative regulation of interleukin-12 production
GO:0032720 negative regulation of tumor necrosis factor production
GO:0044130 negative regulation of growth of symbiont in host
GO:0043193 positive regulation of gene-specific transcription
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0060670 branching involved in embryonic placenta morphogenesis
Regulatory
For research purposes only

Applications of IL-10 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/200 1/2000
Flow Cytometry 1 1/10 1/100
Immunohistology - Frozen
Western Blotting 1/1000
  1. 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control MCA1211 1 ml C E F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

References for IL-10 antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunological Review 127: 5-24.
  2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining
    J. Immunol. Methods. 166: 201-14.
  3. Guesdon, J.L. et al. (1979) The use of avidin-biotin interaction in immunoenzymatic techniques.
    J. Histochem. Cytochem. 27: 1131-1139.
  4. Nishigori, C. et al. (1998) Hind III liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.
    J. Immunol. 161:2684-2691.
  5. Weber-Nordt, R. et al. (1994) Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.
    J. Immunol. 153:3734-3744.
  6. Rennick, D. et al. (1995) Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts.
    Blood. 85: 57-65.
  7. Sacco, S. et al. (1997) Protective effect of a single Interleukin-12 (IL-12) predose against the toxicity of subsequent chronic IL-12 in mice: Role of cytokines and glucocorticoids.
    Blood. 90:4473-4479.
  8. Biswas, S.K. et al. (2006) A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Blood. 107: 2112-22.
  9. Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
    Am J Pathol. 173: 119-29.
  10. Gonzalez-Juarrero, M. et al. (2013) Polar Lipids of Burkholderia pseudomallei Induce Different Host Immune Responses.
    PLoS One. 8: e80368.