IL-10 antibody | JES5-2A5
IL-10 has a wide range of biological properties, including the inhibition of macrophage and T helper cell mediated cytokine synthesis, and the suppression of the delayed hypersensitivity response.
Rat anti Mouse Interleukin-10 antibody, clone JES5-2A5 is reported to neutralize the activity of murine IL-10 (Weber-Nordt et al. 1994).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Goat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Recombinant Mouse IL-10.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized rats were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- GO:0002875 negative regulation of chronic inflammatory response to antigenic stimulus
- GO:0042742 defense response to bacterium
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- GO:0030886 negative regulation of myeloid dendritic cell activation
- GO:0032689 negative regulation of interferon-gamma production
- GO:0032695 negative regulation of interleukin-12 production
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0044130 negative regulation of growth of symbiont in host
- GO:0043193 positive regulation of gene-specific transcription
- GO:0045348 positive regulation of MHC class II biosynthetic process
- GO:0060670 branching involved in embryonic placenta morphogenesis
- For research purposes only
Applications of IL-10 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/10||1/100|
|Immunohistology - Frozen|
- 1 Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Secondary Antibodies Available
|Description||Product Code||Applications||Pack Size||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||C E P WB||1 ml|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||C E IF P WB||0.5 mg|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||F IF WB||0.1 mg|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||F||1 mg|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||C E P RE||1 mg|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||F||0.5 ml|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||F IF WB||0.1 mg|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||C E P||0.5 mg|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||F||0.5 ml|
Product Specific References
References for IL-10 antibody
Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunological Review 127: 5-24.
Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
J Immunol Methods. 166 (2): 201-14.
Nishigori, C. et al. (1998) HindIII liposomes suppress delayed-type hypersensitivity responses in vivo and induce epidermal IL-10 in vitro.
J Immunol. 161 (6): 2684-91.
Weber-nordt, R.M. et al. (1994) Lipopolysaccharide-dependent induction of IL-10 receptor expression on murine fibroblasts.
J Immunol. 153 (8): 3734-44.
Rennick, D. et al. (1995) Cofactors are essential for stem cell factor-dependent growth and maturation of mast cell progenitors: comparative effects of interleukin-3 (IL-3), IL-4, IL-10, and fibroblasts.
Blood. 85: 57-65.
Biswas, S.K. et al. (2006) A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
Blood. 107: 2112-22.
Gaupp, S. et al. (2008) Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines.
Am J Pathol. 173: 119-29.
Gonzalez-Juarrero, M. et al. (2013) Polar Lipids of Burkholderia pseudomallei Induce Different Host Immune Responses.
PLoS One. 8: e80368.
Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
Vet Immunol Immunopathol. 202: 74-84.
Hammond, J.W. et al. (2018) HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q.
Glia. 66 (12): 2563-74.
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