IgM antibody | LO-MM-9
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Rat anti Mouse IgM Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MM-9
- Isotype
- IgG2a
- Specificity
- IgM
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgM, clone LO-MM-9 is directed against the mu chain of mouse IgM. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Polyclonal and monoclonal mouse IgM.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml (as a coating antibody) | |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgM antibody
-
Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
Eur J Immunol. 4 (1): 44-8. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71: 9-16. -
Busund, L.T. et al. (2003) Spontaneously formed tumorigenic hybrids of Meth A sarcoma cells and macrophages in vivo.
Int J Cancer. 106 (2): 153-9. -
Achtman AH et al. (2003) Plasmodium chabaudi chabaudi infection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution.
J Immunol. 171 (1): 317-24. -
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71. -
Bermejo, D.A. et al. (2011) Trypanosoma cruzi infection induces a massive extrafollicular and follicular splenic B-cell response which is a high source of non-parasite-specific antibodies.
Immunology. 132 (1): 123-33. -
Sreekanthreddy, P. et al. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions.
F1000Res. 4: 1279.
- RRID
- AB_321869
- UniProt
- P01872
- P01873
- Entrez Gene
- Ighm
- Ighm
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0003823 antigen binding
- GO:0004888 transmembrane receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0019882 antigen processing and presentation
- GO:0030890 positive regulation of B cell proliferation
- View More GO Terms
- GO:0042571 immunoglobulin complex, circulating
- GO:0043410 positive regulation of MAPKKK cascade
- GO:0045022 early endosome to late endosome transport
- GO:0045807 positive regulation of endocytosis
- GO:0048471 perinuclear region of cytoplasm
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050853 B cell receptor signaling pathway
- GO:0016021 integral to membrane
MCA199
154654
161102
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