IgM antibody | LO-MM-9

Rat anti Mouse IgM Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MM-9
Isotype
IgG2a
Specificity
IgM
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA199
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E 0.25 mg loader
List Price Your Price
loader
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Rat anti Mouse IgM, clone LO-MM-9 is directed against the mu chain of mouse IgM.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on mouse IgM/Sepharose 4B from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Polyclonal and monoclonal mouse IgM.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the IR983F rat myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml (as a coating antibody)
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for IgM antibody

  1. Achtman AH et al. (2003) Plasmodium chabaudi chabaudi infection in mice induces strong B cell responses and striking but temporary changes in splenic cell distribution.
    J Immunol. 171 (1): 317-24.
  2. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J Immunol Methods. 71: 9-16.
  3. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  4. Bermejo, D.A. et al. (2011) Trypanosoma cruzi infection induces a massive extrafollicular and follicular splenic B-cell response which is a high source of non-parasite-specific antibodies.
    Immunology. 132 (1): 123-33.
  5. Busund, L.T. et al. (2003) Spontaneously formed tumorigenic hybrids of Meth A sarcoma cells and macrophages in vivo.
    Int J Cancer. 106 (2): 153-9.
  6. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  7. Sreekanthreddy, P. et al. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions.
    F1000Res. 4: 1279.

RRID
AB_321869
UniProt
P01872
P01873
Entrez Gene
Ighm
Ighm
GO Terms
GO:0000187 activation of MAPK activity
GO:0005515 protein binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0003823 antigen binding
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0042571 immunoglobulin complex, circulating
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway
GO:0016021 integral to membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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