IgG3 antibody | LO-MG3-13

Rat anti Mouse IgG3

Product Type
Monoclonal Antibody
Clone
LO-MG3-13
Isotype
IgG1
Specificity
IgG3
Region
HEAVY CHAIN

Product Code Applications Pack Size List Price Your Price Qty
MCA1292
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse IgG3 antibody, clone LO-MG3-13 recognizes murine IgG3 (gamma heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on mouse IgG3/Sepharose 4B from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Immunogen
Polyclonal mouse IgG
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml (coating)
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This antibody may be used as a coating antibody in a sandwich ELISA in combination with LO-MG3-7 (MCA423B) and Streptavidin:HRP (STAR5B) as the detection reagents.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
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loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
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loader
Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
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loader
Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

References for IgG3 antibody

  1. Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
    Int Immunol. 5 (1): 71-8.
  2. Savignac, M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185 (12): 7527-36.
  3. Banerjee, K. et al. (2009) Enzymatic removal of mannose moieties can increase the immune response to HIV-1 gp120 in vivo.
    Virology. 389 (1-2): 108-21.
  4. Banerjee, K. et al. (2012) Occluding the mannose moieties on human immunodeficiency virus type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.
    AIDS Res Hum Retroviruses. 28 (2): 206-14.

RRID
AB_321848
UniProt
P03987
Entrez Gene
AI324046
GO Terms
GO:0005886 plasma membrane
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005576 extracellular region
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products