IgG3 antibody | LO-MG3-13
Rat anti Mouse IgG3 antibody, clone LO-MG3-13 recognizes murine IgG3 (gamma heavy chain) and does not cross react with other murine immunoglobulin classes or subclasses.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on mouse IgG3/Sepharose 4B from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Polyclonal mouse IgG
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized Lou/c rats were fused with cells of the rat IR983F myeloma cell line.
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P03987 Related reagents
- Entrez Gene
- AI324046 Related reagents
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005576 extracellular region
- For research purposes only
Applications of IgG3 antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)||STAR131A||1 ml||C E P WB|
|Goat anti Rat IgG:Biotin (Mouse Adsorbed)||STAR131B||0.5 mg||C E IF P WB|
|Rabbit F(ab')2 anti Rat IgG:Dylight®800||STAR16D800GA||0.1 mg||F IF WB|
|Rabbit F(ab')2 anti Rat IgG:FITC||STAR17B||1 mg||F|
|Rabbit F(ab')2 anti Rat IgG:HRP||STAR21B||1 mg||C E P RE|
|Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)||STAR69||0.5 ml||F|
|Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)||STAR71D549GA||0.1 mg||F IF|
|Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)||STAR71D649GA||0.1 mg||F IF|
|Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)||STAR71D800GA||0.1 mg||F IF WB|
|Goat anti Rat IgG:HRP (Mouse Adsorbed)||STAR72||0.5 mg||C E P|
|Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)||STAR73||0.5 ml||F|
Product Specific References
References for IgG3 antibody
Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
Int Immunol. 5 (1): 71-8.
Savignac, M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185 (12): 7527-36.
Banerjee, K. et al. (2009) Enzymatic removal of mannose moieties can increase the immune response to HIV-1 gp120 in vivo.
Virology. 389 (1-2): 108-21.
Banerjee, K. et al. (2012) Occluding the mannose moieties on human immunodeficiency virus type 1 gp120 with griffithsin improves the antibody responses to both proteins in mice.
AIDS Res Hum Retroviruses. 28 (2): 206-14.