IgG2a antibody | LO-MG2a-7
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|Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7 recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice).|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide
- Purified IgG from BALB/c mice.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|ELISA||5ug/ml as coating antibody|
References for IgG2a antibody
Jarman, E.R. & Lamb, J.R. (2004) Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling.
Immunology. 112 (4): 631-42.
Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
Prot Biol Fluids 615 - 8. Pergamon Press, Oxford and New York.
Nagao, K. et al. (2003) Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.
Am J Respir Cell Mol Biol. 29: 314-20.
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9.
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16.
Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
Phil Sci Lett 2: 38- 48.
Ormstad, H. et al. (2003) The effect of endotoxin on the production of IgE, IgG1 and IgG2a antibodies against the cat allergen Fel d 1 in mice
Toxicology. 188: 309-18.
Ormstad, H. et al. (2000) The fungal cell wall component beta-1,3-glucan has an adjuvant effect on the allergic response to ovalbumin in mice.
J Toxicol Environ Health A. 61: 55-67.
View The Latest Product References
Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
Vaccine. 21: 549-61.
Tan, L.K. et al. (2006) Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production.
Vaccine. 24: 5762-71.
Instanes, C. and Hetland, G. (2004) Deoxynivalenol (DON) is toxic to human colonic, lung and monocytic cell lines, but does not increase the IgE response in a mouse model for allergy.
Toxicology. 204: 13-21.
Liedén, A. et al. (2009) Cornulin, a marker of late epidermal differentiation, is down-regulated in eczema.
Wolfowicz, C.B. et al. (2003) Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization.
Vaccine. 21: 1195-204.
Hayes, K.S. et al. (2017) Chronic Trichuris muris infection causes neoplastic change in the intestine and exacerbates tumour formation in APC min/+ mice.
PLoS Negl Trop Dis. 11 (6): e0005708.
DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 11 (4) Apr 11 [Epub ahead of print].
Steel, N. et al. (2019) TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog. 15 (4): e1007657.
Querinjean, P. et al. (1972) Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats.
Eur J Biochem. 31 (2): 354-9.
- Entrez Gene
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
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