IgG2a antibody | LO-MG2a-7
Rat anti Mouse IgG2a antibody, clone LO-MG2a-9 (MCA421P) used to evaluate pathogen specific IgG2a production in mice following Trichuris muris infection by ELISA.
Image caption:
E IgG2a production in naïve and T. muris infected APCmin/+ mice serum at day 42 p.i. * p<0.05, *** p<0.0005 n = 4 per group.
From: Hayes KS, Cliffe LJ, Bancroft AJ, Forman SP, Thompson S, Booth C, et al. (2017)
Chronic Trichuris muris infection causes neoplastic change in the intestine and exacerbates tumour formation in APC min/+ mice.
PLoS Negl Trop Dis 11(6): e0005708.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Biotin labeled Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 (MCA421B) used for antibody class evaluation by ELISA.
Image caption:
Anti-IGF1R and anti-HER2/neu antibodies elicited by cell vaccines co-targeting HER2/neu and IGF1R.
Analysis of antibody isotypes induced by cell vaccines as in Figure 3A (n = 3 mice per group).
From: De Giovanni C, Landuzzi L, Palladini A, Ianzano ML, Nicoletti G, Ruzzi F, Amici A, Croci S, Nanni P, Lollini PL.
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 2019 Apr 11;11(4). pii: E517.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Biotinylated Rat anti Mouse IgG2a antibody, clone LO-MG2a-7 (MCA421B) used to assess parasite specific serum IgG2a levels in mice by ELISA.
Image caption:
Successful depletion of Foxp3+ Tregs during T. spiralis infection results in no parasite-specific antibody or mastocytosis differences, while adoptive transfer of Tregs restores the small intestinal lamina propria population in Itgb8 (CD11c-cre) mice.
DEREG mice were treated every 2 days with 200 ng diphtheria toxin or PBS (Control) 2 days prior to infection with 300 T. spiralis larvae and examined at the indicated time-points post-infection. Parasite-specific serum IgG1 and IgG2a levels in Control and DEREG mice at day 15 post-infection, obtained via ELISA.
From: Steel N, Faniyi AA, Rahman S, Swietlik S, Czajkowska BI, Chan BT, et al. (2019)
TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog 15(4): e1007657.
doi: 10.1371/journal.ppat.1007657
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse IgG2a Heavy Chain:Biotin
Rat anti Mouse IgG2a Heavy Chain:FITC
Rat anti Mouse IgG2a Heavy Chain:HRP
Rat anti Mouse IgG2a Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG2a-7
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA421B | E | 0.5 mg |
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| MCA421F | F | 0.5 mg |
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| MCA421P | E | 0.5 mg |
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| MCA421 | E | 0.25 mg |
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Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7 recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice).
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.01% Thiomersal 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified IgG from BALB/c mice.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01863
- P01865
- P01864
- Entrez Gene
- Igh-1a
- Igh-1a
- Igh-1a
- GO Terms
- GO:0003823 antigen binding
- GO:0002455 humoral immune response mediated by circulating immunoglobulin
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- Regulatory
- For research purposes only
Applications of IgG2a antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.5ug/ml | 1.0ug/ml | |
| Flow Cytometry | 2.5 ug/ml | ||
| ELISA | 0.5ug/ml | ||
| ELISA | 5ug/ml as coating antibody |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using apprpriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
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| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
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| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
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| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
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| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
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| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
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| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
|
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| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
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| AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
| TMB Signal+ | BUF054A | E | 100 ml | ||
| TMB Core | BUF056A | E | 100 ml | ||
| TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG2a antibody
-
Jarman, E.R. & Lamb, J.R. (2004) Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling.
Immunology. 112 (4): 631-42. -
Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
Prot Biol Fluids 615 - 8. Pergamon Press, Oxford and New York. -
Nagao, K. et al. (2003) Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.
Am J Respir Cell Mol Biol. 29: 314-20. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
J Immunol Methods. 66 (2): 261-9. -
Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
J Immunol Methods. 71 (1): 9-16. -
Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
-
Ormstad, H. et al. (2003) The effect of endotoxin on the production of IgE, IgG1 and IgG2a antibodies against the cat allergen Fel d 1 in mice
Toxicology. 188: 309-18. -
Ormstad, H. et al. (2000) The fungal cell wall component beta-1,3-glucan has an adjuvant effect on the allergic response to ovalbumin in mice.
J Toxicol Environ Health A. 61: 55-67. -
Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
Vaccine. 21: 549-61. -
Tan, L.K. et al. (2006) Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production.
Vaccine. 24: 5762-71. -
Instanes, C. and Hetland, G. (2004) Deoxynivalenol (DON) is toxic to human colonic, lung and monocytic cell lines, but does not increase the IgE response in a mouse model for allergy.
Toxicology. 204: 13-21. -
Liedén, A. et al. (2009) Cornulin, a marker of late epidermal differentiation, is down-regulated in eczema.
Allergy. 64:304-11. -
Wolfowicz, C.B. et al. (2003) Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization.
Vaccine. 21: 1195-204. -
Hayes, K.S. et al. (2017) Chronic Trichuris muris infection causes neoplastic change in the intestine and exacerbates tumour formation in APC min/+ mice.
PLoS Negl Trop Dis. 11 (6): e0005708. -
DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 11 (4) Apr 11 [Epub ahead of print]. -
Steel, N. et al. (2019) TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog. 15 (4): e1007657.
Further Reading
-
Querinjean, P. et al. (1972) Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats.
Eur J Biochem. 31 (2): 354-9.
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