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IgG2a antibody | LO-MG2a-7

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Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 thumbnail image 1 Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 thumbnail image 2 Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 thumbnail image 3
Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 gallery image 1
Published customer image:
Rat anti Mouse IgG2a antibody, clone LO-MG2a-9 (MCA421P) used to evaluate pathogen specific IgG2a production in mice following Trichuris muris infection by ELISA.
Image caption:
E IgG2a production in naïve and T. muris infected APCmin/+ mice serum at day 42 p.i. * p<0.05, *** p<0.0005 n = 4 per group.

From: Hayes KS, Cliffe LJ, Bancroft AJ, Forman SP, Thompson S, Booth C, et al. (2017)
Chronic Trichuris muris infection causes neoplastic change in the intestine and exacerbates tumour formation in APC min/+ mice.
PLoS Negl Trop Dis 11(6): e0005708.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 gallery image 2
Published customer image:
Biotin labeled Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 (MCA421B) used for antibody class evaluation by ELISA.
Image caption:
Anti-IGF1R and anti-HER2/neu antibodies elicited by cell vaccines co-targeting HER2/neu and IGF1R.
Analysis of antibody isotypes induced by cell vaccines as in Figure 3A (n = 3 mice per group).

From: De Giovanni C, Landuzzi L, Palladini A, Ianzano ML, Nicoletti G, Ruzzi F, Amici A, Croci S, Nanni P, Lollini PL.
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 2019 Apr 11;11(4). pii: E517.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Mouse IgG2a Heavy Chain Antibody, clone LO-MG2a-7 gallery image 3
Published customer image:
Biotinylated Rat anti Mouse IgG2a antibody, clone LO-MG2a-7 (MCA421B) used to assess parasite specific serum IgG2a levels in mice by ELISA.
Image caption:
Successful depletion of Foxp3+ Tregs during T. spiralis infection results in no parasite-specific antibody or mastocytosis differences, while adoptive transfer of Tregs restores the small intestinal lamina propria population in Itgb8 (CD11c-cre) mice.
DEREG mice were treated every 2 days with 200 ng diphtheria toxin or PBS (Control) 2 days prior to infection with 300 T. spiralis larvae and examined at the indicated time-points post-infection. Parasite-specific serum IgG1 and IgG2a levels in Control and DEREG mice at day 15 post-infection, obtained via ELISA.

From: Steel N, Faniyi AA, Rahman S, Swietlik S, Czajkowska BI, Chan BT, et al. (2019)
TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog 15(4): e1007657.
doi: 10.1371/journal.ppat.1007657
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Mouse IgG2a Heavy Chain:Biotin

Rat anti Mouse IgG2a Heavy Chain:FITC

Rat anti Mouse IgG2a Heavy Chain:HRP

Rat anti Mouse IgG2a Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG2a-7
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA421B
Datasheet
E
SDS
0.5 mg loader

MCA421F
Datasheet
F
SDS
0.5 mg loader

MCA421P
Datasheet
E
SDS
0.5 mg loader

MCA421
Datasheet
E
SDS
0.25 mg loader

Rat anti Mouse IgG2a:Biotin, clone LO-MG2a-7 recognizes the gamma 2a heavy chain of mouse immunoglobulin and does not cross-react with other murine immunoglobulin classes or subclasses. Rat anti Mouse IgG2a Heavy Chain antibody, clone LO-MG2a-7 recognizes an allotypic determinant upon mouse IgG2a. It recognizes the IgHIa allotype (as expressed in Balb/c mice), but not the IgHIb allotype (as expressed in C57/BL mice).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified IgG from BALB/c mice.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P01863
P01865
P01864
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgG2a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml 1.0ug/ml
Flow Cytometry 2.5 ug/ml
ELISA 0.5ug/ml
ELISA 5ug/ml as coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using apprpriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml

Product Specific References

References for IgG2a antibody

  1. Jarman, E.R. & Lamb, J.R. (2004) Reversal of established CD4+ type 2 T helper-mediated allergic airway inflammation and eosinophilia by therapeutic treatment with DNA vaccines limits progression towards chronic inflammation and remodelling.
    Immunology. 112 (4): 631-42.
  2. Bazin, H. (1982) Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line.
    Prot Biol Fluids 615 - 8. Pergamon Press, Oxford and New York.
  3. Nagao, K. et al. (2003) Role of prostaglandin I2 in airway remodeling induced by repeated allergen challenge in mice.
    Am J Respir Cell Mol Biol. 29: 314-20.
  4. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants.
    J Immunol Methods. 66 (2): 261-9.
  5. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum.
    J Immunol Methods. 71 (1): 9-16.
  6. Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
    Phil Sci Lett 2: 38- 48.
  7. Ormstad, H. et al. (2003) The effect of endotoxin on the production of IgE, IgG1 and IgG2a antibodies against the cat allergen Fel d 1 in mice
    Toxicology. 188: 309-18.
  8. Ormstad, H. et al. (2000) The fungal cell wall component beta-1,3-glucan has an adjuvant effect on the allergic response to ovalbumin in mice.
    J Toxicol Environ Health A. 61: 55-67.
  9. Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
    Vaccine. 21: 549-61.
  10. Tan, L.K. et al. (2006) Intramuscular immunization with DNA construct containing Der p 2 and signal peptide sequences primed strong IgE production.
    Vaccine. 24: 5762-71.
  11. Instanes, C. and Hetland, G. (2004) Deoxynivalenol (DON) is toxic to human colonic, lung and monocytic cell lines, but does not increase the IgE response in a mouse model for allergy.
    Toxicology. 204: 13-21.
  12. Liedén, A. et al. (2009) Cornulin, a marker of late epidermal differentiation, is down-regulated in eczema.
    Allergy. 64:304-11.
  13. Wolfowicz, C.B. et al. (2003) Expression and immunogenicity of the major house dust mite allergen Der p 1 following DNA immunization.
    Vaccine. 21: 1195-204.
  14. Hayes, K.S. et al. (2017) Chronic Trichuris muris infection causes neoplastic change in the intestine and exacerbates tumour formation in APC min/+ mice.
    PLoS Negl Trop Dis. 11 (6): e0005708.
  15. DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
    Cancers (Basel). 11 (4) Apr 11 [Epub ahead of print].
  16. Steel, N. et al. (2019) TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
    PLoS Pathog. 15 (4): e1007657.

Further Reading

  1. Querinjean, P. et al. (1972) Transplantable immunoglobulin-secreting tumours in rats. Purification and chemical characterization of four kappa chains from LOU-Wsl rats.
    Eur J Biochem. 31 (2): 354-9.

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