IgG1 antibody | LO-MG1-2
Rat anti Mouse IgG1 heavy chain antibody, clone LO-MG1-2 used as a detection reagent for mouse IgG1 by ELISA
Image caption:
Multi-epitope peptide-specific antibody production. BALB/c mice (n = 9/group) immunized either with individual peptide emulsified in CFA/IFA or PBS alone, were bled 15 days post third immunization and sera were separated. (A,B) total IgG Abs, and (C) IgG1 and IgG2a Abs against each peptide were assessed by ELISA. The results are expressed as OD450 ± SD. Significant differences between groups of mice immunized with each synthetic peptide emulsified in CFA/IFA and the group of mice immunized with CFA/IFA alone are indicated by * (P < 0.05).
From: Agallou M, Athanasiou E, Koutsoni O, Dotsika E, Karagouni E. Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins. Front Immunol. 2014 Jun 10;5:268.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Biotin labeled Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 (MCA336B) used for antibody class evaluation by ELISA.
Image caption:
Anti-IGF1R and anti-HER2/neu antibodies elicited by cell vaccines co-targeting HER2/neu and IGF1R.
Analysis of antibody isotypes induced by cell vaccines as in Figure 3A (n = 3 mice per group).
From: De Giovanni C, Landuzzi L, Palladini A, Ianzano ML, Nicoletti G, Ruzzi F, Amici A, Croci S, Nanni P, Lollini PL.
Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 2019 Apr 11;11(4). pii: E517.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Biotinylated Rat anti Mouse IgG1 antibody, clone LO-MG1-2 (MCA336B) used to assess parasite specific serum IgG1 levels in mice by ELISA.
Image caption:
Successful depletion of Foxp3+ Tregs during T. spiralis infection results in no parasite-specific antibody or mastocytosis differences, while adoptive transfer of Tregs restores the small intestinal lamina propria population in Itgb8 (CD11c-cre) mice.
DEREG mice were treated every 2 days with 200 ng diphtheria toxin or PBS (Control) 2 days prior to infection with 300 T. spiralis larvae and examined at the indicated time-points post-infection. Parasite-specific serum IgG1 and IgG2a levels in Control and DEREG mice at day 15 post-infection, obtained via ELISA.
From: Steel N, Faniyi AA, Rahman S, Swietlik S, Czajkowska BI, Chan BT, et al. (2019)
TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog 15(4): e1007657.
doi: 10.1371/journal.ppat.1007657
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse IgG1 Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-MG1-2
- Isotype
- IgG1
- Specificity
- IgG1
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified mouse IgG1 from BALB/c mice
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as coating antibody |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgG1 antibody
-
Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
Int Immunol. 5 (1): 71-8. -
Song, J. et al. (2000) Heterogeneous distribution of isoactins in cultured vascular smooth muscle cells does not reflect segregation of contractile and cytoskeletal domains.
J Histochem Cytochem. 48 (11): 1441-52. -
Blackwell, N.M. & Else, K.J. (2002) A comparison of local and peripheral parasite-specific antibody production in different strains of mice infected with Trichuris muris.
Parasite Immunol. 24 (4): 203-11. -
Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
Vaccine. 21 (5-6): 549-61. -
Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
Vaccine. 24: 4102-10. -
Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
-
Nakanishi, S. et al. (2010) Sequence analysis of a bacteriocinogenic plasmid of Clostridium butyricum and expression of the bacteriocin gene in Escherichia coli.
Anaerobe. 16: 253-7. -
Hjerpe, C. et al. (2010) Dendritic cells pulsed with malondialdehyde modified low density lipoprotein aggravate atherosclerosis in Apoe(-/-) mice.
Atherosclerosis. 209 (2): 436-41.
View The Latest Product References
-
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71. -
Agallou, M. et al. (2014) Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins.
Front Immunol. 5: 268. -
Kato, G. et al. (2014) β2 adrenergic agonist attenuates house dust mite-induced allergic airway inflammation through dendritic cells.
BMC Immunol. 15: 39. -
Doerfler, P.A. et al. (2015) BAFF Blockade Prevents Anti-Drug Antibody Formation in a Mouse Model of Pompe Disease.
Clin Immunol. pii: S1521-6616(15)00125-4. -
Kretschmer, B. et al. (2015) Anti-CD83 promotes IgG1 isotype switch in marginal zone B cells in response to TI-2 antigen.
Immunobiology. 220 (8): 964-75. -
Doerfler, P.A. et al. (2016) Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease.
Hum Gene Ther. 27 (1): 43-59. -
Margaroni, M. et al. (2017) Vaccination with poly(D,L-lactide-co-glycolide) nanoparticles loaded with soluble Leishmania antigens and modified with a TNFα-mimicking peptide or monophosphoryl lipid A confers protection against experimental visceral leishmaniasis.
Int J Nanomedicine. 12: 6169-84. -
Steel, N. et al. (2019) TGFβ-activation by dendritic cells drives Th17 induction and intestinal contractility and augments the expulsion of the parasite Trichinella spiralis. in mice.
PLoS Pathog. 15 (4): e1007657. -
DeGiovanni, C. et al. (2019) Cancer Vaccines Co-Targeting HER2/Neu and IGF1R.
Cancers (Basel). 11 (4) Apr 11 [Epub ahead of print]. -
Scaramozza, A. et al. (2019) Lineage Tracing Reveals a Subset of Reserve Muscle Stem Cells Capable of Clonal Expansion under Stress.
Cell Stem Cell. 24 (6): 944-957.e5. -
Salem, E. et al. (2019) Pathogenesis, Host Innate Immune Response, and Aerosol Transmission of Influenza D Virus in Cattle.
J Virol.93(7):e01853-18. -
Doshi, B.S. et al. (2021) B cell-activating factor modulates the factor VIII immune response in hemophilia A.
J Clin Invest. 131(8):142906. -
Petta, I. et al. (2024) Myeloid A20 is critical for alternative macrophage polarization and type-2 immune mediated helminth resistance
Front Immunol. 15: 21 Mar [Epub ahead of print]
- RRID
- AB_321954
- UniProt
- P01869
- P01868
- Entrez Gene
- Ighg1
- Ighg1
- GO Terms
- GO:0001788 antibody-dependent cellular cytotoxicity
- GO:0001798 positive regulation of type IIa hypersensitivity
- GO:0001812 positive regulation of type I hypersensitivity
- GO:0003823 antigen binding
- GO:0006910 phagocytosis, recognition
- GO:0006911 phagocytosis, engulfment
- GO:0006958 complement activation, classical pathway
- GO:0019731 antibacterial humoral response
- GO:0042571 immunoglobulin complex, circulating
- View More GO Terms
- GO:0050766 positive regulation of phagocytosis
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
MCA336
152980
156864
161146
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