IgG1 antibody | LO-MG1-2

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Rat anti Mouse IgG1 Heavy Chain:Biotin

Rat anti Mouse IgG1 Heavy Chain:FITC

Rat anti Mouse IgG1 Heavy Chain:HRP

Rat anti Mouse IgG1 Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-MG1-2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA336B E 0.5 mg
MCA336F F 0.5 mg
MCA336P E 0.5 mg
MCA336 E 0.25 mg
Rat anti Mouse IgG1 Heavy Chain antibody, clone LO-MG1-2 recognizes murine IgG1, and does not bind other mouse immunoglobulin classes or subclasses.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide
50%Glycerol
Preservative Stabilisers
0.09%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified mouse IgG1 from BALB/c mice
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01869 Related reagents
P01868 Related reagents
Entrez Gene
Ighg1 Related reagents
Ighg1 Related reagents
GO Terms
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0003823 antigen binding
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0050766 positive regulation of phagocytosis
GO:0005886 plasma membrane
GO:0016021 integral to membrane
Regulatory
For research purposes only

Applications of IgG1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5 ug/ml
Flow Cytometry 5ug/ml
ELISA 500ng/ml
ELISA 5ug/ml as coating antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropritae negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using approriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
ELISA
This product may be used in a direct ELISA or as a detection reagent in a sandwich ELISA together with MCA1289 as the capture reagent.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:FITC MCA1211F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgG1 antibody

  1. Song, J. et al. (2000) Heterogeneous distribution of isoactins in cultured vascular smooth muscle cells does not reflect segregation of contractile and cytoskeletal domains.
    J Histochem Cytochem. 48 (11): 1441-52.
  2. Denis, O. et al. (1993) Resting B cells can act as antigen presenting cells in vivo and induce antibody responses.
    Int Immunol. 5 (1): 71-8.
  3. Nakanishi, S. et al. (2010) Sequence analysis of a bacteriocinogenic plasmid of Clostridium butyricum and expression of the bacteriocin gene in Escherichia coli.

    Anaerobe. 16: 253-7.
  4. Echeverria, P.C. et al. (2006) Potent antigen-specific immunity to Toxoplasma gondii in adjuvant-free vaccination system using Rop2-Leishmania infantum Hsp83 fusion protein.
    Vaccine. 24: 4102-10.
  5. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  6. Agallou, M. et al. (2014) Experimental Validation of Multi-Epitope Peptides Including Promising MHC Class I- and II-Restricted Epitopes of Four Known Leishmania infantum Proteins.
    Front Immunol. 5: 268.
  7. Doerfler, P.A. et al. (2015) BAFF Blockade Prevents Anti-Drug Antibody Formation in a Mouse Model of Pompe Disease.
    Clin Immunol. pii: S1521-6616(15)00125-4.
  8. Ramos, J.D.A. et al. (2009) Characterization of Blo t 11 Monoclonal Antibodies with Constant Region Mutations
    Phil Sci Lett. 2(1): 38-48.
  9. Blackwell, N.M. & Else, K.J. (2002) A comparison of local and peripheral parasite-specific antibody production in different strains of mice infected with Trichuris muris.
    Parasite Immunol. 24 (4): 203-11.
  10. Hall, G. et al. (2003) Suppression of allergen reactive Th2 mediated responses and pulmonary eosinophilia by intranasal administration of an immunodominant peptide is linked to IL-10 production.
    Vaccine. 21 (5-6): 549-61.
  11. Hjerpe, C. et al. (2010) Dendritic cells pulsed with malondialdehyde modified low density lipoprotein aggravate atherosclerosis in Apoe(-/-) mice.
    Atherosclerosis. 209 (2): 436-41.
  12. Kretschmer, B. et al. (2015) Anti-CD83 promotes IgG1 isotype switch in marginal zone B cells in response to TI-2 antigen.
    Immunobiology. 220 (8): 964-75.
  13. Doerfler, P.A. et al. (2016) Copackaged AAV9 Vectors Promote Simultaneous Immune Tolerance and Phenotypic Correction of Pompe Disease.
    Hum Gene Ther. 27 (1): 43-59.
  14. Kato, G. et al. (2014) β2 adrenergic agonist attenuates house dust mite-induced allergic airway inflammation through dendritic cells.
    BMC Immunol. 15: 39.
  15. Margaroni, M. et al. (2017) Vaccination with poly(D,L-lactide-co-glycolide) nanoparticles loaded with soluble Leishmania antigens and modified with a TNFα-mimicking peptide or monophosphoryl lipid A confers protection against experimental visceral leishmaniasis.
    Int J Nanomedicine. 12: 6169-84.