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IgE antibody | LO-ME-3

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Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 thumbnail image 1 Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 thumbnail image 2 Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 thumbnail image 3
Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 gallery image 1
Published customer image:
Rat anti Mouse IgE heavy chain antibody, clone LO-ME-3 (MCA419) used for the detection of mouse IgE by ELISA
Image caption:
Asthma model. Cerk-/- and WT mice were exposed to OVA-aerosol as described in the Experimental section. BAL fluid and blood were collected at 48 hours after OVA challenge. The number of infiltrated inflammatory cells (A), OVA-specific IgG1 and IgE in serum (B) and PGE2 in BAL fluid (C) are represented as the mean ± SEM of 8 animals per group.

From: Niwa S, Urtz N, Baumruker T, Billich A, Bornancin F.
Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
Lipids Health Dis. 2010 Jan 6;9:1.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 gallery image 2
Published customer image:
Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 (MCA419) used as a capture reagent in an ELISA for the measurement of total and ovalbumin specific IgE in murine plasma samples.
Image caption:
Effects of SSTW on total and OVA-specific IgE levels in plasma. Data were obtained using ELISA analysis. a Total IgE level and (b) OVA-specific IgE level. NC, normal control group (PBS only); OVA, OVA-induced group; Mon, montelukast (30 mg/kg) + OVA-induced group; SSTW-100, SSTW (100 mg/kg) + OVA-induced group; SSTW-200, SSTW (200 mg/kg) + OVA-induced group. Each bar represents the mean ± SEM (n = 6 per group). Significant differences at ## P < 0.01 compared with the NC group. Significant differences at ** P < 0.01 compared with the OVA-induced group

From: Jeon WY, Shin HK, Shin IS, Kim SK, Lee MY.
Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 2015 Sep 18;15(1):329.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Anti Mouse IgE Heavy Chain Antibody, clone LO-ME-3 gallery image 3
Published customer image:
HRP conjugated Rat anti Mouse IgE antibody, clone LO-ME-3 (MCA419P) used for the evaluation of IgE levels in mouse serum samples by ELISA.
Image caption:
IgE responses to the S. mansoni antigens RP26, sm31, serpin, SEA and SWAP during S. mansoni infection and after the treatment.
Five mice were infected with 50 S. mansoni cercariae, and the sera were collected from five mice at each time point. A group of mice was orally treated twice with 500 mg/kg PZQ at 8 weeks post-infection. The experiment was repeated for 3 times. The means of the IgE level to the S. mansoni recombinant RP26 (A), sm31 (B), serpin (C), and crude SWAP (D) and SEA (E) are shown with the standard errors. The dashed line represents the cut-off value (mean + 3SD of the OD of the pre-infected group). IgE levels of the treated group (open diamonds) were compared with those of the untreated group (closed circles) and with those of pre-infection samples taken at week 0 (closed triangles). **p <0.01 and ***p <0.001.

From: Mohammed ES, Nakamura R, Kalenda YD, et al.
Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.
PLoS Negl Trop Dis. 2020;14(9):e0008518.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rat anti Mouse IgE Heavy Chain:Biotin

Rat anti Mouse IgE Heavy Chain:FITC

Rat anti Mouse IgE Heavy Chain:HRP

Rat anti Mouse IgE Heavy Chain

Product Type
Monoclonal Antibody
Clone
LO-ME-3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA419B
Datasheet
E
SDS
0.5 mg loader

MCA419F
Datasheet
F
SDS
0.5 mg loader

MCA419P
Datasheet
E
SDS
0.5 mg loader

MCA419
Datasheet
E
SDS
0.25 mg loader

Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 is = 3 x 109M-1

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Preservative Stabilisers
0.01%Thiomersal
50%Glycerol
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Purified IgE from BALB/c mice.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P06336
Entrez Gene
Gm900
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of IgE antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Western Blotting
Flow Cytometry 5ug/ml
ELISA 500ng/ml
Western Blotting
ELISA 5ug/ml as capture antibody
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml

Application Based External Images

ELISA

Product Specific References

References for IgE antibody

  1. Mojtabavi, N. et al. (2002) Long-lived Th2 memory in experimental allergic asthma.
    J Immunol. 169 (9): 4788-96.
  2. Stevens,T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust
    Toxicol.Sci. 102: 359-70.
  3. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
    J Immunol. 185:7527-36.
  4. Bemark, M. et al. (2011) A unique role of the cholera toxin A1-DD adjuvant for long-term plasma and memory B cell development.
    J Immunol. 186: 1399-410.
  5. Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
    J Immunol. 174 (1): 525-32.
  6. Komai, M. et al. (2010) A novel CC-chemokine receptor 3 antagonist, Ki19003, inhibits airway eosinophilia and subepithelial/peribronchial fibrosis induced by repeated antigen challenge in mice.
    J Pharmacol Sci. 112: 203-13.
  7. Komai, M. et al. (2003) Role of Th2 responses in the development of allergen-induced airway remodelling in a murine model of allergic asthma.
    Br J Pharmacol. 138: 912-20
  8. Lee, M.Y. et al. (2011) Protective Effects of Mentha haplocalyx Ethanol Extract (MH) in a Mouse Model of Allergic Asthma.
    Phytother Res. 25(6):863-9.
  9. Niwa, S. et al. (2010) Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
    Lipids Health Dis. 9:1.
  10. Hazebrouck, S. et al. (2009) Allergic sensitization to bovine beta-lactoglobulin: comparison between germ-free and conventional BALB/c mice.
    Int Arch Allergy Immunol. 148: 65-72.
  11. Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
    J Immunol. 187: 462-71.
  12. Kalenda, Y.D. et al. (2015) Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection.
    Parasitol Int. 64 (6): 503-12.
  13. Kim, J.H. & Ohsawa, M. (1995) Oral tolerance to ovalbumin in mice as a model for detecting modulators of the immunologic tolerance to a specific antigen.
    Biol Pharm Bull. 18 (6): 854-8.
  14. Ray, A. et al. (2015) Gut Microbial Dysbiosis Due to Helicobacter Drives an Increase in Marginal Zone B Cells in the Absence of IL-10 Signaling in Macrophages.
    J Immunol. 195 (7): 3071-85.
  15. Nakano, Y. et al. (2016) Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice.
    J Pharmacol Exp Ther. 357 (2): 258-63.
  16. Jeon, W.Y. et al. (2015) Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
    BMC Complement Altern Med. 15 (1): 329.
  17. Ziegler, T. et al. (2015) A novel regulatory macrophage induced by a helminth molecule instructs IL-10 in CD4+ T cells and protects against mucosal inflammation.
    J Immunol. 194 (4): 1555-64.
  18. Noble A & Zhao J (2016) Follicular helper T cells are responsible for IgE responses to Der p 1 following house dust mite sensitization in mice.
    Clin Exp Allergy. May 3 [Epub ahead of print]
  19. Roberts, H. et al. (2016) Characterization of neutrophil function in Papillon-Lefèvre syndrome.
    J Leukoc Biol. 100 (2): 433-44.
  20. Ikeda, M. et al. (2016) Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model of chronic asthma.
    Allergol Int. pii: S1323-8930(16)30161-7. [Epub ahead of print]
  21. González-Chomón C et al. (2016) Biomimetic contact lenses eluting olopatadine for allergic conjunctivitis.
    Acta Biomater. 41: 302-11.
  22. Maiga, M.A. et al. (2017) Neonatal mono-colonization of germ-free mice with Lactobacillus casei enhances casein immunogenicity after oral sensitization to cow's milk.
    Mol Nutr Food Res. Mar 20. [Epub ahead of print]
  23. J Wadley, A. et al. (2017) Heightened Exercise-Induced Oxidative Stress at Simulated Moderate Level Altitude vs. Sea Level in Trained Cyclists.
    Int J Sport Nutr Exerc Metab. 27 (2): 97-104.
  24. Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
    J Exp Med. May 8. pii: jem.20161807.
  25. Chesné, J. et al. (2015) Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite-induced allergic asthma model.
    J Allergy Clin Immunol. 135 (6): 1643-1643.e3.
  26. Di, C. et al. (2015) Basophil-associated OX40 ligand participates in the initiation of Th2 responses during airway inflammation.
    J Biol Chem. 290 (20): 12523-36.
  27. Mohammed, E.S. et al.. (2020) Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.
    PLoS Negl Trop Dis. 2020;14(9):e0008518.

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