IgE antibody | LO-ME-3
Rat anti Mouse IgE heavy chain antibody, clone LO-ME-3 (MCA419) used for the detection of mouse IgE by ELISA
Image caption:
Asthma model. Cerk-/- and WT mice were exposed to OVA-aerosol as described in the Experimental section. BAL fluid and blood were collected at 48 hours after OVA challenge. The number of infiltrated inflammatory cells (A), OVA-specific IgG1 and IgE in serum (B) and PGE2 in BAL fluid (C) are represented as the mean ± SEM of 8 animals per group.
From: Niwa S, Urtz N, Baumruker T, Billich A, Bornancin F.
Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
Lipids Health Dis. 2010 Jan 6;9:1.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 (MCA419) used as a capture reagent in an ELISA for the measurement of total and ovalbumin specific IgE in murine plasma samples.
Image caption:
Effects of SSTW on total and OVA-specific IgE levels in plasma. Data were obtained using ELISA analysis. a Total IgE level and (b) OVA-specific IgE level. NC, normal control group (PBS only); OVA, OVA-induced group; Mon, montelukast (30 mg/kg) + OVA-induced group; SSTW-100, SSTW (100 mg/kg) + OVA-induced group; SSTW-200, SSTW (200 mg/kg) + OVA-induced group. Each bar represents the mean ± SEM (n = 6 per group). Significant differences at ## P < 0.01 compared with the NC group. Significant differences at ** P < 0.01 compared with the OVA-induced group
From: Jeon WY, Shin HK, Shin IS, Kim SK, Lee MY.
Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 2015 Sep 18;15(1):329.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
HRP conjugated Rat anti Mouse IgE antibody, clone LO-ME-3 (MCA419P) used for the evaluation of IgE levels in mouse serum samples by ELISA.
Image caption:
IgE responses to the S. mansoni antigens RP26, sm31, serpin, SEA and SWAP during S. mansoni infection and after the treatment.
Five mice were infected with 50 S. mansoni cercariae, and the sera were collected from five mice at each time point. A group of mice was orally treated twice with 500 mg/kg PZQ at 8 weeks post-infection. The experiment was repeated for 3 times. The means of the IgE level to the S. mansoni recombinant RP26 (A), sm31 (B), serpin (C), and crude SWAP (D) and SEA (E) are shown with the standard errors. The dashed line represents the cut-off value (mean + 3SD of the OD of the pre-infected group). IgE levels of the treated group (open diamonds) were compared with those of the untreated group (closed circles) and with those of pre-infection samples taken at week 0 (closed triangles). **p <0.01 and ***p <0.001.
From: Mohammed ES, Nakamura R, Kalenda YD, et al.
Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.
PLoS Negl Trop Dis. 2020;14(9):e0008518.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
E ResetRat anti Mouse IgE Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-ME-3
- Isotype
- IgG1
- Specificity
- IgE
- Region
- HEAVY CHAIN
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 is = 3 x 109M-1 |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified IgE from BALB/c mice.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml as capture antibody | |
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for IgE antibody
-
Kim, J.H. & Ohsawa, M. (1995) Oral tolerance to ovalbumin in mice as a model for detecting modulators of the immunologic tolerance to a specific antigen.
Biol Pharm Bull. 18 (6): 854-8. -
Mojtabavi, N. et al. (2002) Long-lived Th2 memory in experimental allergic asthma.
J Immunol. 169 (9): 4788-96. -
Komai, M. et al. (2003) Role of Th2 responses in the development of allergen-induced airway remodelling in a murine model of allergic asthma.
Br J Pharmacol. 138: 912-20 -
Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
J Immunol. 174 (1): 525-32. -
Stevens,T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust
Toxicol.Sci. 102: 359-70. -
Hazebrouck, S. et al. (2009) Allergic sensitization to bovine beta-lactoglobulin: comparison between germ-free and conventional BALB/c mice.
Int Arch Allergy Immunol. 148: 65-72. -
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Komai, M. et al. (2010) A novel CC-chemokine receptor 3 antagonist, Ki19003, inhibits airway eosinophilia and subepithelial/peribronchial fibrosis induced by repeated antigen challenge in mice.
J Pharmacol Sci. 112: 203-13.
View The Latest Product References
-
Niwa, S. et al. (2010) Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
Lipids Health Dis. 9:1. -
Bemark, M. et al. (2011) A unique role of the cholera toxin A1-DD adjuvant for long-term plasma and memory B cell development.
J Immunol. 186: 1399-410. -
Lee, M.Y. et al. (2011) Protective Effects of Mentha haplocalyx Ethanol Extract (MH) in a Mouse Model of Allergic Asthma.
Phytother Res. 25(6):863-9. -
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71. -
Di, C. et al. (2015) Basophil-associated OX40 ligand participates in the initiation of Th2 responses during airway inflammation.
J Biol Chem. 290 (20): 12523-36. -
Chesné, J. et al. (2015) Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite-induced allergic asthma model.
J Allergy Clin Immunol. 135 (6): 1643-1643.e3. -
Ziegler, T. et al. (2015) A novel regulatory macrophage induced by a helminth molecule instructs IL-10 in CD4+ T cells and protects against mucosal inflammation.
J Immunol. 194 (4): 1555-64. -
Jeon, W.Y. et al. (2015) Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 15 (1): 329. -
Kalenda, Y.D. et al. (2015) Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection.
Parasitol Int. 64 (6): 503-12. -
Noble, A. & Zhao, J. (2016) Follicular helper T cells are responsible for IgE responses to Der p 1 following house dust mite sensitization in mice.
Clin Exp Allergy. 46 (8): 1075-82. -
Roberts, H. et al. (2016) Characterization of neutrophil function in Papillon-Lefèvre syndrome.
J Leukoc Biol. 100 (2): 433-44. -
González-Chomón C et al. (2016) Biomimetic contact lenses eluting olopatadine for allergic conjunctivitis.
Acta Biomater. 41: 302-11. -
Nakano, Y. et al. (2016) Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice.
J Pharmacol Exp Ther. 357 (2): 258-63. -
Ikeda, M. et al. (2017) Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model of chronic asthma.
Allergol Int. 66 (3): 432-9. -
Maiga, M.A. et al. (2017) Neonatal mono-colonization of germ-free mice with Lactobacillus casei enhances casein immunogenicity after oral sensitization to cow's milk.
Mol Nutr Food Res. 61 (9): 1600862 -
Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
J Exp Med. May 8. pii: jem.20161807. -
Yamashita, H. et al. (2017) Artificial sweeteners and mixture of food additives cause to break oral tolerance and induce food allergy in murine oral tolerance model for food allergy.
Clin Exp Allergy. 47 (9): 1204-13. -
Ghonim, M.A. et al. (2018) Sulfated non-anticoagulant heparin blocks Th2-induced asthma by modulating the IL-4/signal transducer and activator of transcription 6/Janus kinase 1 pathway.
J Transl Med. 16 (1): 243. -
Mohammed, E.S. et al.. (2020) Dynamics of serological responses to defined recombinant proteins during Schistosoma mansoni infection in mice before and after the treatment with praziquantel.
PLoS Negl Trop Dis. 2020;14(9):e0008518. -
Yamashita, H. et al. (2021) Impact of orally-administered oligosaccharides in a murine model of food allergy
Journal of Functional Foods. 85: 104643. -
Laoubi, L. et al. (2022) Epicutaneous allergen immunotherapy induces a profound and selective modulation in skin dendritic-cell subsets.
J Allergy Clin Immunol. 150 (5): 1194-208.
- RRID
- AB_321895
- UniProt
- P06336
- Entrez Gene
- Gm900
- GO Terms
- GO:0003823 antigen binding
MCA419
153478
165533
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