IgE antibody | LO-ME-3
Rat anti Mouse IgE heavy chain antibody, clone LO-ME-3 used for the detection of mouse IgE by ELISA
Image caption:
Asthma model. Cerk-/- and WT mice were exposed to OVA-aerosol as described in the Experimental section. BAL fluid and blood were collected at 48 hours after OVA challenge. The number of infiltrated inflammatory cells (A), OVA-specific IgG1 and IgE in serum (B) and PGE2 in BAL fluid (C) are represented as the mean ± SEM of 8 animals per group.
From: Niwa S, Urtz N, Baumruker T, Billich A, Bornancin F. Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice. Lipids Health Dis. 2010 Jan 6;9:1.
Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 used as a capture reagent in an ELISA for the measurement of total and ovalbumin specific IgE in murine plasma samples.
Image caption:
Effects of SSTW on total and OVA-specific IgE levels in plasma. Data were obtained using ELISA analysis. a Total IgE level and (b) OVA-specific IgE level. NC, normal control group (PBS only); OVA, OVA-induced group; Mon, montelukast (30 mg/kg) + OVA-induced group; SSTW-100, SSTW (100 mg/kg) + OVA-induced group; SSTW-200, SSTW (200 mg/kg) + OVA-induced group. Each bar represents the mean ± SEM (n = 6 per group). Significant differences at ## P < 0.01 compared with the NC group. Significant differences at ** P < 0.01 compared with the OVA-induced group
From: Jeon WY, Shin HK, Shin IS, Kim SK, Lee MY.
Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 2015 Sep 18;15(1):329.
Rat anti Mouse IgE Heavy Chain:Biotin
Rat anti Mouse IgE Heavy Chain:FITC
Rat anti Mouse IgE Heavy Chain:HRP
Rat anti Mouse IgE Heavy Chain
- Product Type
- Monoclonal Antibody
- Clone
- LO-ME-3
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| MCA419B | E | 0.5 mg | ||||
| MCA419F | F | 0.5 mg | ||||
| MCA419P | E | 0.5 mg | ||||
| MCA419 | E | 0.25 mg |
Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 recognizes the murine epsilon immunoglobulin heavy chain, and does not cross react with other murine immunoglobulin classes or subclasses. The avidity of Rat anti Mouse IgE Heavy Chain antibody, clone LO-ME-3 is = 3 x 109M-1
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide 50% Glycerol - Preservative Stabilisers
0.01% Thiomersal 50% Glycerol - Preservative Stabilisers
0.1% Sodium Azide - Immunogen
- Purified IgE from BALB/c mice.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised LOU/c rats were fused with cells of the rat IR983F myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P06336 Related reagents
- Entrez Gene
- Gm900 Related reagents
- GO Terms
- GO:0003823 antigen binding
- Regulatory
- For research purposes only
Applications of IgE antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 500ng/ml | ||
| Western Blotting | |||
| Flow Cytometry | 5ug/ml | ||
| ELISA | 500ng/ml | ||
| Western Blotting | |||
| ELISA | 5ug/ml as capture antibody | ||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | 1 ml | C E P WB | ||
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | 0.5 mg | C E IF P WB | ||
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | 0.1 mg | F IF WB | ||
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | 1 mg | F | ||
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | 1 mg | C E P RE | ||
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | 0.5 ml | F | ||
| Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) | STAR71D549GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) | STAR71D649GA | 0.1 mg | F IF | ||
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | 0.1 mg | F IF WB | ||
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | 0.5 mg | C E P | ||
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | 0.5 ml | F |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG1 Negative Control:FITC | MCA1211F | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | ||
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | ||
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgE antibody
-
Mojtabavi, N. et al. (2002) Long-lived Th2 memory in experimental allergic asthma.
J Immunol. 169 (9): 4788-96. -
Stevens,T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust
Toxicol.Sci. 102: 359-70. -
Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36. -
Bemark, M. et al. (2011) A unique role of the cholera toxin A1-DD adjuvant for long-term plasma and memory B cell development.
J Immunol. 186: 1399-410. -
Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
J Immunol. 174 (1): 525-32. -
Komai, M. et al. (2010) A novel CC-chemokine receptor 3 antagonist, Ki19003, inhibits airway eosinophilia and subepithelial/peribronchial fibrosis induced by repeated antigen challenge in mice.
J Pharmacol Sci. 112: 203-13. -
Komai, M. et al. (2003) Role of Th2 responses in the development of allergen-induced airway remodelling in a murine model of allergic asthma.
Br J Pharmacol. 138: 912-20 -
Lee, M.Y. et al. (2011) Protective Effects of Mentha haplocalyx Ethanol Extract (MH) in a Mouse Model of Allergic Asthma.
Phytother Res. 25(6):863-9. -
Niwa, S. et al. (2010) Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1-/- mice.
Lipids Health Dis. 9:1. -
Hazebrouck, S. et al. (2009) Allergic sensitization to bovine beta-lactoglobulin: comparison between germ-free and conventional BALB/c mice.
Int Arch Allergy Immunol. 148: 65-72. -
Huang, C.H. et al. (2011) Airway inflammation and IgE production induced by dust mite allergen-specific memory/effector Th2 cell line can be effectively attenuated by IL-35.
J Immunol. 187: 462-71. -
Kalenda, Y.D. et al. (2015) Tandem repeat recombinant proteins as potential antigens for the sero-diagnosis of Schistosoma mansoni infection.
Parasitol Int. 64 (6): 503-12. -
Kim, J.H. & Ohsawa, M. (1995) Oral tolerance to ovalbumin in mice as a model for detecting modulators of the immunologic tolerance to a specific antigen.
Biol Pharm Bull. 18 (6): 854-8. -
Ray, A. et al. (2015) Gut Microbial Dysbiosis Due to Helicobacter Drives an Increase in Marginal Zone B Cells in the Absence of IL-10 Signaling in Macrophages.
J Immunol. 195 (7): 3071-85. -
Nakano, Y. et al. (2016) Role of Prostaglandin D2 and DP1 Receptor on Japanese Cedar Pollen-Induced Allergic Rhinitis in Mice.
J Pharmacol Exp Ther. 357 (2): 258-63. -
Jeon, W.Y. et al. (2015) Soshiho-tang water extract inhibits ovalbumin-induced airway inflammation via the regulation of heme oxygenase-1.
BMC Complement Altern Med. 15 (1): 329. -
Ziegler, T. et al. (2015) A novel regulatory macrophage induced by a helminth molecule instructs IL-10 in CD4+ T cells and protects against mucosal inflammation.
J Immunol. 194 (4): 1555-64. -
Noble A & Zhao J (2016) Follicular helper T cells are responsible for IgE responses to Der p 1 following house dust mite sensitization in mice.
Clin Exp Allergy. May 3 [Epub ahead of print] -
Roberts, H. et al. (2016) Characterization of neutrophil function in Papillon-Lefèvre syndrome.
J Leukoc Biol. 100 (2): 433-44. -
Ikeda, M. et al. (2016) Beneficial effects of Galectin-9 on allergen-specific sublingual immunotherapy in a Dermatophagoides farinae-induced mouse model of chronic asthma.
Allergol Int. pii: S1323-8930(16)30161-7. [Epub ahead of print] -
González-Chomón C et al. (2016) Biomimetic contact lenses eluting olopatadine for allergic conjunctivitis.
Acta Biomater. 41: 302-11. -
Maiga, M.A. et al. (2017) Neonatal mono-colonization of germ-free mice with Lactobacillus casei enhances casein immunogenicity after oral sensitization to cow's milk.
Mol Nutr Food Res. Mar 20. [Epub ahead of print] -
J Wadley, A. et al. (2017) Heightened Exercise-Induced Oxidative Stress at Simulated Moderate Level Altitude vs. Sea Level in Trained Cyclists.
Int J Sport Nutr Exerc Metab. 27 (2): 97-104. -
Laffont S et al. (2017) Androgen signaling negatively controls group 2 innate lymphoid cells.
J Exp Med. May 8. pii: jem.20161807. -
Chesné, J. et al. (2015) Prime role of IL-17A in neutrophilia and airway smooth muscle contraction in a house dust mite-induced allergic asthma model.
J Allergy Clin Immunol. 135 (6): 1643-1643.e3. -
Di, C. et al. (2015) Basophil-associated OX40 ligand participates in the initiation of Th2 responses during airway inflammation.
J Biol Chem. 290 (20): 12523-36.
