IgE antibody | LO-ME-2
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Rat anti Mouse IgE
- Product Type
- Monoclonal Antibody
- Clone
- LO-ME-2
- Isotype
- IgG2a
- Specificity
- IgE
Antibody Quality and Performance Guarantee
| Rat anti Mouse IgE antibody, clone LO-ME-2 reacts with the epsilon heavy chain of mouse IgE. No cross reactivity is seen with other mouse immunoglobulins, as tested in an ELISA. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide (NaN3) - Immunogen
- Purified IgE from Balb/c mice.
- Affinity
- 2 x 10(e)9 L/mole.
- Approx. Protein Concentrations
- 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
References for IgE antibody
-
Kikuchi, Y. et al. (2006) Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses.
J Immunol. 177 (3): 1609-17. -
PeeblesRs, J.r. et al. (2005) Allergen-induced airway hyperresponsiveness mediated by cyclooxygenase inhibition is not dependent on 5-lipoxygenase or IL-5, but is IL-13 dependent.
J Immunol. 175 (12): 8253-9. -
Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
J Immunol. 174 (1): 525-32. -
PeeblesRs, J.r. et al. (2000) Cyclooxygenase inhibition increases interleukin 5 and interleukin 13 production and airway hyperresponsiveness in allergic mice.
Am J Respir Crit Care Med. 162 (2 Pt 1): 676-81.
- RRID
- AB_620449
- UniProt
- P06336
- Entrez Gene
- Gm900
- GO Terms
- GO:0003823 antigen binding
5118-6604
149710
157851
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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