Rat anti Mouse IgE antibody, clone LO-ME-2 reacts with the epsilon heavy chain of mouse IgE.
No cross reactivity is seen with other mouse immunoglobulins, as tested in an ELISA.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Purified IgE from Balb/c mice.
- 2 x 10(e)9 L/mole.
- Approx. Protein Concentrations
- 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IgE antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for IgE antibody
Kikuchi, Y. et al. (2006) Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses.
J Immunol. 177 (3): 1609-17.
PeeblesRs, J.r. et al. (2005) Allergen-induced airway hyperresponsiveness mediated by cyclooxygenase inhibition is not dependent on 5-lipoxygenase or IL-5, but is IL-13 dependent.
J Immunol. 175 (12): 8253-9.
Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
J Immunol. 174 (1): 525-32.
PeeblesRs, J.r. et al. (2000) Cyclooxygenase inhibition increases interleukin 5 and interleukin 13 production and airway hyperresponsiveness in allergic mice.
Am J Respir Crit Care Med. 162 (2 Pt 1): 676-81.
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