IgE antibody | LO-ME-2

Rat anti Mouse IgE

Product Type
Monoclonal Antibody
Clone
LO-ME-2
Isotype
IgG2a
Specificity
IgE

Product Code Applications Pack Size List Price Your Price Qty
5118-6604
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
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loader
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Rat anti Mouse IgE antibody, clone LO-ME-2 reacts with the epsilon heavy chain of mouse IgE.

No cross reactivity is seen with other mouse immunoglobulins, as tested in an ELISA.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
Immunogen
Purified IgE from Balb/c mice.
Affinity
2 x 10(e)9 L/mole.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
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Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
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loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
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Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
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loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
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loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for IgE antibody

  1. Kikuchi, Y. et al. (2006) Crucial commitment of proteolytic activity of a purified recombinant major house dust mite allergen Der p1 to sensitization toward IgE and IgG responses.
    J Immunol. 177 (3): 1609-17.
  2. PeeblesRs, J.r. et al. (2005) Allergen-induced airway hyperresponsiveness mediated by cyclooxygenase inhibition is not dependent on 5-lipoxygenase or IL-5, but is IL-13 dependent.
    J Immunol. 175 (12): 8253-9.
  3. Hashimoto, K. et al. (2005) Cyclooxygenase inhibition augments allergic inflammation through CD4-dependent, STAT6-independent mechanisms.
    J Immunol. 174 (1): 525-32.
  4. PeeblesRs, J.r. et al. (2000) Cyclooxygenase inhibition increases interleukin 5 and interleukin 13 production and airway hyperresponsiveness in allergic mice.
    Am J Respir Crit Care Med. 162 (2 Pt 1): 676-81.

RRID
AB_620449
UniProt
P06336
Entrez Gene
Gm900
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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