IFN Gamma antibody | XMG1.2

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1ug/ml	10ug/ml
Flow Cytometry 1		1/10	1/50

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

References for IFN Gamma antibody

1. Cherwinski, H.M. et al. (1987) Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
   J Exp Med. 166 (5): 1229-44.
2. Holz, A. et al. (2001) Constitutive beta cell expression of IL-12 does not perturb self-tolerance but intensifies established autoimmune diabetes.
   J Clin Invest. 108 (12): 1749-58.
3. Hsieh, B. et al. (1996) In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells.
   J Immunol. 156 (1): 232-7.
4. Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
   J Nutr Biochem. 20: 254-60.
5. Hsu, C.S. et al. (2006) Dietary fish oil enhances adhesion molecule and interleukin-6 expression in mice with polymicrobial sepsis.
   Br J Nutr. 96: 854-60.
6. Rountree, R.B. et al. (2011) Exosome Targeting of Tumor Antigens Expressed by Cancer Vaccines Can Improve Antigen Immunogenicity and Therapeutic Efficacy.
   Cancer Res. 71: 5235-44.
7. Pletinckx K et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
   Eur J Immunol. 45 (2): 480-91.
8. Tsagozis, P. et al. (2003) CD8(+) T cells with parasite-specific cytotoxic activity and a Tc1 profile of cytokine and chemokine secretion develop in experimental visceral leishmaniasis.
   Parasite Immunol. 25 (11-12): 569-79.
View The Latest Product References
9. ReisL, E.S. et al. (2017) Mixed Formulation of Conventional and Pegylated Meglumine Antimoniate-Containing Liposomes Reduces Inflammatory Process and Parasite Burden in Leishmania infantum-Infected BALB/c Mice.
   Antimicrob Agents Chemother. 61 (11)Oct 24 [Epub ahead of print].

Synonyms

Interferon Gamma

RRID

AB_324085

UniProt

P01580

Entrez Gene

Ifng

GO Terms

GO:0005622 intracellular

GO:0001781 neutrophil apoptosis

GO:0042742 defense response to bacterium

GO:0009615 response to virus

GO:0005125 cytokine activity

GO:0005133 interferon-gamma receptor binding

GO:0005615 extracellular space

GO:0006925 inflammatory cell apoptosis

GO:0006955 immune response

View More GO Terms

GO:0019882 antigen processing and presentation

GO:0030593 neutrophil chemotaxis

GO:0030968 endoplasmic reticulum unfolded protein response

GO:0031642 negative regulation of myelination

GO:0032735 positive regulation of interleukin-12 production

GO:0044130 negative regulation of growth of symbiont in host

GO:0042102 positive regulation of T cell proliferation

GO:0042832 defense response to protozoan

GO:0045080 positive regulation of chemokine biosynthetic process

GO:0045084 positive regulation of interleukin-12 biosynthetic process

GO:0045348 positive regulation of MHC class II biosynthetic process

GO:0045410 positive regulation of interleukin-6 biosynthetic process

GO:0045429 positive regulation of nitric oxide biosynthetic process

GO:0045893 positive regulation of transcription, DNA-dependent

GO:0048304 positive regulation of isotype switching to IgG isotypes

GO:0050718 positive regulation of interleukin-1 beta secretion

MCA1548

150115 164585

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