Rat anti Mouse Interferon gamma antibody, clone XMG1.2 recognizes murine IFN gamma. Rat anti Mouse Interferon gamma antibody, clone XMG1.2 may be used for the detection of intracellular IFN gamma using flow cytometric techniques and has been shown to block the functional activity of murine interferon gamma.
- Target Species
- Product Form
- Purified IgG - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Recombinant mouse IFN gamma.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
defense response to bacterium
response to virus
interferon-gamma receptor binding
inflammatory cell apoptosis
antigen processing and presentation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of interleukin-12 production
negative regulation of growth of symbiont in host
positive regulation of T cell proliferation
defense response to protozoan
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription, DNA-dependent
positive regulation of isotype switching to IgG isotypes
positive regulation of interleukin-1 beta secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of IFN Gamma antibody
|Flow Cytometry 1
- 1 Membrane permeablisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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Secondary Antibodies Available
Product Specific References
References for IFN Gamma antibody
Cherwinski, H.M. et al. (1987) Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
J Exp Med. 166 (5): 1229-44.
Holz, A. et al. (2001) Constitutive beta cell expression of IL-12 does not perturb self-tolerance but intensifies established autoimmune diabetes.
J Clin Invest. 108 (12): 1749-58.
Hsieh, B. et al. (1996) In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells.
J Immunol. 156 (1): 232-7.
Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
J Nutr Biochem. 20: 254-60.
Hsu, C.S. et al. (2006) Dietary fish oil enhances adhesion molecule and interleukin-6 expression in mice with polymicrobial sepsis.
Br J Nutr. 96: 854-60.
Rountree, R.B. et al. (2011) Exosome Targeting of Tumor Antigens Expressed by Cancer Vaccines Can Improve Antigen Immunogenicity and Therapeutic Efficacy.
Cancer Res. 71: 5235-44.
Pletinckx K et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
Eur J Immunol. 45 (2): 480-91.
Tsagozis, P. et al. (2003) CD8(+) T cells with parasite-specific cytotoxic activity and a Tc1 profile of cytokine and chemokine secretion develop in experimental visceral leishmaniasis.
Parasite Immunol. 25 (11-12): 569-79.
ReisL, E.S. et al. (2017) Mixed Formulation of Conventional and Pegylated Meglumine Antimoniate-Containing Liposomes Reduces Inflammatory Process and Parasite Burden in Leishmania infantum-Infected BALB/c Mice.
Antimicrob Agents Chemother. 61 (11)Oct 24 [Epub ahead of print].
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