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IFN Gamma antibody | XMG1.2

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Anti Mouse Interferon Gamma Antibody, clone XMG1.2 gallery image 1
Detection of Recombinant Mouse Interferon Gamma in Sandwich ELISA using Rat anti Mouse Interferon Gamma: Endotoxin Low (MCA1548EL) as a coating antibody and a biotinylated Rabbit anti Mouse Interferon Gamma as the detection reagent

Rat anti Mouse Interferon Gamma:Biotin

Rat anti Mouse Interferon Gamma:FITC

Rat anti Mouse Interferon Gamma:Low Endotoxin

Rat anti Mouse Interferon Gamma

Product Type
Monoclonal Antibody
Clone
XMG1.2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1548B E 0.2 mg
MCA1548F F* 0.1 mg
MCA1548EL E F* FN 0.5 mg
MCA1548 E F* 0.25 mg
Rat anti Mouse Interferon gamma antibody, clone XMG1.2 recognizes murine IFN gamma. Rat anti Mouse Interferon gamma antibody, clone XMG1.2 may be used for the detection of intracellular IFN gamma using flow cytometric techniques and has been shown to block the functional activity of murine interferon gamma.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Recombinant mouse IFN gamma.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01580 Related reagents
Entrez Gene
Ifng Related reagents
GO Terms
GO:0005622 intracellular
GO:0001781 neutrophil apoptosis
GO:0042742 defense response to bacterium
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006925 inflammatory cell apoptosis
GO:0006955 immune response
GO:0019882 antigen processing and presentation
GO:0030593 neutrophil chemotaxis
GO:0030968 endoplasmic reticulum unfolded protein response
GO:0031642 negative regulation of myelination
GO:0032735 positive regulation of interleukin-12 production
GO:0044130 negative regulation of growth of symbiont in host
GO:0042102 positive regulation of T cell proliferation
GO:0042832 defense response to protozoan
GO:0045080 positive regulation of chemokine biosynthetic process
GO:0045084 positive regulation of interleukin-12 biosynthetic process
GO:0045348 positive regulation of MHC class II biosynthetic process
GO:0045410 positive regulation of interleukin-6 biosynthetic process
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045893 positive regulation of transcription, DNA-dependent
GO:0048304 positive regulation of isotype switching to IgG isotypes
GO:0050718 positive regulation of interleukin-1 beta secretion
Regulatory
For research purposes only

Applications of IFN Gamma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25ug/ml 2.0ug/ml
Flow Cytometry 1 Neat 1/10
ELISA
Flow Cytometry 1
Functional Assays
ELISA 1ug/ml 10ug/ml
Flow Cytometry 1 1/10 1/50
  1. 1 Membrane permeablisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  1. 1 Membrane permeablisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG1 Negative Control:FITC MCA1211F 100 Tests F
Rat IgG1 Negative Control:Low Endotoxin MCA1211EL 0.5 mg C E F
Rat IgG1 Negative Control MCA1211 1 ml C E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for IFN Gamma antibody

  1. Cherwinski, H.M. et al. (1987) Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies.
    J Exp Med. 166 (5): 1229-44.
  2. Holz, A. et al. (2001) Constitutive beta cell expression of IL-12 does not perturb self-tolerance but intensifies established autoimmune diabetes.
    J Clin Invest. 108 (12): 1749-58.
  3. Hsieh, B. et al. (1996) In vivo cytokine production in murine listeriosis. Evidence for immunoregulation by gamma delta+ T cells.
    J Immunol. 156 (1): 232-7.
  4. Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.
    J Nutr Biochem. 20: 254-60.
  5. Hsu, C.S. et al. (2006) Dietary fish oil enhances adhesion molecule and interleukin-6 expression in mice with polymicrobial sepsis.
    Br J Nutr. 96: 854-60.
  6. Rountree, R.B. et al. (2011) Exosome Targeting of Tumor Antigens Expressed by Cancer Vaccines Can Improve Antigen Immunogenicity and Therapeutic Efficacy.
    Cancer Res. 71: 5235-44.
  7. Pletinckx K et al. (2015) Immature dendritic cells convert anergic nonregulatory T cells into Foxp3- IL-10+ regulatory T cells by engaging CD28 and CTLA-4.
    Eur J Immunol. 45 (2): 480-91.
  8. Tsagozis, P. et al. (2003) CD8(+) T cells with parasite-specific cytotoxic activity and a Tc1 profile of cytokine and chemokine secretion develop in experimental visceral leishmaniasis.
    Parasite Immunol. 25 (11-12): 569-79.
  9. ReisL, E.S. et al. (2017) Mixed Formulation of Conventional and Pegylated Meglumine Antimoniate-Containing Liposomes Reduces Inflammatory Process and Parasite Burden in Leishmania infantum-Infected BALB/c Mice.
    Antimicrob Agents Chemother. 61 (11)Oct 24 [Epub ahead of print].
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