Dectin-2 antibody | D2.11E4

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Rat anti Mouse Dectin-2:Low Endotoxin

Rat anti Mouse Dectin-2

Product Type
Monoclonal Antibody
Clone
D2.11E4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2415EL C F* IP 0.5 mg
MCA2415T C F* IP 25 µg
MCA2415GA C F* IP 0.1 mg
MCA2415 C F* IP 0.25 mg
Rat anti Mouse Dectin-2 antibody, clone D2.11E4 recognizes mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages.

Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.

Dectin-2 binds high mannose carbohydrates and appears to play a role in immune surveillance against fungal pathogens such as Candida albicans (Goyal et al. 2018).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Carrier Free
Yes
Immunogen
Dectin-2 (extracellular domain) Fc Fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9JKF4 Related reagents
Entrez Gene
Clec4n Related reagents
GO Terms
GO:0005515 protein binding
GO:0050832 defense response to fungus
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0050715 positive regulation of cytokine secretion
Regulatory
For research purposes only

Applications of Dectin-2 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/100
Immunohistology - Frozen
Immunoprecipitation
Flow Cytometry 1 1/25 Pack Size: 25 µg, 0.25 mg
1/50 Pack Size: 0.1 mg
1/50 Pack Size: 25 µg, 0.25 mg
1/100 Pack Size: 0.1 mg
Immunohistology - Frozen
Immunoprecipitation
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of the Fixation buffer (BUF071) and ICS Perm/Wash buffer (BUF072) for this purpose.
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of the Fixation buffer (BUF071) and ICS Perm/Wash buffer (BUF072) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Low Endotoxin MCA1212EL 0.5 mg F
Rat IgG2a Negative Control MCA1212 1 ml E F

Application Based External Images

Flow Cytometry

Functional Assays

Product Specific References

References for Dectin-2 antibody

  1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.
    Eur J Immunol. 35 (7): 2163-74.
  2. Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.
    J Biol Chem. 285: 30676-85.
  3. Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.
    J Exp Med. 208: 593-604.
  4. Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection.
    J Exp Med. 206:2037-51.
  5. McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation.
    PLoS One. 7: e45781.
  6. Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses.
    Proc Natl Acad Sci U S A. 107: 20459-64.
  7. Pugholm, L.H. et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
    Scand J Immunol. 82 (6): 498-505.
  8. Lee, M.J. et al. (2016) Phosphoinositide 3-Kinase δ Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity.
    J Immunol. 197 (1): 278-87.
  9. Xiao, Y. et al. (2016) Targeting CBLB as a potential therapeutic approach for disseminated candidiasis.
    Nat Med. Jul 18. [Epub ahead of print]
  10. Roth, S. et al. (2016) Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity
    Cell Reports. 17 (10): 2572-83.
  11. Simons, B. et al. (2017) PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen.
    J Immunol. 198 (1): 461-71.
  12. Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
    PLoS Pathog. 13 (7): e1006485.