Dectin-2 antibody | D2.11E4

Rat anti Mouse Dectin-2

Product Type
Monoclonal Antibody
Clone
D2.11E4
Isotype
IgG2a
Specificity
Dectin-2

Product Code Applications Pack Size List Price Your Price Qty
MCA2415
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F * IP 0.25 mg loader
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loader
MCA2415GA
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SDS Safety Datasheet SDS
C F * IP 0.1 mg loader
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loader
MCA2415T
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SDS Safety Datasheet SDS
C F * IP 25 µg loader
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Rat anti Mouse Dectin-2 antibody, clone D2.11E4 recognizes mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages.

Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.

Dectin-2 binds high mannose carbohydrates and appears to play a role in immune surveillance against fungal pathogens such as Candida albicans (Goyal et al. 2018).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Dectin-2 (extracellular domain) Fc Fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 1/10 1/100
Immunohistology - Frozen
Immunoprecipitation
  1. 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of the Fixation buffer (BUF071) and ICS Perm/Wash buffer (BUF072) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Rabbit F(ab')2 anti Rat IgG:FITC
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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader
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Description Rat IgG2a Negative Control

References for Dectin-2 antibody

  1. Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.
    Eur J Immunol. 35 (7): 2163-74.
  2. Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.
    J Biol Chem. 285: 30676-85.
  3. Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.
    J Exp Med. 208: 593-604.
  4. Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection.
    J Exp Med. 206:2037-51.
  5. McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation.
    PLoS One. 7: e45781.
  6. Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses.
    Proc Natl Acad Sci U S A. 107: 20459-64.
  7. Pugholm, L.H. et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
    Scand J Immunol. 82 (6): 498-505.
  8. Lee, M.J. et al. (2016) Phosphoinositide 3-Kinase δ Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity.
    J Immunol. 197 (1): 278-87.
  9. View The Latest Product References
  10. Xiao, Y. et al. (2016) Targeting CBLB as a potential therapeutic approach for disseminated candidiasis.
    Nat Med. Jul 18. [Epub ahead of print]
  11. Roth, S. et al. (2016) Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity
    Cell Reports. 17 (10): 2572-83.
  12. Simons, B. et al. (2017) PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen.
    J Immunol. 198 (1): 461-71.
  13. Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
    PLoS Pathog. 13 (7): e1006485.
  14. Griffiths, J.S. et al. (2018) Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases.
    FASEB J. 32 (6): 3385-97.
  15. Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
    PLoS Pathog. 15 (6): e1007850.
  16. Jaiswal, A.K. et al. (2020) Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.
    Immunobiology. 225 (4): 151956.

Flow Cytometry

Functional Assays

RRID
AB_567286
UniProt
Q9JKF4
Entrez Gene
Clec4n
GO Terms
GO:0005515 protein binding
GO:0050832 defense response to fungus
GO:0016021 integral to membrane
GO:0005529 sugar binding
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045087 innate immune response
GO:0050715 positive regulation of cytokine secretion
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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