Dectin-2 antibody | D2.11E4
Rat anti Mouse Dectin-2 antibody, clone D2.11E4 (MCA2415) used for the assessment of dectin-2 expression on inflammatory macrophages and neutrophils by flow cytometry.
Image caption:
Assessment of dectin-2 function on inflammatory cells.
Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/Mø (Ly-6G−). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments.
From: Citation: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012)
Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation.
PLoS ONE 7(9): e45781.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Dectin-2 antibody, clone D2.11E4 (MCA2415) used for the evaluation of dectin-2 expression on dectin-2 transduced NIH3T3 cells by flow cytometry.
Image caption:
Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization.
Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FcεRγ chain (right panel).
From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012)
Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation.
PLoS ONE 7(9): e45781.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Low endotoxin Rat anti Mouse dectin-2 antibody, clone D2.11E4 (MCA2415EL) used in a functional assay to assess dectin-2 involvement in the recognition and response to zymosan by inflammatory neutrophils and monocytic cells.
Image caption:
Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/Mø. Data represents mean±SEM of triplicates from a representative of 3 independent experiments.
From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012)
Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation.
PLoS ONE 7(9): e45781.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Dectin-2
- Product Type
- Monoclonal Antibody
- Clone
- D2.11E4
- Isotype
- IgG2a
- Specificity
- Dectin-2
Antibody Quality and Performance Guarantee
| Rat anti Mouse Dectin-2 antibody, clone D2.11E4 recognizes mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages. Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation. Dectin-2 binds high mannose carbohydrates and appears to play a role in immune surveillance against fungal pathogens such as Candida albicans (Goyal et al. 2018). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Dectin-2 (extracellular domain) Fc Fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/10 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of the Fixation buffer (BUF071) and ICS Perm/Wash buffer (BUF072) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
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| List Price | Your Price | ||||||
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| Description | Rat IgG2a Negative Control | ||||||
Source Reference
-
Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.
Eur J Immunol. 35 (7): 2163-74.
References for Dectin-2 antibody
-
Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection.
J Exp Med. 206:2037-51. -
Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses.
Proc Natl Acad Sci U S A. 107: 20459-64. -
Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.
J Biol Chem. 285: 30676-85. -
Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.
J Exp Med. 208: 593-604. -
McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation.
PLoS One. 7: e45781. -
Pugholm, L.H. et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
Scand J Immunol. 82 (6): 498-505. -
Roth, S. et al. (2016) Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity.
Cell Rep. 17 (10): 2572-83. -
Lee, M.J. et al. (2016) Phosphoinositide 3-Kinase δ Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity.
J Immunol. 197 (1): 278-87.
View The Latest Product References
-
Xiao, Y. et al. (2016) Targeting CBLB as a potential therapeutic approach for disseminated candidiasis.
Nat Med. 22 (8): 906-14. -
Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
PLoS Pathog. 13 (7): e1006485. -
Simons, B. et al. (2017) PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen.
J Immunol. 198 (1): 461-71. -
Griffiths, J.S. et al. (2018) Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases.
FASEB J. 32 (6): 3385-97. -
Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
PLoS Pathog. 15 (6): e1007850. -
Jaiswal, A.K. et al. (2020) Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.
Immunobiology. 225 (4): 151956.
- RRID
- AB_567286
- UniProt
- Q9JKF4
- Entrez Gene
- Clec4n
- GO Terms
- GO:0005515 protein binding
- GO:0050832 defense response to fungus
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045087 innate immune response
- GO:0050715 positive regulation of cytokine secretion
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