Dectin-2 antibody | D2.11E4
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Rat anti Mouse Dectin-2
- Product Type
- Monoclonal Antibody
|Product Code||Applications||Pack Size||List Price||Your Price||Qty|
|Rat anti Mouse Dectin-2 antibody, clone D2.11E4 recognizes mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages.
Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.
Dectin-2 binds high mannose carbohydrates and appears to play a role in immune surveillance against fungal pathogens such as Candida albicans (Goyal et al. 2018).
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Dectin-2 (extracellular domain) Fc Fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Flow Cytometry 1||1/10||1/100|
|Immunohistology - Frozen|
- 1Membrane permeabilization is required for this application. Bio-Rad recommends the use of the Fixation buffer (BUF071) and ICS Perm/Wash buffer (BUF072) for this purpose.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Taylor, P.R. et al. (2005) Dectin-2 is predominantly myeloid restricted and exhibits unique activation-dependent expression on maturing inflammatory monocytes elicited in vivo.
Eur J Immunol. 35 (7): 2163-74.
References for Dectin-2 antibody
Robinson, M. et al. (2009) Dectin-2 is a Syk-coupled pattern recognition receptor crucial for Th17 responses to fungal infection.
J Exp Med. 206:2037-51.
Ritter, M. et al. (2010) Schistosoma mansoni triggers Dectin-2, which activates the Nlrp3 inflammasome and alters adaptive immune responses.
Proc Natl Acad Sci U S A. 107: 20459-64.
Suram, S. et al. (2010) Pathways regulating cytosolic phospholipase A2 activation and eicosanoid production in macrophages by Candida albicans.
J Biol Chem. 285: 30676-85.
Barrett, N.A. et al. (2011) Dectin-2 mediates Th2 immunity through the generation of cysteinyl leukotrienes.
J Exp Med. 208: 593-604.
McDonald, J.U. et al. (2012) Differential dependencies of monocytes and neutrophils on dectin-1, dectin-2 and complement for the recognition of fungal particles in inflammation.
PLoS One. 7: e45781.
Pugholm, L.H. et al. (2015) In vitro Assay for Screening of Optimal Targets for Antigen-Delivery to Murine Dendritic Cells.
Scand J Immunol. 82 (6): 498-505.
Roth, S. et al. (2016) Vav Proteins Are Key Regulators of Card9 Signaling for Innate Antifungal Immunity.
Cell Rep. 17 (10): 2572-83.
Lee, M.J. et al. (2016) Phosphoinositide 3-Kinase δ Regulates Dectin-2 Signaling and the Generation of Th2 and Th17 Immunity.
J Immunol. 197 (1): 278-87.
View The Latest Product References
Xiao, Y. et al. (2016) Targeting CBLB as a potential therapeutic approach for disseminated candidiasis.
Nat Med. 22 (8): 906-14.
Chang, T.H. et al. (2017) Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.
PLoS Pathog. 13 (7): e1006485.
Simons, B. et al. (2017) PGI2 Controls Pulmonary NK Cells That Prevent Airway Sensitization to House Dust Mite Allergen.
J Immunol. 198 (1): 461-71.
Griffiths, J.S. et al. (2018) Differential susceptibility of Dectin-1 isoforms to functional inactivation by neutrophil and fungal proteases.
FASEB J. 32 (6): 3385-97.
Thompson, A. et al. (2019) The protective effect of inflammatory monocytes during systemic C. albicans. infection is dependent on collaboration between C-type lectin-like receptors.
PLoS Pathog. 15 (6): e1007850.
Jaiswal, A.K. et al. (2020) Pyruvate kinase M2 in lung APCs regulates Alternaria-induced airway inflammation.
Immunobiology. 225 (4): 151956.
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0050832 defense response to fungus
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
- GO:0045087 innate immune response
- GO:0050715 positive regulation of cytokine secretion
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