Fixation Buffer

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  • Fixation Buffer
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF071Fdatasheet pdfdatasheet pdf100 ml
    BUF071
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Fixation Buffer can be used for preparing cells which will undergo staining for intracellular cytokines or proteins. The Fixation Buffer should be used prior to permeabilization using ICS Perm/Wash Buffer (BUF072) and has been designed to produce the greatest signal to noise ratio.
    • Intended Use
    • Product Form
      Liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      Paraformaldehyde
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Resuspend cells in 0.5 ml Fixation Buffer
      2. Leave in dark for 20 min at room temperature
      It is recommended that the reagent be titrated for optimal performance for each application.

    Additional Fixation Buffer Formats

    Formats Applications Sizes available
    Fixation Buffer : Reagent F 100 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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