Fixation Buffer

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  • Fixation Buffer
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF071Fdatasheet pdfdatasheet pdf100 ml
    BUF071
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Fixation Buffer can be used for preparing cells which will undergo staining for intracellular cytokines or proteins. The Fixation Buffer should be used prior to permeabilization using ICS Perm/Wash Buffer (BUF072) and has been designed to produce the greatest signal to noise ratio.
    • Intended Use
    • Product Form
      Liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      Paraformaldehyde
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      1. Resuspend cells in 0.5 ml Fixation Buffer
      2. Leave in dark for 20 min at room temperature

      Note: It is recommended that the reagent be titrated for optimal performance for each application

    Additional Fixation Buffer Formats

    Formats Applications Sizes available
    Fixation Buffer : Reagent F 100 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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