CD9 antibody | MF1
Rat anti Mouse CD9 antibody, clone MF1 (MCA2749GA) used for the evaluation of CD9 expression in murine plasma samples by western blotting.
Image caption:
CD9 and CD163 Western blot analysis of mouse plasma before (total serum) and after (non-EV fraction) precipitation of EVs (EV-fraction).
From: Etzerodt A, Berg RM, Plovsing RR, Andersen MN, Bebien M, Habbeddine M, Lawrence T, Møller HJ, Moestrup SK.
Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma.
Sci Rep. 2017 Jan 13;7:40286.
Rat anti Mouse CD9
- Product Type
- Monoclonal Antibody
- Clone
- MF1
- Isotype
- IgG2b
- Specificity
- CD9
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD9 antibody, clone MF1 recognizes mouse CD9, a ~25 kDa member of the tetraspanin family of transmembrane proteins. The tetraspanin family are multifunctional molecules which bind to a wide range of transmembrane proteins and intracellular signalling molecules. Tetraspanins facilitate the formation of molecular complexes which play a role in cell activation, proliferation, motility, fusion and apoptosis. CD9 is ubiquitously expressed, with high levels found on platelets and in male reproductive tissues. In mouse haematopoietic tissues, CD9 is expressed by myeloid and stromal cells. CD9 is known to complex with integrins and other transmembrane proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation. |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunoprecipitation | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2749 detects a band of approximately 25kDa in cell lysates.
References for CD9 antibody
-
Etzerodt, A. et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma.
Sci Rep. 7: 40286.
- RRID
- AB_10897004
- UniProt
- P40240
- Entrez Gene
- Cd9
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0006928 cellular component movement
- GO:0007342 fusion of sperm to egg plasma membrane
- GO:0008285 negative regulation of cell proliferation
- GO:0030913 paranodal junction assembly
MCA2749
150667
164446
MCA2749GA
152627
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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