CD9 antibody | MF1

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Rat anti Mouse CD9:Alexa Fluor®488

Rat anti Mouse CD9

Product Type
Monoclonal Antibody
Clone
MF1
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2749A488
Datasheet
F
SDS
100 Tests/1ml loader

MCA2749GA
Datasheet
F IP WB
SDS
0.1 mg loader

MCA2749
Datasheet
F IP WB
SDS
0.25 mg loader

Rat anti Mouse CD9 antibody, clone MF1 recognizes mouse CD9, a ~25 kDa member of the tetraspanin family of transmembrane proteins. The tetraspanin family are multifunctional molecules which bind to a wide range of transmembrane proteins and intracellular signalling molecules. Tetraspanins facilitate the formation of molecular complexes which play a role in cell activation, proliferation, motility, fusion and apoptosis.

CD9 is ubiquitously expressed, with high levels found on platelets and in male reproductive tissues. In mouse haematopoietic tissues, CD9 is expressed by myeloid and stromal cells. CD9 is known to complex with integrins and other transmembrane proteins. It can modulate cell adhesion and migration and also trigger platelet activation and aggregation.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor 488 - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P40240
Entrez Gene
Cd9
GO Terms
GO:0005515 protein binding
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0006928 cellular component movement
GO:0007342 fusion of sperm to egg plasma membrane
GO:0008285 negative regulation of cell proliferation
GO:0030913 paranodal junction assembly
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD9 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A or BUF041B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Western Blotting
MCA2749 detects a band of approximately 25kDa in cell lysates.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD9 antibody

  1. Etzerodt, A. et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of 'soluble CD163' in plasma.
    Sci Rep. 7: 40286.