Gr-1 antibody | RB6-8C5
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.
Image caption:
Depletion of the circulating monocyte and Gr1lo and F4/80hi populations following 1 week GW2580 treatment. A. Identification of total monocyte population in mouse blood using MAFIA-GFP. B Following one week of GW2580 treatment, no difference in total circulating monocytes was observed. FACS quantification of Gr1hi (M1) and Gr1lo (M2; C,D) and F4/80hi (E,F) in total monocytes following 1 week GW2580 treatment (n = 6 and n = 4 animals per group, respectively).
From: Leblond A-L, Klinkert K, Martin K, Turner EC, Kumar AH, Browne T, et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS ONE 10(9): e0137515.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the identification of neutrophils by immunofluorescence.
Image caption:
S. typhimurium-Infected Macrophages Containing Phagocytosed Neutrophils and T Cells Confocal fluorescence microscopy of 50-μm-thick liver sections from 1-wk-infected Slc11a1 wild-type mice. (A–C) S. Typhimurium (O-antigen, arrows) are red, macrophages (F4–80 and MOMA-2) are blue, DNA (DAPI) is gray, phalloidin is green, and neutrophils (Gr-1/Ly-6G/RB6-8C5) are pink (arrowheads). (A) Collapsed image from a 40-μm Z-stack. Scale bar is 20 μm. (B and C) Sections from (A) that are 4 μm apart. The video from which (A–C) were derived (Video S2) is available online. (D–G) T cells within multinucleate macrophages. Macrophages (F4–80 and MOMA-2) are blue (D, G, and H), T cells (CD3zeta) are red (D, G, arrowheads), DAPI is gray (E, G), actin-bound phalloidin is green (F, G). (G) Is a composite of (D, E, and F). Scale bars are 16 μm. (H) An image from a different mouse stained and labeled as described for (D–G). Scale bar is 8 μm. A video showing a T cell inside of a macrophage is available online (Video S3).
From: Nix RN, Altschuler SE, Henson PM, Detweiler CS (2007) Hemophagocytic Macrophages Harbor Salmonella enterica during Persistent Infection.
PLoS Pathog 3(12): e193.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody (MCA2387) used for the visualisation of infiltrating granulocytes in a murine model of ureteral obstruction induced kidney fibrosis by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Bone marrow-derived cells induced by LTB4-BLT1 signaling exacerbate renal fibrosis in UUO kidneys.
We examined renal fibrosis in UUO kidneys of WT mice transplanted with BLT1-/-BM (BLT1-/-BM→WT), and compared it with that in WT mice transplanted WT-BM (WT-BM→WT).(E) Representative images showing F4/80 immunostaining in UUO kidneys of WT-BM→WT and BLT1-/-BM→WT at Day 7. Scale bars = 100μm. (F) Number of F/80-positive cells in UUO kidneys of WT-BM→WT and BLT1-/-BM→WT. Data are expressed as the mean ± SEM (n = 4 mice/group). **P<0.01, vs. WT-BM→WT. (G) Representative images showing Gr1 immunostaining in UUO kidneys of WT-BM→WT and BLT1-/-BM→WT at Day 7. Scale bars = 100μm. (H) Number of Gr1-positive cells in UUO kidneys of WT-BM→WT and BLT1-/-BM→WT. Data are expressed as the mean ±SEM (n = 4 mice/group). **P<0.01, vs. WT-BM→WT.
From: Kamata M, Amano H, Ito Y, Fujita T, Otaka F, Hosono K, et al. (2019)Role of the high-affinity leukotriene B4 receptor signaling in fibrosis after unilateral ureteral obstruction in mice.
PLoS ONE 14(2): e0202842.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used for the identification of infiltrating neutrophils in murine lung tissue sections by immunofluorescence
Image caption:
From: Natanov R, Gueler F, Falk CS, Kühn C, Maus U, Boyle EC, et al. (2018)
Blood cytokine expression correlates with early multi-organ damage in a mouse model of moderate hypothermia with circulatory arrest using cardiopulmonary bypass.
PLoS ONE 13(10): e0205437.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 used for the evaluation of Gr-1 expression on circulating monocytes from mouse blood by flow cytometry.
Image caption:
Effect of CD206+ M2 macrophage depletion on collagen deposition and cell infiltration within the infarct 2 weeks post MI in MAFIA mice. A. Representative Sirius Red staining on histological sections from MAFIA mice 2 weeks post MI. B. Quantification of collagen staining as a percentage of LV from MAFIA mice 2 weeks post MI (Scale bar: 1 mm, n = 6 animals per group, ≥8 images per animal). C. Representative hematoxilin and eosin staining on 20x infarct or remote histological section from MAFIA mice 2 weeks post MI showing an increase in inflammatory infiltrates in animals treated with GW2580. D. Quantification of nuclei number per mm2 in MAFIA remote and infarct zone in MAFIA mice 2 weeks post MI. (n = 4–6 animals per group). E. Representative images of immunofluorescent staining of CD206+ M2 macrophages, Gr1+ M1 macrophages and Ly6G+ neutrophils within the infarct zone from MAFIA mice 2 weeks post MI. Quantification of Ly6G+ neutrophil, Gr1+ M1 macrophage and CD206+ M2 macrophage infiltration within the infarct zone from MAFIA mice 2 weeks post MI (n = 4 animals per group, 10 images/animal). * p<0.05, ** p<0.01.
From: Leblond A-L, Klinkert K, Martin K, Turner EC, Kumar AH, Browne T, et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS ONE 10(9): e0137515.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
RPE conjugated Rat anti Mouse GR-1 antibody, clone RB6-8C5 (MCA2387PE) used for the evaluation of GR-1 (Ly6G) expression on murine uterine derived neutrophils by flow cytometry.
Image caption:
Immunophenotyping of uterine macrophages 24 hours after withdrawal of progesterone.
GFP+ cell populations present in the uterus were characterised by flow cytometry of tissue digested from both control (non-stimulated/non-decidualized) and decidualized horns. (A) In MacGreen mice, few GFP+ cells were detected in non-stimulated uterine horns (non), which was in marked contrast to the abundant GFP+ cells in the decidualized horn (stim). (B) Analysis of proportion of influxing cells revealed a significant increase in GFP+ cell populations (p < 0.001) in stimulated uterine horns (n = 7). GFP+ cells (see A) were gated for further analysis and expression of F4/80 and Gr-1 was assessed. (C) The majority of GFP+ cells were F4/80+ and almost all expressed Gr-1. Isolated GFP+ cells had mononuclear morphology as determined by immunofluorescence (green; GFP, blue; DAPI). (D) F4/80 expression within the GFP+ cell population was detected in the majority of cells (n = 5, p < 0.01). (E) F4/80 and Gr-1 expression in non- and stimulated horns in wildtype (WT) mice was assessed. (F) In WT mice Gr-1+ cells were significantly increased in stimulated compared to control horns (n = 4–6, p < 0.01). (G) F4/80+ Gr-1− populations were unchanged in non and stimulated uterine horns but the proportion of total F4/80+ cells was increased in stimulated compared to control horns (n = 5–6, p < 0.01). (H) The majority of influxing Gr-1+ cells were F4/80+ (n = 5, p < 0.01).
From: Cousins FL, Kirkwood PM, Saunders PT, Gibson DA.
Evidence for a dynamic role for mononuclear phagocytes during endometrial repair and remodelling.
Sci Rep. 2016 Nov 9;6:36748.
doi: 10.1038/srep36748.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse GR-1 antibody, clone RB6-8C5 (MCA2387GA) used for the identification of murine neutrophils in liver sections by immunofluorescence.
Image caption:
Hepatic neutrophils in WT and BLT1−/−sup> mice during APAP hepatotoxicity.
(A) Representative images of liver sections in immunostaining analysis with an anti- Gr-1 antibody in WT mice (upper panel) and BLT1-/- mice (lower panel) at 24 h after APAP administration. Arrow heads indicate Gr-1-positive cells. CV, central vein. Bars = 50 μm. (B) Changes in the total numbers of cells immunopositive for Gr-1 (neutrophils) in the livers of WT and BLT1−/− mice after APAP administration. Data are expressed as the means ± SEM of 5–6 mice per group. *p < 0.05 vs. WT mice. (C) Changes in the numbers of extra-sinusoidal neutrophils in the livers of WT and BLT1−/− mice after APAP administration. Data are expressed as the means ± SEM of 5–6 mice per group. *p < 0.05 vs. WT mice.
From: Kojo K, Ito Y, Eshima K, Nishizawa N, Ohkubo H, Yokomizo T, Shimizu T, Watanabe M, Majima M.
BLT1 signalling protects the liver against acetaminophen hepatotoxicity by preventing excessive accumulation of hepatic neutrophils.
Sci Rep. 2016 Jul 11;6:29650.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to evaluate neutrophil infiltration in murine lung by immunofluorescence.
Image caption:
MMP14 blockade inhibits inflammation and neutrophil adhesion.
Representative lung immunostaining for granulocyte differentiation antigen (Gr)−1 (red) was performed 16 hr after cecal ligation puncture (CLP) or sham surgery in E2C6 (10 mg/kg) or IgG control antibody (IgG)-treated mice (nuclear staining with 4′,6-diamidino-2-phenylindole, (blue), autofluorescence is shown in green). (n = 5) Scale bar 50 μm.
From: Idowu TO, Etzrodt V, Seeliger B, Bolanos-Palmieri P, Thamm K, Haller H, David S.
Identification of specific Tie2 cleavage sites and therapeutic modulation in experimental sepsis.
Elife. 2020 Aug 24;9:e59520.
doi: 10.7554/eLife.59520.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to identify neutrophils in murine heart tissue by immunofluorescence.
Image caption:
Immunofluorescence staining of neutrophils infiltrated in the heart tissue.
Following 45 min LAD occlusion and 24-h reperfusion, hearts from WT and CARD9-/- mice were cryo-sectioned (7 μm) and stained with antibodies against GR-1 for neutrophils (red) and DAPI for nuclei (blue). A, Representative heart sections showing GR-1, DAPI and merged staining images of neutrophils and nuclei; B, Analyses of the number of neutrophils as percentage of the total cell nuclei in the section. The number of infiltrated neutrophils in the WT mouse heart was significantly higher than that in the CARD9-/- mouse heart. Mean ± SEM, n = 3/group, **p <0.01 vs. WT.
From: Qin X, Peterson MR, Haller SE, Cao L, Thomas DP, He G (2018)
Caspase recruitment domain-containing protein 9 (CARD9) knockout reduces regional ischemia/reperfusion injury through an attenuated inflammatory response.
PLoS ONE 13(6): e0199711.
doi: 10.1371/journal.pone.0199711
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA497R) used to detect neutrophils in mouse skin by immunofluorescence.
Image caption:
Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 4), anti-K6 (n = 3), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA497R) used to detect neutrophils in mouse skin by immunofluorescence.
Image caption:
Representative images from skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 5), anti-K6 (n = 5), anti-F4/80 (n = 5) and anti-Gr-1 (n = 5) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA497R) used to detect neutrophils in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 4), anti-K6 (n = 3), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA497R) used to detect neutrophils in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with anti-K10, anti-K14 (n = 5), anti-K6 (n = 5), anti-F4/80 (n = 3) and anti-Gr-1 (n = 3) antibodies and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA497R) used to detect neutrophils in mouse skin by immunofluorescence.
Image caption:
Representative images of skin sections from 3-week-old mice of the indicated genotypes, immunostained with antibodies against keratin 10 (K10), keratin 14 (K14) (n = 6), keratin 6 (K6) (n = 6), F4/80 (n = 6) and Gr-1 (n = 6) and counterstained with DAPI (DNA stain). Scale bars: K10, K14, K6 = 20 μm; F4/80, Gr-1 = 30 μm.
From: Schünke H, Göbel U, Dikic I, Pasparakis M.
OTULIN inhibits RIPK1-mediated keratinocyte necroptosis to prevent skin inflammation in mice.
Nat Commun. 2021 Oct 8;12(1):5912.
doi: 10.1038/s41467-021-25945-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to label neutrophils in mouse hepatic tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Neutrophil accumulation in the liver after bile duct ligation (BDL).
Ly-6G immunohistochemical staining. Neutrophils accumulated in peribiliary areas after BDL. CXCR2−/− mice showed significant less neutrophil accumulation around biliary structures than wild-type mice. Data are means ± SE. Sham: wild-type n = 3, CXCR2−/− n = 3; 3 days: wild-type n = 7, CXCR2−/− n = 6; and 14 days: wild-type n = 9, CXCR2−/− n = 7. Student’s t test was used for the statistical analysis. *P <0.05, compared with wild type. Original magnification: ×200.
From: Konishi T, Schuster RM, Goetzman HS, Caldwell CC, Lentsch AB.
Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
Am J Physiol Gastrointest Liver Physiol. 2019 Dec 1;317(6):G773-G783.
doi: 10.1152/ajpgi.00080.2019.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to label neutrophils in mouse hepatic tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Neutrophil accumulation in the liver after bile duct ligation (BDL). Ly-6G immunohistochemical staining. A: neutrophils accumulated around focal necrosis after BDL. CXC chemokine receptor 2 knockout (CXCR2−/−; CXCR2KO) mice showed significant less neutrophil accumulation around focal necrosis than wild-type (WT) mice. HPF, high-power field. Original magnification: ×400. Data are means ± SE. Sham: wild-type n = 3, CXCR2−/− n = 3; 3 days: wild-type n = 7, CXCR2−/− n = 6; and 14 days: wild-type n = 9, CXCR2−/− n = 7. Student’s t test was used for the statistical analysis. *P <0.05, compared with wild type. Original magnification: ×200.
From: Konishi T, Schuster RM, Goetzman HS, Caldwell CC, Lentsch AB.
Cell-specific regulatory effects of CXCR2 on cholestatic liver injury.
Am J Physiol Gastrointest Liver Physiol. 2019 Dec 1;317(6):G773-G783.
doi: 10.1152/ajpgi.00080.2019
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387GA) used to label neutrophils in the tail vertebrae of mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
The cellular composition of the TgA86 axial pathology involves mesenchymal and osteoblastic lineage cells. B220-positive cells form aggregates in the bone marrow cavity, while Gr1-positive cells accumulate at the sites of enthesis of 20-week-old TgA86 mice
From: Christodoulou-Vafeiadou E, Geka C, Ntari L, Kranidioti K, Argyropoulou E, Meier F, Armaka M, Mourouzis I, Pantos C, Rouchota M, Loudos G, Denis MC, Karagianni N, Kollias G.
Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis.
Arthritis Res Ther. 2020 Oct 6;22(1):232.
doi: 10.1186/s13075-020-02327-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387GA) used to label neutrophils in the aortic valves of mice by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Histopathological analysis of the TgA86 hearts reveal comorbid aortic heart valve dysfunction.
Representative immunohistochemical stainings of aortic valve sections of wt and TgA86 mice at the age of 20 weeks with anti-Vimentin and anti-Gr1 antibodies (A) reveal that the thickened areas of the valve are mainly composed by Vimentin+ resident fibroblasts as well as few Gr1+ infiltrated neutrophils.
From: Christodoulou-Vafeiadou E, Geka C, Ntari L, Kranidioti K, Argyropoulou E, Meier F, Armaka M, Mourouzis I, Pantos C, Rouchota M, Loudos G, Denis MC, Karagianni N, Kollias G.
Ectopic bone formation and systemic bone loss in a transmembrane TNF-driven model of human spondyloarthritis.
Arthritis Res Ther. 2020 Oct 6;22(1):232.
doi: 10.1186/s13075-020-02327-4.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to label neutrophils in murine kidneys by immunofluorescence.
Image caption:
Renal neutrophil infiltration three days after renal ischemia–reperfusion injury (IRI) and repetitive diclofenac treatment and CXCL2 expression after a single oral dose of diclofenac. Gr-1 stain for neutrophils was performed on kidney sections of IRI mice treated with vehicle (A), or repetitive doses of 100 mg diclofenac (B) over three days are shown. Sham-operated mice treated with 100 mg diclofenac over three days (C) and vehicle-treated sham mice (D) served as control. Semi-quantitative scoring of renal neutrophil infiltration is shown in E. Repetitive doses of diclofenac after IRI significantly increased renal neutrophil infiltration. Diclofenac treatment in the setting of sham surgery did not cause significant neutrophil infiltration compared with sham surgery with vehicle treatment (E). In line, CXCL2, the chemoattractant for neutrophils, is upregulated in the kidney tissue in subclinical AKI and single-dose diclofenac treatment in a dose-dependent manner (F). n = 4–5 mice in each group are shown. ** p <0.01. Scale bar 100μm.
From: Störmer J, Gwinner W, Derlin K, Immenschuh S, Rong S, Jang MS, Shushakova N, Haller H, Gueler F, Greite R.
A Single Oral Dose of Diclofenac Causes Transition of Experimental Subclinical Acute Kidney Injury to Chronic Kidney Disease.
Biomedicines. 2022 May 22;10(5):1198.
doi: 10.3390/biomedicines10051198.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1 antibody, clone RB6-8C5 (MCA2387) used to label neutrophils in mouse kidney by immunofluorescence.
Image caption:
Renal morphology (PAS staining, (A,C)) and neutrophil infiltration (immunofluorescence for Gr-1 (B,D)) in mice 48 h after IRI with and without CFH treatment. The renal IRI model used in this study was characterized by only minor morphological kidney damage (A,E). However, CFH administration in renal IRI mice caused severe acute tubular injury (ATI) with loss of brush border, tubular dilatation, and tubular cast formation (C,E). After renal IRI and vehicle treatment, minor renal neutrophil infiltration was observed (B,F). In contrast, severe neutrophil infiltration was present 48 h after IRI and CFH treatment (D,F). * p < 0.05, *** p < 0.001. Scale bar 100 μm.
From: Greite R, et al.
Cell-Free Hemoglobin in Acute Kidney Injury after Lung Transplantation and Experimental Renal Ischemia/Reperfusion.
Int J Mol Sci. 2022 Oct 31;23(21):13272.
doi: 10.3390/ijms232113272.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse Gr-1:RPE
- Product Type
- Monoclonal Antibody
- Clone
- RB6-8C5
- Isotype
- IgG2b
- Specificity
- Gr-1
Antibody Quality and Performance Guarantee
| Rat anti Mouse Gr-1 antibody, clone RB6-8C5 recognizes the mouse Gr-1 antigen, a ~21–25 kDa GPI anchored cell surface protein bearing a single uPAR/Ly6 domain that belongs to the Ly-6 family of proteins (Lee et al. 2013). Rat anti Mouse Gr-1 antibody, clone RB6-8C5 reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been reported (Fleming et al. 1993). However, other observations dispute the cross-reactivity of clone RB6-8C5 with the Ly-6C protein with the alternative explanation that certain sub-populations of bone marrow cells simultaneously express both Ly-6C and Ly-6G (Nagendra et al. 2007) The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Rat anti Mouse Gr-1 antibody, clone RB6-8C5 has been used successfully for the depletion of mature neutrophils in vivo (Czuprynski et al 1994, Daley et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose - Immunogen
- Normal murine bone marrow cells.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for Gr-1 antibody
-
Hestdal, K. et al. (1991) Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells.
J Immunol. 147 (1): 22-8. -
Fleming, T.J. et al. (1993) Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family.
J Immunol. 151 (5): 2399-408. -
Czuprynski, C.J. et al. (1994) Administration of anti-granulocyte mAb RB6-8C5 impairs the resistance of mice to Listeria monocytogenes infection.
J Immunol. 152 (4): 1836-46. -
Conlan, J. and North, R. (1994) Neutrophils are essential for early anti-Listeria defense in the liver, but not in the spleen or peritoneal cavity, as revealed by a granulocyte-depleting monoclonal antibody.
J Exp Med. 179:259-68. -
Heckelsmiller, K. et al. (2002) Combined dendritic cell- and CpG oligonucleotide-based immune therapy cures large murine tumors that resist chemotherapy.
Eur J Immunol. 32 (11): 3235-45. -
Suttmann, H. et al. (2006) Neutrophil granulocytes are required for effective Bacillus Calmette-Guérin immunotherapy of bladder cancer and orchestrate local immune responses.
Cancer Res. 66: 8250-7. -
Nix, R.N. et al. (2007) Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.
PLoS Pathog. 3: e193. -
Sumagin R et al. (2010) LFA-1 and Mac-1 define characteristically different intralumenal crawling and emigration patterns for monocytes and neutrophils in situ.
J Immunol. 185 (11): 7057-66.
View The Latest Product References
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Giroux, M. et al. (2011) SMAD3 prevents graft-versus-host disease by restraining Th1 differentiation and granulocyte-mediated tissue damage.
Blood.117: 1734-44. -
Francke, A. et al. (2011) Generation of mature murine monocytes from heterogeneous bone marrow and description of their properties.
J Histochem Cytochem. 59: 813-25. -
Takano, K. et al. (2011) Successful treatment of acute lung injury with pitavastatin in septic mice: potential role of glucocorticoid receptor expression in alveolar macrophages.
J Pharmacol Exp Ther. 336: 381-90. -
Kanda, N. et al. (2011) Visfatin Enhances CXCL8, CXCL10, and CCL20 Production in Human Keratinocytes.
Endocrinology. 152: 3155-64. -
Sharp, P.E. et al. (2013) FcγRIIb on myeloid cells and intrinsic renal cells rather than B cells protects from nephrotoxic nephritis.
J Immunol.190: 340-8. -
Takebe, M. et al. (2014) Inhibition of histone deacetylases protects septic mice from lung and splenic apoptosis.
J Surg Res. 187 (2): 559-70. -
Hamers, A.A. et al. (2014) Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis.
Infect Immun. 82 (1): 253-64. -
Leblond, A.L. et al. (2015) Systemic and Cardiac Depletion of M2 Macrophage through CSF-1R Signaling Inhibition Alters Cardiac Function Post Myocardial Infarction.
PLoS One. 10 (9): e0137515. -
Wang, Y. et al. (2015) Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
Kidney Int. 88 (6): 1274-1282. -
Zhang, M.Z. et al. (2015) Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension.
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Infect Immun. 83 (8): 3257-67. -
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- Synonyms
- Ly-6G
- RRID
- AB_808629
- UniProt
- P35461
- Entrez Gene
- Ly6g
- GO Terms
- GO:0009897 external side of plasma membrane
- GO:0031225 anchored to membrane
MCA2387PE
149916
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