CD80 antibody | RM80

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Rat anti Mouse CD80:Alexa Fluor® 488

Rat anti Mouse CD80:Alexa Fluor® 647

Rat anti Mouse CD80:FITC

Rat anti Mouse CD80

Rat anti Mouse CD80:RPE

Product Type
Monoclonal Antibody
Clone
RM80
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2462A488 F 100 Tests/1ml
MCA2462A647T F 25 Tests/0.25ml
MCA2462A647 F 100 Tests/1ml
MCA2462FT F 25 µg
MCA2462F F 0.1 mg
MCA2462T C F 25 µg
MCA2462GA C F 0.1 mg
MCA2462 C F 0.25 mg
MCA2462PET F 25 Tests
MCA2462PE F 100 Tests
Rat anti Mouse CD80 antibody, clone RM80 recognizes mouse CD80 (B7-1), a ~60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells, and expression may be significantly increased upon B lymphocytes by LPS and by IL-4.

CD80 has been identified as a ligand for CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4), two structurally similar molecules expressed on T cells. CD28 and CTLA4 are two receptors that have essential but opposing functions in T-cell stimulation. The Interaction of CD80 with CD28 stimulates and sustains T cell responses, whereas the interaction of CD80 with CTLA4 is reported to inhibit T-cell responses.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Pack Size: 25 Tests
Reconstitute in 0.25 ml disilled water
Pack Size: 100 Tests
Reconstitute with 1.0ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
5%Sucrose
Carrier Free
Yes
Immunogen
BCL1 cells expressing CD80.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised SD rats were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of reconstitution

More Information

UniProt
Q00609 Related reagents
Entrez Gene
Cd80 Related reagents
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0031295 T cell costimulation
GO:0046641 positive regulation of alpha-beta T cell proliferation
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD80 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Flow Cytometry Neat 1/5
Flow Cytometry Neat
Flow Cytometry 1/10 1/50
Immunohistology - Frozen
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488 MCA1212A488 100 Tests/1ml F
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 100 Tests/1ml F
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for CD80 antibody

  1. Nuriya, S. et al. (1996) The differential role of CD86 and CD80 co-stimulatory molecules in the induction and the effector phases of contact hypersensitivity.
    Int Immunol. 8 (6): 917-26.
  2. Jin LP et al. (2004) Adoptive transfer of paternal antigen-hyporesponsive T cells induces maternal tolerance to the allogeneic fetus in abortion-prone matings.
    J Immunol. 173 (6): 3612-9.
  3. Nakajima, A. et al. (1997) Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells.
    Int Immunol. 9 (5): 637-44.
  4. Nozawa, K. et al. (2001) Preferential blockade of CD8(+) T cell responses by administration of anti-CD137 ligand monoclonal antibody results in differential effect on development of murine acute and chronic graft-versus-host diseases.
    J Immunol. 167 (9): 4981-6.
  5. Bedoret, D. et al. (2009) Lung interstitial macrophages alter dendritic cell functions to prevent airway allergy in mice.
    J Clin Invest. 119 (12): 3723-38.
  6. Legutko, A. et al. (2011) Sirtuin 1 Promotes Th2 Responses and Airway Allergy by Repressing Peroxisome Proliferator-Activated Receptor-{gamma} Activity in Dendritic Cells.
    J Immunol. 187: 4517-29.