CD8 Alpha antibody | KT15

Rat anti Mouse CD8 Alpha

Product Type
Monoclonal Antibody
Clone
KT15
Isotype
IgG2a
Specificity
CD8 Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA609G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 0.25 mg loader
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loader
MCA609GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 0.1 mg loader
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loader
MCA609GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C * F IF 25 µg loader
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loader
Search for Batch Specific Datasheets

Rat anti Mouse CD8α, clone KT15, recognizes the alpha chain of mouse CD8. CD8 is a heterodimeric protein composed of disulphide-linked CD8α and CD8β chains that is expressed primarily on cytotoxic T-cells. CD8 functions in the interaction with MHC Class I-bearing targets and plays a role in T-cell-mediated killing (Nakauchi , H. et al., 1985 & Nakauchi, H. et al., 1987).

Clone KT15 is reported to block T-cell-mediated cytotoxicity in in vitro assays (Zeis, M. et al., 2002).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
T cell clone, C6
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized SD rats were fused with cells of the NS0 mouse myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 Pack Size: 0.25 mg, 25 µg
1/100 Pack Size: 0.1 mg
1/100 Pack Size: 0.25 mg, 25 µg
1/200 Pack Size: 0.1 mg
Immunofluorescence
Immunohistology - Frozen 1
Immunohistology - Paraffin
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Rabbit F(ab')2 anti Rat IgG:HRP
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Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)

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Description Rat IgG2a Negative Control

References for CD8 Alpha antibody

  1. Tomonari, K. & Lovering, E. (1988) T-cell receptor-specific monoclonal antibodies against a V beta 11-positive mouse T-cell clone.
    Immunogenetics. 28 (6): 445-51.
  2. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  3. Lee, Y.L. et al (2003) Oral administration of Agaricus blazei (H1 strain) inhibited tumor growth in a sarcoma 180 inoculation model.
    Exp Anim. 52: 371-5.
  4. Eller, K. et al. (2011) IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression.
    J Immunol. 186: 83-91.
  5. Grimm, M. et al. (2010) Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases.
    BMC Cancer. 10: 82.
  6. Liao, D. et al. (2009) Cancer Associated Fibroblasts Promote Tumor Growth and Metastasis by Modulating the Tumor Immune Microenvironment in a 4T1 Murine Breast Cancer Model
    PLoS One. 4: e7965.
  7. Moos, M.P. et al. (2005) The lamina adventitia is the major site of immune cell accumulation in standard chow-fed apolipoprotein E-deficient mice.
    Arterioscler Thromb Vasc Biol. 25: 2386-91.
  8. Stevenson, P.G. et al. (2002) Uncoupling of virus-induced inflammation and anti-viral immunity in the brain parenchyma.
    J Gen Virol. 83: 1735-43.
  9. View The Latest Product References
  10. Wang, X. et al. (2011) Quercetin and Bornyl Acetate Regulate T-Lymphocyte Subsets and INF-γ/IL-4 Ratio In Utero in Pregnant Mice.
    Evid Based Complement Alternat Med. 2011: 745262.
  11. Zeis, M. et al. (2002) Idiotype protein-pulsed dendritic cells produce strong anti-myeloma effects after syngeneic stem cell transplantation in mice.
    Bone Marrow Transplant. 29: 213-21.
  12. Ideguchi, M. et al. (2008) Immune or inflammatory response by the host brain suppresses neuronal differentiation of transplanted ES cell-derived neural precursor cells.
    J Neurosci Res. 86: 1936-43.
  13. Wolf, D. et al. (2005) CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice.
    J Am Soc Nephrol. 16: 1360-70.
  14. Severinova, J. et al. (2005) Co-inoculation of Borrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice.
    Folia Microbiol (Praha). 50: 457-63.
  15. Zaini, J. et al. (2007) OX40 ligand expressed by DCs costimulates NKT and CD4+ Th cell antitumor immunity in mice.
    J Clin Invest. 117: 3330-8.
  16. Meyer, C. et al. (2011) Chronic inflammation promotes myeloid-derived suppressor cell activation blocking antitumor immunity in transgenic mouse melanoma model.
    Proc Natl Acad Sci U S A. 108: 17111-6.
  17. Zitt, E. et al. (2011) The selective mineralocorticoid receptor antagonist eplerenone is protective in mild anti-GBM glomeru-lonephritis.
    Int J Clin Exp Pathol. 4:606-15.
  18. Singh, V. et al. (2011) Co-administration of IL-1+IL-6+TNF-α with Mycobacterium tuberculosis infected macrophages vaccine induces better protective T cell memory than BCG.
    PLoS One. 6: e16097.
  19. Kalyanasundaram Bhanumathy, K. et al. (2015) Potent immunotherapy against well-established thymoma using adoptively transferred transgene IL-6-engineered dendritic cell-stimulated CD8(+) T-cells with prolonged survival and enhanced cytotoxicity.
    J Gene Med. 17 (8-9): 153-60.
  20. Abiko K et al. (2015) IFN-γ from lymphocytes induces PD-L1 expression and promotes progression of ovarian cancer.
    Br J Cancer. 112 (9): 1501-9.
  21. Phan-Lai, V. et al. (2016) The Antitumor Efficacy of IL2/IL21-Cultured Polyfunctional Neu-Specific T Cells Is TNFα/IL17 Dependent.
    Clin Cancer Res. 22 (9): 2207-16.
  22. Kajiwara, T. et al. (2016) Hypoxia augments MHC class I antigen presentation via facilitation of ERO1-α-mediated oxidative folding in murine tumor cells.
    Eur J Immunol. Sep 26. [Epub ahead of print]
  23. Srivastava, A.K. et al. (2016) Co-transplantation of syngeneic mesenchymal stem cells improves survival of allogeneic glial-restricted precursors in mouse brain.
    Exp Neurol. 275 Pt 1: 154-61.
  24. Meier, R.P. et al. (2014) Survival of free and encapsulated human and rat islet xenografts transplanted into the mouse bone marrow.
    PLoS One. 9 (3): e91268.
  25. Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
    Brain Communications. 3(2): fcab047.
  26. Cecil, D.L. et al. (2022) COX-2 inhibitors decrease expression of PD-L1 in colon tumors and increase the influx of Type I tumor infiltrating lymphocytes.
    Cancer Prev Res (Phila). canprevres.0227.2021.
  27. Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
    Brain Behav Immun. 101: 194-210.

Flow Cytometry

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
LY-2
RRID
AB_321407
UniProt
P01731
Entrez Gene
Cd8a
GO Terms
GO:0002456 T cell mediated immunity
GO:0016021 integral to membrane
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0042803 protein homodimerization activity
GO:0045065 cytotoxic T cell differentiation
GO:0050850 positive regulation of calcium-mediated signaling
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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