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CD4 antibody | RM4-5

Rat anti Mouse CD4:StarBright Violet 440

Product Type
Monoclonal Antibody
Clone
RM4-5
Isotype
IgG2a
Specificity
CD4

Product Code Applications Pack Size List Price Your Price Qty
MCA2691SBV440
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Rat anti Mouse CD4 antibody, clone RM4-5 detects mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance.

Rat anti Mouse CD4 antibody, clone RM4-5 can be used for in vitro blocking of ligand binding, as well as in vitro CD4+ T cell depletions.

Target Species
Mouse
Product Form
Purified IgG conjugated to StarBright Violet 440 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
Immunogen
BALB/c mouse thymocytes
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 440 383 436
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD4 antibody

  1. von Kutzleben, S. et al. (2017) Depletion of CD52-positive cells inhibits the development of central nervous system autoimmune disease, but deletes an immune-tolerance promoting CD8 T-cell population. Implications for secondary autoimmunity of alemtuzumab in multiple sclerosis.
    Immunology. 150 (4): 444-55.
  2. Zamudio, F. et al. (2020) TDP-43 mediated blood-brain barrier permeability and leukocyte infiltration promote neurodegeneration in a low-grade systemic inflammation mouse model.
    J Neuroinflammation. 17 (1): 283.
  3. Aloui, A. et al. (2023) AFM(1) Exposure in Male Balb/c Mice and Intervention Strategies Against Its Immuno-physiological toxicity using Clay Mineral and Lactic Acid Bacteria Alone or in Combination.
    Immunopharmacol Immunotoxicol. : 1-32.

Further Reading

  1. Fehérvari, Z. & Sakaguchi, S. (2004) CD4+ Tregs and immune control.
    J Clin Invest. 114 (9): 1209-17.

Synonyms
L3T4 Antigen
LY-4
UniProt
P06332
Entrez Gene
Cd4
GO Terms
GO:0001816 cytokine production
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0005788 endoplasmic reticulum lumen
GO:0005789 endoplasmic reticulum membrane
GO:0006955 immune response
GO:0007166 cell surface receptor linked signaling pathway
GO:0009897 external side of plasma membrane
GO:0045058 T cell selection
GO:0045121 membrane raft
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050870 positive regulation of T cell activation
GO:0051789 response to protein stimulus

MCA2691SBV440

100004089 64599648

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